Development and characterization of twelve new polymorphic microsatellite loci in the Iberian ribbed newt, Pleurodeles waltl (Caudata: Salamandridae), with data on cross-amplification in P. nebulosus
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3Present address: Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), Rua Padre Armando Quintas, s/n, 4485-661 Vairão, Portugal
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Twelve novel polymorphic microsatellite loci were isolated and characterized for the Iberian ribbed newt, Pleurodeles waltl (Caudata, Salamandridae). The distribution of this newt ranges from central and southern Iberia to northwestern Morocco. Polymorphism of these novel loci was tested in 40 individuals from two Iberian populations and compared with previously published markers. The number of alleles per locus ranged from two to eight. Observed and expected heterozygosity ranged from 0.13 to 0.57 and from 0.21 to 0.64, respectively. Cross-species amplification was tested in Pleurodeles nebulosus, which is listed as Vulnerable by the IUCN. Eight new and seven previously published loci amplified successfully in that species and thus represent a valuable conservation tool. The novel microsatellites will be useful for a better understanding of the population dynamics, demography, genetic structure, and evolutionary history of Pleurodeles waltl and P. nebulosus.
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Development and characterization of twelve new polymorphic microsatellite loci in the Iberian ribbed newt, Pleurodeles waltl (Caudata: Salamandridae), with data on cross-amplification in P. nebulosus
In: Amphibia-ReptiliaSearch for other papers by Jorge Gutiérrez-Rodríguez in
Current site
Google Scholar
PubMed
Search for other papers by Elena G. Gonzalez in
Current site
Google Scholar
PubMed
3Present address: Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), Rua Padre Armando Quintas, s/n, 4485-661 Vairão, Portugal
Search for other papers by Íñigo Martínez-Solano in
Current site
Google Scholar
PubMed
Twelve novel polymorphic microsatellite loci were isolated and characterized for the Iberian ribbed newt, Pleurodeles waltl (Caudata, Salamandridae). The distribution of this newt ranges from central and southern Iberia to northwestern Morocco. Polymorphism of these novel loci was tested in 40 individuals from two Iberian populations and compared with previously published markers. The number of alleles per locus ranged from two to eight. Observed and expected heterozygosity ranged from 0.13 to 0.57 and from 0.21 to 0.64, respectively. Cross-species amplification was tested in Pleurodeles nebulosus, which is listed as Vulnerable by the IUCN. Eight new and seven previously published loci amplified successfully in that species and thus represent a valuable conservation tool. The novel microsatellites will be useful for a better understanding of the population dynamics, demography, genetic structure, and evolutionary history of Pleurodeles waltl and P. nebulosus.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 163 | 0 | 0 |
| Full Text Views | 345 | 66 | 9 |
| PDF Views & Downloads | 107 | 20 | 5 |
