The microbiota of the gastrointestinal tract (GIT) constitutes the major part of the total human microbiome and is considered to be an important regulator of human health and host metabolism. Numerous investigations in recent years have focused on the connection between the human microbiota and metabolic diseases such as obesity, type II diabetes and atherosclerosis. Yet, little is known about the impact of probiotic consumption on the GIT microbial population and the potential effect on chronic diseases. In this study, the modulation of the microbial community in the murine small intestine resulting from probiotic feeding was investigated and was found to be associated with an anti-obesity effect. Changes in the microbiota of the mouse faeces and small intestine were monitored using quantitative real-time PCR and by following the mRNA expression levels of various obesity-related biomarkers following probiotic feeding in a mouse model. Lactobacillus rhamnosus GG and Lactobacillus sakei NR28 (a putative probiotic strain isolated from kimchi) were administered at a daily level of approximately 1×108 viable bacteria per mouse (C57BL/6J mice) for up to three weeks. Feeding these strains resulted in a significant reduction of epididymal fat mass, as well as obesity-related biomarkers like acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase-1 in the liver. The total number and ratio of the microbial groups, i.e. Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp. were modulated in the small intestine, and the Firmicutes:Bacteroidetes ratio was decreased. In contrast, no noticeable effect of probiotic feeding could be detected on the faecal microbiota, neither quantitatively, nor with regard to the bacterial groups (Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp.) studied.
Armougom, F., Henry, M., Vialettes, B., Raccah, D. and Raoult, D., 2009. Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and methanogens in anorexic patients. PLoS One 4: e7125.
'Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and methanogens in anorexic patients ' () 4 PLoS One : e7125.
Bäckhed, F., Ding, H., Wang, T., Hooper, L.V., Koh, G.Y., Nagy, A., Semenkovich, C.F. and Gordon, J.I., 2004. The gut microbiota as an environmental factor that regulates fat storage. Proceedings of the National Academy of Sciences of the USA 101: 15718-15723.
'The gut microbiota as an environmental factor that regulates fat storage ' () 101 Proceedings of the National Academy of Sciences of the USA : 15718 -15723.
Delroisse, J.-M., Boulvin, A.-L., Parmentier, I., Dauphin, R.D., Vandenbol, M. and Portetelle, D., 2008. Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR. Microbiological Research 163: 663-670.
'Quantification of Bifidobacterium spp ' () 163 and Lactobacillus spp. in rat fecal samples by real-time PCR. Microbiological Research : 663 -670.
DiBaise, J.K., Zhang, H., Crowell, M.D., Krajmalnik-Brown, R., Decker, G.A. and Rittmann, B.E., 2008. Gut microbiota and its possible relationship with obesity. Mayo Clinical Proceedings 83: 460-469.
'Gut microbiota and its possible relationship with obesity ' () 83 Mayo Clinical Proceedings : 460 -469.
Dick, L.K. and Field, K.G., 2004. Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rRNA genes. Applied and Environmental Microbiology 70: 5695-5697.
'Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rRNA genes ' () 70 Applied and Environmental Microbiology : 5695 -5697.
Duncan, S.H., Lobley, G.E., Holtrop, G., Ince, J., Johnstone, A.M., Louis, P. and Flint, H.J., 2008. Human colonic microbiota associated with diet, obesity and weight loss. International Journal of Obesity 32: 1720-1724.
'Human colonic microbiota associated with diet, obesity and weight loss ' () 32 International Journal of Obesity : 1720 -1724.
Eckburg, P.B., Bik, E.M., Bernstein, C.N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S.R., Nelson, K.E. and Relman, D.A., 2005. Diversity of the human intestinal microbial flora. Science 308: 1635-1638.
'Diversity of the human intestinal microbial flora ' () 308 Science : 1635 -1638.
Erhlich, S.D., 2009. Little evidence for a link to obesity. Nature Reviews Microbiology 7: 901.
'Little evidence for a link to obesity ' () 7 Nature Reviews Microbiology : 901.
