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Protective effect by Bacillus smithii TBMI12 spores of Salmonella serotype Enteritidis in mice

In: Beneficial Microbes
Authors:
I. Suitso 1Institute of Technology, University of Tartu, Nooruse 1-301, 50411 Tartu, Estonia
indrek.suitso@ut.ee

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E. Jõgi 1Institute of Technology, University of Tartu, Nooruse 1-301, 50411 Tartu, Estonia

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E. Talpsep 1Institute of Technology, University of Tartu, Nooruse 1-301, 50411 Tartu, Estonia

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P. Naaber 2Department of Clinical Microbiology, United Laboratories of Tartu University Clinics, Estonia
3Department of Medical Microbiology, Stavanger University Hospital, Norway

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K. Lõivukene 2Department of Clinical Microbiology, United Laboratories of Tartu University Clinics, Estonia

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M.-L. Ots 1Institute of Technology, University of Tartu, Nooruse 1-301, 50411 Tartu, Estonia

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T. Michelson 4Institute of Molecular and Cell Biology, University of Tartu, Estonia

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A. Nurk 1Institute of Technology, University of Tartu, Nooruse 1-301, 50411 Tartu, Estonia

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Open Access

Abstract

Avoiding food-borne diseases by competitive exclusion agents is a proactive strategy. In the current paper, we report the use of Bacillus smithii TBMI12 spores as potential competitive exclusion agents. One group of mice was predosed for three successive days with 108 colony forming units of B. smithii TBMI12 spores followed by inoculation with 106 colony forming units of wild-type Salmonella enterica serotype Enteritidis cells. Microbial plate counts of the animals' livers and spleens showed that only 40% of the mice were infected with S. enterica serotype Enteritidis, while the control group was 100% infected. These results suggest that B. smithii TBMI12 spores may protect against infection by S. enterica serotype Enteritidis.

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