Intramammary inoculation with lactic acid bacteria at dry-off triggers an immunomodulatory response in dairy cows
In: Beneficial MicrobesMember of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Godoy Cruz 2290, C1425FQB-Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
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CIT-VM (Centro de Investigaciones y Transferencia de Villa María, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María), Av. Arturo Jauretche 1555, 5900-Villa María, Córdoba, Argentina.
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CIT-VM (Centro de Investigaciones y Transferencia de Villa María, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María), Av. Arturo Jauretche 1555, 5900-Villa María, Córdoba, Argentina.
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CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina), Departamento de Microbiología Preventiva, Chacabuco 145, 4000- San Miguel de Tucumán, Argentina.
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Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Godoy Cruz 2290, C1425FQB-Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
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The use of antibiotics to prevent bovine mastitis is responsible for the emergence and selection of resistant strains. Lactic acid bacteria (LAB) could be introduced into animal feed as an alternative prevention method that would bypass the risk of resistance development. In previous research, we demonstrated that two probiotic LAB strains isolated from bovine milk were capable of stimulating the production of antibodies and the host’s immune cellular response in the udder. The present study aimed to elucidate whether the antibodies of animals inoculated with these strains were able to increase phagocytosis by neutrophils and inhibit the growth of different mastitis-causing pathogens. Moreover, the effect of LAB on the expression of pro-inflammatory cytokines was assessed. Ten animals were inoculated intramammarily with 106 cells of the two strains at dry-off. The blood serum was tested for its ability to opsonize bovine mastitis pathogens, the in vitro bactericidal activity of bovine blood and milk against these pathogens was determined, and cytokine mRNA expression was quantified in milk somatic cells. The inoculated animals did not show abnormal signs of sensitivity to the LAB. Their blood serum significantly enhanced the phagocytosis of Staphylococcus spp. and the LAB. Escherichia coli and Streptococcus uberis were inhibited by the milk serum but not the blood serum, whereas Staphylococcus aureus and Staphylococcus haemolyticus were inhibited by both. In regard to cytokine expression, interleukin (IL)-1β increased markedly for up to 4 h post-inoculation, and an increase in IL-8 was observed 4, 12 and 24 h after inoculation. Tumour necrosis factor-α mRNA increased 1 and 2 h after inoculation and a significant difference was registered at 6 h for interferon-γ. This rapid immunomodulatory response shows that inoculating animals with LAB at dry-off, when they are especially susceptible, could be a useful strategy for the prevention of bovine mastitis.
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Intramammary inoculation with lactic acid bacteria at dry-off triggers an immunomodulatory response in dairy cows
In: Beneficial MicrobesMember of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Godoy Cruz 2290, C1425FQB-Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
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CIT-VM (Centro de Investigaciones y Transferencia de Villa María, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María), Av. Arturo Jauretche 1555, 5900-Villa María, Córdoba, Argentina.
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CIT-VM (Centro de Investigaciones y Transferencia de Villa María, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María), Av. Arturo Jauretche 1555, 5900-Villa María, Córdoba, Argentina.
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CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina), Departamento de Microbiología Preventiva, Chacabuco 145, 4000- San Miguel de Tucumán, Argentina.
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Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Godoy Cruz 2290, C1425FQB-Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
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The use of antibiotics to prevent bovine mastitis is responsible for the emergence and selection of resistant strains. Lactic acid bacteria (LAB) could be introduced into animal feed as an alternative prevention method that would bypass the risk of resistance development. In previous research, we demonstrated that two probiotic LAB strains isolated from bovine milk were capable of stimulating the production of antibodies and the host’s immune cellular response in the udder. The present study aimed to elucidate whether the antibodies of animals inoculated with these strains were able to increase phagocytosis by neutrophils and inhibit the growth of different mastitis-causing pathogens. Moreover, the effect of LAB on the expression of pro-inflammatory cytokines was assessed. Ten animals were inoculated intramammarily with 106 cells of the two strains at dry-off. The blood serum was tested for its ability to opsonize bovine mastitis pathogens, the in vitro bactericidal activity of bovine blood and milk against these pathogens was determined, and cytokine mRNA expression was quantified in milk somatic cells. The inoculated animals did not show abnormal signs of sensitivity to the LAB. Their blood serum significantly enhanced the phagocytosis of Staphylococcus spp. and the LAB. Escherichia coli and Streptococcus uberis were inhibited by the milk serum but not the blood serum, whereas Staphylococcus aureus and Staphylococcus haemolyticus were inhibited by both. In regard to cytokine expression, interleukin (IL)-1β increased markedly for up to 4 h post-inoculation, and an increase in IL-8 was observed 4, 12 and 24 h after inoculation. Tumour necrosis factor-α mRNA increased 1 and 2 h after inoculation and a significant difference was registered at 6 h for interferon-γ. This rapid immunomodulatory response shows that inoculating animals with LAB at dry-off, when they are especially susceptible, could be a useful strategy for the prevention of bovine mastitis.
| All Time | Past 365 days | Past 30 Days | |
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