Gill, S.R., Pop, M., Deboy, R.T., Eckurg, P.B., Turnbaugh, P.J., Samuel, B.S., Gordon, J.I., Relman, D.A., Fraser-Liggett, F. and Nelson, K.E.,2006. Metagenomic analysis of the human distal gut microbiome. Science 312: 1355-1359.
'Metagenomic analysis of the human distal gut microbiome ' () 312 Science : 1355 -1359.
Gionchetti, P., Rizzello, F., Venturi, A., Brigidi, P., Matteuzzi, D., Bazzocchi, G., Poggioli, G., Miglioli, M. and Campieri, M., 2000. Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo controlled trial. Gastroenterology 119: 305-309.
'Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo controlled trial ' () 119 Gastroenterology : 305 -309.
Goidin, D., Mamessier, A., Staque, M.-J., Schmitt, D. and Berthier-Vergnes, O., 2001. Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and β-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Analytical Biochemistry 295: 17-21.
'Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and β-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations ' () 295 Analytical Biochemistry : 17 -21.
Goodman, A.L., Kallstrom, G., Faith, J.J., Reyes, A., Moore, A., Dantas, G. and Gordon, J.I., 2011. Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice. Proceedings of the National Academy of Sciences of the USA 108: 6252-6257.
'Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice ' () 108 Proceedings of the National Academy of Sciences of the USA : 6252 -6257.
Haakensen, M., Dobson, C.M., Deneer, H. and Ziola, B., 2008. Real-time PCR detection of bacteria belonging to the Firmicutes Phylum. International Journal of Food Microbiology 125: 236-241.
'Real-time PCR detection of bacteria belonging to the Firmicutes Phylum ' () 125 International Journal of Food Microbiology : 236 -241.
Haarman, M. and Knol, J., 2006. Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula. Applied and Environmental Microbiology 72: 2359-2365.
'Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula ' () 72 Applied and Environmental Microbiology : 2359 -2365.
Hirsch, A. and Grinsted, E., 1954. Methods for the growth and enumeration of anaerobic sporeformers from cheese, with observations on the effect of nisin. Journal of Dairy Research 21: 101-110.
'Methods for the growth and enumeration of anaerobic sporeformers from cheese, with observations on the effect of nisin ' () 21 Journal of Dairy Research : 101 -110.
Holdeman, L.V. and Moore, W.E.C. (eds.), 1975. Anaerobe laboratory manual. 3rd ed. Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Anaerobe laboratory manual
Horton, J.D., Goldstein, J.L. and Brown, M.S., 2002. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. Journal of Clinical Investigation 109: 1125-1131.
'SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver ' () 109 Journal of Clinical Investigation : 1125 -1131.
Isolauri, E., Arvilommi, H. and Salminen, S., 1999. Gastrointestinal infections. In: Gibson, G.R. and Roberfroid, M.B. (eds.) Colonic microbiota, nutrition and health. Kluwer Academic Publishers, Dordrecht, the Netherlands, pp. 267-279.
'Gastrointestinal infections ', in Colonic microbiota, nutrition and health , () 267 -279.
Khan, M., Raoult, D., Richet, H., Lepidi, H. and La Scola., B., 2007. Growth-promoting effects of single-dose intragastrically administered probiotics in chickens. British Poultry Science 48: 732-735.
'Growth-promoting effects of single-dose intragastrically administered probiotics in chickens ' () 48 British Poultry Science : 732 -735.
Larsen, N., Bogensen, F.K., Van den Berg, F.W.J., Nielsen, D.S., Andreasen, A.S., Pedersen, B.K., Al-Soud, W.A., Sørensen, S.J., Hansen, L.H. and Jakobsen, M., 2010. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS One 5: e9085.
'Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults ' () 5 PloS One : e9085.
Lee, H.J., Yoon, H.S., Ji, Y., Kim, H., Park, H., Lee, J., Shin, H. and Holzapfel, W.H., 2011. Functional properties of lactic acid bacteria isolated from kimchi. International Journal of Food Microbiology 145: 155-161.
'Functional properties of lactic acid bacteria isolated from kimchi ' () 145 International Journal of Food Microbiology : 155 -161.
