The effects of Lactobacillus acidophilus NCFM™, lactitol, and the combination of lactitol and L. acidophilus NCFM™ were studied with a semi-continuous colon fermentation simulation; consisting of compartments mimicking, ascending, transverse, descending and sigmoid colon and their conditions with faecal inoculation. L. acidophilus NCFM™ was detected throughout the colon simulator. Lactitol was utilised early on by the microbes in the proximal part of the simulator. Lactitol increased the total numbers of microbes and bifidobacteria, and decreased clostridia cluster IV, while L. acidophilus NCFM™ alone decreased the numbers of clostridia cluster XIV. Combination treatment increased the numbers of bifidobacteria. Furthermore, concentrations of acetic acid, butyric acid and the sum of total short-chain fatty acids were increased by both lactitol-including treatments. The treatment with L. acidophilus NCFM™ alone increased the concentration of propionic acid and butyric acid. L. acidophilus NCFM™ tended to increase the total concentrations of biogenic amines, while lactitol suppressed production of biogenic amines also in the presence of L. acidophilus NCFM™. True synergistic effects are suggested in stimulation of the production of butyrate, an important microbial metabolite for colon health. In conclusion, lactitol as well as the combination of lactitol and L. acidophilus NCFM™ were found to exhibit complementary beneficial effects on the colon microbial composition and activity.
Apajalahti, J., Kettunen, H., Kettunen, A., Holben, W. N.P., Rautonen, N. and Mutanen, M., 2002. Culture-independent microbial community analysis reveals that inulin in the diet primarily affects previously unknown bacteria in the mouse caecum. Applied and Environmental Microbiology 68: 4986-4995.
'Culture-independent microbial community analysis reveals that inulin in the diet primarily affects previously unknown bacteria in the mouse caecum ' () 68 Applied and Environmental Microbiology : 4986 -4995.
Crittenden, R., Morris, L., Harvey, M., Tran, L., Mitchell, H. and Playne, M., 2001. Selection of a Bifidobacterium strain to complement resistant starch in a synbiotic yoghurt. Journal of Applied Microbiology 90: 268-278.
'Selection of a Bifidobacterium strain to complement resistant starch in a synbiotic yoghurt ' () 90 Journal of Applied Microbiology : 268 -278.
Drakoularakou, A., Hasselwarder, O., Edinburgh, M. and Ouwehand, A.C., 2007. Lactitol, an emerging prebiotic: functional properties with a focus on digestive health. Food Science Technological Bulletin 3: 71-80.
'Lactitol, an emerging prebiotic: functional properties with a focus on digestive health ' () 3 Food Science Technological Bulletin : 71 -80.
Dunn, S., Simenhoff, M., Ahmed, K., Gaughan, W., Eltayeb, B., Fitzpatrick, M., Emery, S., Ayres, J. and Holt, K., 1998. Effect of oral administration of freeze-dried Lactobacillus acidophilus on small bowel bacterial overgrowth in patients with end stage kidney disease: reducing uremic toxins and improving nutrition. International Dairy Journal 8: 545-553.
'Effect of oral administration of freeze-dried Lactobacillus acidophilus on small bowel bacterial overgrowth in patients with end stage kidney disease: reducing uremic toxins and improving nutrition ' () 8 International Dairy Journal : 545 -553.
Fava, F., Mäkivuokko, H., Siljander-Rasi, H., Putaala, H., Tiihonen, K., Stowell, J., Tuohy, K., Gibson, G. and Rautonen, N., 2007. Effect of polydextrose on intestinal microbes and immune functions in pigs. British Journal of Nutrition 98: 123-133.
'Effect of polydextrose on intestinal microbes and immune functions in pigs ' () 98 British Journal of Nutrition : 123 -133.
Finney, M., Smullen, J., Foster, H., Brokx, S. and Storey, D. 2007. Effects of low doses of lactitol on faecal microflora, pH, short chain fatty acids and gastrointestinal symptomology. European Journal of Nutrition 46: 307-314.
'Effects of low doses of lactitol on faecal microflora, pH, short chain fatty acids and gastrointestinal symptomology ' () 46 European Journal of Nutrition : 307 -314.
Foligne, B., Nutten, S., Grangette, C., Dennin, V., Goudercourt, D., Poiret, S., Dewulf, J., Brassart, D., Mercenier, A. and Pot, B., 2007. Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria. World Journal of Gastroenterology 13: 236-243.
'Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria ' () 13 World Journal of Gastroenterology : 236 -243.
Gmeiner, M., Kneifel, W., Kulbe, K., Wouters, R., De Boever, P., Nollet, L. and Verstraete, W., 2000. Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor). Applied Microbiology and Biotechnology 53: 219-223.
'Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor) ' () 53 Applied Microbiology and Biotechnology : 219 -223.
Hotten, P., Marotta, F., Naito, Y., Minelli, E., Helmy, A., Lighthouse, J., Fuji, H. and Fesce, E., 2003. Effects of probiotics, lactitol and rifaximin on intestinal flora and fecal excretion of organic acids in cirrhotic patients. Chinese Journal of Digestive Diseases 4: 13-18.
'Effects of probiotics, lactitol and rifaximin on intestinal flora and fecal excretion of organic acids in cirrhotic patients ' () 4 Chinese Journal of Digestive Diseases : 13 -18.
Islam, M., Sakaguchi, E., Kashima, N. and Hoshi, S., 2004. Effect of sugar alcohols on gut function and body composition in normal and cecectomized rats. Experimental Animals / Japanese Association for Laboratory Animal Science 53: 361-371.
'Effect of sugar alcohols on gut function and body composition in normal and cecectomized rats ' () 53 Experimental Animals / Japanese Association for Laboratory Animal Science : 361 -371.
Macfarlane, G., Macfarlane, S. and Gibson, G., 1998. Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon. Microbial Ecology 35: 180-187.
'Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon ' () 35 Microbial Ecology : 180 -187.
Mäkeläinen, H., Forssten, S., Olli, K., Granlund, L., Rautonen, N. and Ouwehand, A., 2009. Probiotic lactobacilli in a semi-soft cheese survive in the simulated human gastrointestinal tract. International Dairy Journal 19: 675-683.
'Probiotic lactobacilli in a semi-soft cheese survive in the simulated human gastrointestinal tract ' () 19 International Dairy Journal : 675 -683.
Mäkeläinen, H., Mäkivuokko, H., Salminen, S., Rautonen, N. and Ouwehand, A., 2007. The effects of polydextrose and xylitol on microbial community and activity in a 4-stage colon simulator. Journal of Food Science 72: M153-M159.
'The effects of polydextrose and xylitol on microbial community and activity in a 4-stage colon simulator ' () 72 Journal of Food Science : M153 -M159.
Mäkivuokko, H., Kettunen, H., Saarinen, M., Kamiwaki, T., Yokoyama, Y., Stowell, J. and Rautonen, N., 2007. The effect of cocoa and polydextrose on bacterial fermentation and growth in gastrointestinal tract simulations. Bioscience, Biotechnology and Biochemistry 71: 1834-1843.
'The effect of cocoa and polydextrose on bacterial fermentation and growth in gastrointestinal tract simulations ' () 71 Bioscience, Biotechnology and Biochemistry : 1834 -1843.
Mäkivuokko, H., Saarinen, M., Ouwehand, A. and Rautonen, N., 2006. Effects of lactose on colon microbial community structure and function in a four-stage semi-continuous culture system. Bioscience, Biotechnology and Biochemistry 70: 2056-2063.
'Effects of lactose on colon microbial community structure and function in a four-stage semi-continuous culture system ' () 70 Bioscience, Biotechnology and Biochemistry : 2056 -2063.
Mäkivuokko, H., Nurmi, J., Nurminen, P., Stowell, J. and Rautonen, N., 2005. In vitro effects on polydextrose by colonic bacteria and Caco-2 cell cyclooxygenase gene expression. Nutrition and Cancer 52: 94-104.
'In vitro effects on polydextrose by colonic bacteria and Caco-2 cell cyclooxygenase gene expression ' () 52 Nutrition and Cancer : 94 -104.
Matsuki, T., Watanabe, K., Fujimoto, J., Kado, Y., Takada, T., Matsumoto, K. and Tanaka, R., 2004. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. Applied and Environmental Microbiology 70: 167-173.
'Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria ' () 70 Applied and Environmental Microbiology : 167 -173.
Oku, T., Nakamura, S. and Ichinose, M., 2005. Maximum permissive dosage of lactose and lactitol for transitory diarrhea, and utilizable capacity for lactose in Japanese female adults. Journal of Nutritional Science and Vitaminology 51: 51-57.
'Maximum permissive dosage of lactose and lactitol for transitory diarrhea, and utilizable capacity for lactose in Japanese female adults ' () 51 Journal of Nutritional Science and Vitaminology : 51 -57.
