Avoiding food-borne diseases by competitive exclusion agents is a proactive strategy. In the current paper, we report the use of Bacillus smithii TBMI12 spores as potential competitive exclusion agents. One group of mice was predosed for three successive days with 108 colony forming units of B. smithii TBMI12 spores followed by inoculation with 106 colony forming units of wild-type Salmonella enterica serotype Enteritidis cells. Microbial plate counts of the animals' livers and spleens showed that only 40% of the mice were infected with S. enterica serotype Enteritidis, while the control group was 100% infected. These results suggest that B. smithii TBMI12 spores may protect against infection by S. enterica serotype Enteritidis.
Bohnhoff, M., Miller, C.P. and Martin, W.R., 1964. Resistance of the mouse's intestinal tract to experimental Salmonella infection. II. Factors responsible for its loss following streptomycin treatment. The Journal of Experimental Medicine 120: 817-828.
'Resistance of the mouse's intestinal tract to experimental Salmonella infection ' () 120 II. Factors responsible for its loss following streptomycin treatment. The Journal of Experimental Medicine : 817 -828.
Casula, G. and Cutting, M.S., 2002. Bacillus probiotics: spore germination in the gastrointestinal tract. Applied and Environmental Microbiology 68: 2344-2352.
Bacillus , ().
Council of Europe, 1986. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. European Treaty Series 123. Available at: http://conventions.coe.int/treaty/EN/Treaties/Html/123.htm
Darwin, K.H. and Miller, V.L., 1999. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clinical Microbiology Reviews 12: 405-428.
'Molecular basis of the interaction of Salmonella with the intestinal mucosa ' () 12 Clinical Microbiology Reviews : 405 -428.
Duc, L.H., Hong, H.A., Barbosa, T.M., Henriques, A.O. and Cutting, S.M., 2004. Characterization of Bacillus probiotics available for human use. Applied and Environmental Microbiology 70: 2161-2171.
'Characterization of Bacillus probiotics available for human use ' () 70 Applied and Environmental Microbiology : 2161 -2171.
European Commission (EC), 2003. Regulation No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. Official Journal of the European Union L268: 29-43.
European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC), 2006. The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2005. The EFSA Journal 94: 80-84.
Hong, H.A., Duc, L.H. and Cutting, S.M., 2005. The use of bacterial spore formers as probiotics. FEMS Microbiology Letters 29: 813-835.
'The use of bacterial spore formers as probiotics ' () 29 FEMS Microbiology Letters : 813 -835.
Humphrey, T., 2006. Public health aspects of Salmonella enterica in food production. In: Mastroeni, P. and Maskell, D. (eds.) Salmonella infections, clinical, immunological and molecular aspects. Cambridge University Press, Cambridge, UK, pp. 89-116.
Kyriakis, S.C., Tsiloyiannis, V.S., Vlemmas, J., Sarris, K., Tsinas, A.C., Alexopoulos, C. and Jansegers, L., 1999. The effect of probiotic LSP122 on the control of post-weaning diarrhoea syndrome in piglets. Research in Veterinary Science 67: 223-228.
La Ragione, R.M., Casula, G., Cutting, S.M. and Woodward, M.J., 2001. Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry. Veterinary Microbiology 79: 133-142.
'Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry ' () 79 Veterinary Microbiology : 133 -142.
La Ragione, R.M. and Woodward, M.J., 2003. Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens. Veterinary Microbiology 94: 245-256.
'Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens ' () 94 Veterinary Microbiology : 245 -256.
Mead, P.S., Slutsker, L., Diez, V., McCaig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M. and Tauxe, R.V., 1999. Food-related illness and death in the United States. Emerging Infectious Diseases 5: 607-625.
'Food-related illness and death in the United States ' () 5 Emerging Infectious Diseases : 607 -625.
Michelson, T., Kask, K., Jõgi, E., Talpsep, E., Suitso, I. and Nurk, A., 2006. L(+)-Lactic acid producer Bacillus coagulans SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii ssp. lactis DSM20073. Enzyme and Microbial Technology 39: 861-867.
Nicholson, W.L. and Setlow P., 1990. Sporulation, germination, and outgrowth. In: Harwood, C.R. and Cutting, S.M. (eds.) Molecular biological methods for Bacillus. John Wiley & Sons, Sussex, UK, pp. 391-450.
Park, J.-H., Seok, S.-H., Cho, S.-A., Baek, M.-W., Lee, H.-Y., Kim, D.-J., Chung, M.-J., Kim, S.-D., Hong, U.-P. and Park, J.-H., 2005. Antimicrobial effect of lactic acid producing bacteria culture condensate mixture (LCCM) against Salmonella enteritidis. International Journal of Food Microbiology 101: 111-117.
Soumet, C., Ermel, G.., Rose, N., Drouin, P., Salvat, G. and Colin, P., 1999. Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses. Letters in Applied Microbiology 29: 1-6.
'Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses ' () 29 Letters in Applied Microbiology : 1 -6.
Spinosa, M.R., Braccini, T., Ricca, E., De Felice, M., Morelli, L., Pozzi, G. and Oggioni, M.R., 2000. On the fate of ingested Bacillus spores. Research in Microbiology 151: 361-368.
Vedler, E., Kõiv, V. and Heinaru, A., 2000. Analysis of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pEST4011 of Achromobacter xylosoxidans subsp. denitrificans strain EST4002. Gene 255: 281-288.
Wolken, W.A.M., Tramper, J. and Van der Werf, M.J., 2003. What can spores do for us? Trends in Biotechnology 21: 338-345.
'What can spores do for us ' () 21 Trends in Biotechnology : 338 -345.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 0 | 0 | 0 |
Full Text Views | 31 | 31 | 7 |
PDF Views & Downloads | 15 | 14 | 0 |
Avoiding food-borne diseases by competitive exclusion agents is a proactive strategy. In the current paper, we report the use of Bacillus smithii TBMI12 spores as potential competitive exclusion agents. One group of mice was predosed for three successive days with 108 colony forming units of B. smithii TBMI12 spores followed by inoculation with 106 colony forming units of wild-type Salmonella enterica serotype Enteritidis cells. Microbial plate counts of the animals' livers and spleens showed that only 40% of the mice were infected with S. enterica serotype Enteritidis, while the control group was 100% infected. These results suggest that B. smithii TBMI12 spores may protect against infection by S. enterica serotype Enteritidis.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 0 | 0 | 0 |
Full Text Views | 31 | 31 | 7 |
PDF Views & Downloads | 15 | 14 | 0 |