Ley, R.E., Bäckhed, F., Turnbaugh, P.J., Lozupone, C.A., Knight, R.D. and Gordon, J.I., 2005. Obesity alters gut microbial ecology. Proceedings of the National Academy of Sciences of the USA 102: 11070-11075.
'Obesity alters gut microbial ecology ' () 102 Proceedings of the National Academy of Sciences of the USA : 11070 -11075.
Ley, R.E., Turnbaugh, P.J., Klein, S. and Gordon, J.I., 2006. Microbial ecology: human gut microbes associated with obesity. Nature 444: 1022-1023.
'Microbial ecology: human gut microbes associated with obesity ' () 444 Nature : 1022 -1023.
Livak, K.J. and Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta Delta C(t)] method. Methods 25: 402-408.
'Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta Delta C(t)] method ' () 25 Methods : 402 -408.
Lyra, A., Lahtinen, S., Tiihonen, K. and Ouwehand, A.C., 2010. Intestinal microbiota and overweight. Beneficial Microbes 1: 407-421.
'Intestinal microbiota and overweight ' () 1 Beneficial Microbes : 407 -421.
Mariat, D., Firmesse, O., Levenez, F., Guimarăes, V.D., Sokol, H., Doré, J., Corthier, G. and Furet, J.-P., 2009. The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age. BMC Microbiology 9: 123.
'The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age ' () 9 BMC Microbiology : 123.
Martin, P.G.P., Guillou, H., Lasserre, F., Déjean, S.L.A., Pascussi, J.-M., SanCristobal, M., Legrand, P., Sesse, P. and Pineau, T., 2007. Novel aspects of PPARα-mediated regulation of lipid and xenobiotic metabolism revealed through a nutrigenomic study. Hepatology 45: 767-777.
'Novel aspects of PPARα-mediated regulation of lipid and xenobiotic metabolism revealed through a nutrigenomic study ' () 45 Hepatology : 767 -777.
Matijašic, B.B., Rajšp, M.K., Perko, B. and Rogelj, I., 2006. Inhibition of Clostridium tyrobutyricum in cheese by Lactobacillus gasseri. International Dairy Journal 17: 157-166.
'Inhibition of Clostridium tyrobutyricum in cheese by Lactobacillus gasseri ' () 17 International Dairy Journal : 157 -166.
Naaber, P., Smidt, I., Štšepetova, J., Brilene, T., Annuk, H. and Mikelsaar, M., 2004. Inhibition of clostridium difficile strains by intestinal Lactobacillus species. Journal of Medical Microbiology 53: 551-554.
'Inhibition of clostridium difficile strains by intestinal Lactobacillus species ' () 53 Journal of Medical Microbiology : 551 -554.
Rajilic-Stojanovic, M., Smidt, H. and De Vos, W.M., 2007. Diversity of the human gastrointestinal tract microbiota revisited. Environmental Microbiology 9: 2125-2136.
'Diversity of the human gastrointestinal tract microbiota revisited ' () 9 Environmental Microbiology : 2125 -2136.
Raoult, D., 2008. Obesity pandemics and the modification of digestive bacterial flora. European Journal of Clinical Microbiology and Infectious Diseases 27: 631-634.
'Obesity pandemics and the modification of digestive bacterial flora ' () 27 European Journal of Clinical Microbiology and Infectious Diseases : 631 -634.
Raoult, D., 2009. Probiotics and obesity: a link? Nature Reviews Microbiology 7: 616.
'Probiotics and obesity: a link? ' () 7 Nature Reviews Microbiology : 616.
Salzman, N.H., De Jong, H., Paterson, Y., Harmsen, H.J.M., Welling, G.W. and Bos, N.A., 2002. Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148: 3651-3660.
'Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria ' () 148 Microbiology : 3651 -3660.
Samuel, B.S. and Gordon, J.I., 2006. A humanized gnotobiotic mouse model of host archaeal-bacterial mutualism. Proceedings of the National Academy of Sciences of the USA 103: 10011-10016.
'A humanized gnotobiotic mouse model of host archaeal-bacterial mutualism ' () 103 Proceedings of the National Academy of Sciences of the USA : 10011 -10016.
