Cryptic diversity of green pitvipers in Yunnan, South-west China (Squamata, Viperidae)

In: Amphibia-Reptilia
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  • 1 College of Life Sciences and Food Engineering, Yibin University, Yibin 644007, China
  • 2 College of Life Sciences, Sichuan University, Chengdu 610064, China
  • 3 College of Tourism and Urban-Rural Planning, Chengdu University of Technology, Chengdu 610059, China
  • 4 State Key Laboratory of Genetic Resources and Evolution, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
  • 5 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China

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The biodiversity of Yunnan, which is located in the Hengduan Mountains region of South-west China, is still underestimated. Here, we explore species diversity of Asian green pitvipers occurring in this region. Based on four mtDNA gene fragments (cyt. b, ND4, 12S RNA, and 16S RNA), we inferred phylogenetic position and relationships of Yunnan green pitvipers using Bayesian Inference (BI) and Maximum Likelihood (ML) methods. Both methods consistently revealed that all examined Yunnan specimens are placed in five clades which should be referred to Trimeresurus albolabris, Viridovipera stejnegeri, V. gumprechti, and the genus Popeia, respectively. It was unexpected that the samples allied to V. gumprechti did not form a monophyletic clade, but rather two well supported, distinct clades. Further morphological comparison suggested that the specimens nested within the clade Popeia should be identified as P. popeiorum, which is a species new to China. As a result, there are currently at least five species occurring in Yunnan, China: Trimeresurus albolabris, Viridovipera stejnegeri, V. yunnanensis, V. gumprechti, and Popeia popeiorum.

  • Arevalo E., Davis S.K., Sites J.W. (1994): Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in central Mexico. Syst. Biol. 43: 387-418.

    • Search Google Scholar
    • Export Citation
  • Burbrink F.T., Lawson R., Slowinski J.B. (2000): Mitochondrial DNA phylogeography of the polytypic North American rat snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution 54: 2107-2118.

    • Search Google Scholar
    • Export Citation
  • David P., Petri M., Vogel G., Doria G. (2009): A new species of pitviper of the genus Trimeresurus (Popeia) from northern Sumatra (Reptilia, Squamata, Viperidae). Annali del Museo Civico di Storia Naturale “G. Doria” 100: 323-346.

    • Search Google Scholar
    • Export Citation
  • David P., Vogel G., Dubois A. (2011): On the need to follow rigorously the Rules of the Code for the subsequent designation of a nucleospecies (type species) for a nominal genus which lacked one: the case of the nominal genus Trimeresurus Lacépède, 1804 (Reptilia: Squamata: Viperidae). Zootaxa 2992: 1-51.

    • Search Google Scholar
    • Export Citation
  • David P., Vogel G., Pauwels O.S.G., Vidal N. (2002): Description of a new species of the genus Trimeresurus from Thailand, related to Trimeresurusstejnegeri Schmidt, 1925 (Serpentes, Crotalidae). Nat. Hist. J. Chulalongkorn Univ. 2: 5-19.

    • Search Google Scholar
    • Export Citation
  • Dowling H.G. (1951): A proposed system for counting ventrals in snakes. British J. Herpetol. 1: 97-99.

  • Gumprecht A., Tillack F., Orlov N.L., Captain A., Ryabov S. (2004): Asian Pitvipers. Geitje Books, Berlin.

  • Guo P., Wang Y.Z. (2011): A new genus and species of cryptic Asian green pitviper (Serpentes: Viperidae: Crotalinae) from southwest China. Zootaxa 2918: 1-14.

    • Search Google Scholar
    • Export Citation
  • Knight A., Mindell D.P. (1993): Substitution bias, weighting of DNA sequence evolution, and the phylogenetic position of Fea’s viper. Syst. Biol. 42: 18-31.

    • Search Google Scholar
    • Export Citation
  • Lanfear R., Calcott B., Ho S.Y.W., Guindon S. (2012): PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Mol. Biol. Evol. 29: 1695-1701.

    • Search Google Scholar
    • Export Citation
  • Malhotra A., Thorpe R.S. (2004): A phylogeny of four mitochondrial gene regions suggests a revised taxonomy for Asian pitvipers (Trimeresurus and Ovophis). Mol. Phylogenet. Evol. 32: 83-100.

    • Search Google Scholar
    • Export Citation
  • Malhotra A., Thorpe R.S. (1997): New perspectives on the evolution of South-East Asian pitvipers, genus Trimeresurus from molecular studies. In: Venomous Snakes: Ecology, Evolution and Snakebite, p.  115-118. Thorpe R.S., Wüster W., Malhotra A., Eds, Clarendon Press, Oxford.

    • Search Google Scholar
    • Export Citation
  • Parkinson C.L., Moody S.M., Ahlquist J.E. (1997): Phylogenetic relationships of the “Agkistrodon complex” based on mitochondrial DNA sequence data. In: Venomous Snakes: Ecology, Evolution and Snakebite, p.  63-78. Thorpe R.S., Wüster W., Malhotra A., Eds, Clarendon Press, Oxford.

    • Search Google Scholar
    • Export Citation
  • Rambaut A., Suchard M.A., Xie D., Drummond A.J. (2014): Tracer v1.6. http://beast.bio.ed.ac.uk/Tracer.

  • Ronquist F., Teslenko M., Mark P.V.D., Ayres D., Darling A., Hohna S., Larget B., Liu L., Suchard M.A., Huelsenbeck J.P. (2012): MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61 (3): 539-542.

    • Search Google Scholar
    • Export Citation
  • Sambrook J., Russell D.W. (2002): Molecular Cloning: a Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    • Search Google Scholar
    • Export Citation
  • Smith M.A. (1937): The names of two Indian vipers. J. Bombay Nat. Hist. Soc. 39: 730-731.

  • Stamatakis A. (2006): RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688-2690.

    • Search Google Scholar
    • Export Citation
  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (2013): MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729.

    • Search Google Scholar
    • Export Citation
  • Uetz P., Hallermann J. (2015): Reptile database. http://reptile-database.reptarium.cz. Accessed on March 01, 2015.

  • Vogel G., David P. (2010): A new species of the genus Lycodon (Boie, 1826) from Yunnan Province, China (Serpentes: Colubridae). Bonn Zool. Bull. 57 (2): 289-296.

    • Search Google Scholar
    • Export Citation
  • Vogel G., David P., Pauwels O.S.G. (2004): A review of morphological variation in Trimeresurus popeiorum (Serpentes: Viperidae: Crotalinae), with the description of two new species. Zootaxa 727: 1-63.

    • Search Google Scholar
    • Export Citation
  • Vogel G., Luo J. (2011): A new species of the genus Lycodon (Boie, 1826) from the southwestern mountains of China (Squamata: Colubridae). Zootaxa 2807: 29-40.

    • Search Google Scholar
    • Export Citation
  • Yang D.T., Rao D.Q. (2008): Amphibia and Reptilia of Yunnan. Yunnan Science and Technology Press, Kunming.

  • Zhang D.X., Hewitt G.M. (1996): Nuclear integrations: challenges for mitochondrial DNA markers. TREE 11: 247-251.

  • Zhao E.M. (2006): Snakes of China. Anhui Science and Technology Publishing House, Hefei.

  • Zhao E.M., Huang M.H., Zong Y., Eds (1998): Fauna Sinica, Reptilia, Squamata. Serpentes, Vol. 3. Science Press, Beijing.

  • Zhao E.M., Yang D.T. (1997): Amphibians and Reptilies of the Hengduan Mountains Region. Science Press, Beijing.

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