Ouwehand, A., Tiihonen, K., Mäkivuokko, H. and Rautonen, N., 2007. Synbiotics: combining the benefits of pre-and probiotics. In: Saarela, M. (ed.) Functional dairy products, Vol. 2. Woodhead Publishing Ltd., Cambridge, UK, pp. 195-213.
Ouwehand, A.C., Tiihonen, K., Saarinen, M., Putaala, H. and Rautonen, N., 2009. Influence of a combination of Lactobacillus acidophilus NCFM and lactitol on healthy elderly: intestinal and immune parameters. The British journal of Nutrition 101: 367-375.
'Influence of a combination of Lactobacillus acidophilus NCFM and lactitol on healthy elderly: intestinal and immune parameters ' () 101 The British journal of Nutrition : 367 -375.
Priebe, M.G., Vonk, R.J., Sun, X., He, T., Harmsen, H.J. and Welling, G.W., 2002. The physiology of colonic metabolism. Possibilities for interventions with pre-and probiotics. European Journal of Nutrition 41 (Suppl. 1): 1101-1108.
Saarinen, M.T., 2002. Determination of biogenic amines as dansyl derivatives in intestinal digesta and feces by reversed phase HPLC. Chromatographia 55: 297-300.
'Determination of biogenic amines as dansyl derivatives in intestinal digesta and feces by reversed phase HPLC ' () 55 Chromatographia : 297 -300.
Sanders, M. and Klaenhammer, T., 2001. Invited review: the scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic. Journal of Dairy Science 84: 319-331.
'Invited review: the scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic ' () 84 Journal of Dairy Science : 319 -331.
Scheppach, W. and Weiler, F., 2004. The butyrate story: old wine in new bottles? Current Opinion in Clinical Nutrition and Metabolic Care 7: 563-567.
'The butyrate story: old wine in new bottles ' () 7 Current Opinion in Clinical Nutrition and Metabolic Care : 563 -567.
Smith, E. and Macfarlane, G., 1997a. Formation of phenolic and indolic compounds by anaerobic bacteria in the human large intestine. Microbial Ecology 33: 180-188.
'Formation of phenolic and indolic compounds by anaerobic bacteria in the human large intestine ' () 33 Microbial Ecology : 180 -188.
Smith, E. and Macfarlane, G., 1997b. Dissimilatory amino acid metabolism in human colonic bacteria. Anaerobe 3: 327-337.
'Dissimilatory amino acid metabolism in human colonic bacteria ' () 3 Anaerobe : 327 -337.
Song, Y., Liu, C. and Finegold, S., 2004. Real-time PCR quantitation of clostridia in feces of autistic children. Applied and Environmental Microbiology 70: 6459-6465.
'Real-time PCR quantitation of clostridia in feces of autistic children ' () 70 Applied and Environmental Microbiology : 6459 -6465.
All Time | Past 365 days | Past 30 Days | |
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The effects of Lactobacillus acidophilus NCFM™, lactitol, and the combination of lactitol and L. acidophilus NCFM™ were studied with a semi-continuous colon fermentation simulation; consisting of compartments mimicking, ascending, transverse, descending and sigmoid colon and their conditions with faecal inoculation. L. acidophilus NCFM™ was detected throughout the colon simulator. Lactitol was utilised early on by the microbes in the proximal part of the simulator. Lactitol increased the total numbers of microbes and bifidobacteria, and decreased clostridia cluster IV, while L. acidophilus NCFM™ alone decreased the numbers of clostridia cluster XIV. Combination treatment increased the numbers of bifidobacteria. Furthermore, concentrations of acetic acid, butyric acid and the sum of total short-chain fatty acids were increased by both lactitol-including treatments. The treatment with L. acidophilus NCFM™ alone increased the concentration of propionic acid and butyric acid. L. acidophilus NCFM™ tended to increase the total concentrations of biogenic amines, while lactitol suppressed production of biogenic amines also in the presence of L. acidophilus NCFM™. True synergistic effects are suggested in stimulation of the production of butyrate, an important microbial metabolite for colon health. In conclusion, lactitol as well as the combination of lactitol and L. acidophilus NCFM™ were found to exhibit complementary beneficial effects on the colon microbial composition and activity.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 0 | 0 | 0 |
Full Text Views | 139 | 122 | 16 |
PDF Views & Downloads | 111 | 79 | 2 |