Smith, C.J. and Osborn, A.M., 2008. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiology Ecology 67: 6-20.
'Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology ' () 67 FEMS Microbiology Ecology : 6 -20.
Song, Y., Liu, C. and Finegold, S.M., 2004. Real-time PCR quantitation of clostridia in feces of autistic children. Applied and Environmental Microbiology 70: 6459-6465.
'Real-time PCR quantitation of clostridia in feces of autistic children ' () 70 Applied and Environmental Microbiology : 6459 -6465.
Steer, T., Carpenter, H., Tuohy, K. and Gibson, G.R., 2000. Perspectives on the role of the human gut microbiota and its modulation by pro-and prebiotics. Nutrition Research Reviews 13: 229-254.
'Perspectives on the role of the human gut microbiota and its modulation by pro-and prebiotics ' () 13 Nutrition Research Reviews : 229 -254.
Sutter, V.L., Citron, D.M., Edelstein, M.A.C. and Finegold, S.M., 1985. Wadsworth anaerobic bacteriology manual. 4th ed. Star Publication, Belmont, CA, USA.
Wadsworth anaerobic bacteriology manual
Tappy, L. and Lê, K.-L., 2010. Metabolic effects of fructose and the worldwide increase in obesity. Physiological Reviews 90: 23-46.
'Metabolic effects of fructose and the worldwide increase in obesity ' () 90 Physiological Reviews : 23 -46.
Thomas III, A.B., Martha, D.W., Kathryn, K., Janet, K.S. and Lawrence, L.R., 2007. Monounsaturated fatty acyl-coenzyme A is predictive of atherosclerosis in human apoB-100 transgenic, LDLr-/-mice. Journal of Lipid Research 48: 1122-1131.
'Monounsaturated fatty acyl-coenzyme A is predictive of atherosclerosis in human apoB-100 transgenic, LDLr-/-mice ' () 48 Journal of Lipid Research : 1122 -1131.
Turnbaugh, P.J., Bäckhed, F., Fulton, L. and Gordon, J.I., 2008. Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host and Microbe 3: 213-223.
'Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome ' () 3 Cell Host and Microbe : 213 -223.
Turnbaugh, P.J., Hamady, M., Yatsunenko, T., Cantarel, B.L., Duncan, A., Ley, R.E., Sogin, M.L., Jones, W.J., Roe, B.A., Affourtit, J.P., Egholm, M., Henrissat, B., Heath, A.C., Knigh, R. and Gordon, J.I., 2009. A core gut microbiome in obese and lean twins. Nature 457: 480-484.
'A core gut microbiome in obese and lean twins ' () 457 Nature : 480 -484.
Turnbaugh, P.J., Ley, R.E., Mahowald, M.A., Magrini, V., Mardis, E.R. and Gordon, J.I., 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444: 1027-1031.
'An obesity-associated gut microbiome with increased capacity for energy harvest ' () 444 Nature : 1027 -1031.
Venema, K., 2010. Role of gut microbiota in the control of energy and carbohydrate metabolism. Current Opinion in Clinical Nutrition and Metabolic Care 12: 432-438.
'Role of gut microbiota in the control of energy and carbohydrate metabolism ' () 12 Current Opinion in Clinical Nutrition and Metabolic Care : 432 -438.
Wang, X., Brown, I.L., Evans, A.J. and Conway, P.L., 1999. The protective effects of high amylose maize (amylomaize) starch granules on the survival of Bifidobacterium spp. in the mouse intestinal tract. Journal of Applied Microbiology 87: 631-639.
'The protective effects of high amylose maize (amylomaize) starch granules on the survival of Bifidobacterium spp ' () 87 in the mouse intestinal tract. Journal of Applied Microbiology : 631 -639.
Xu, J., Bjursell, M.K., Himrod, J., Deng, S., Carmichael, L.K., Chiang, H.C., Hooper, L.V., and Gordon, J.I., 2003. A genomic view of the human-Bacteroides thetaiotaomicron symbiosis. Science 299: 2074-2076.
'A genomic view of the human-Bacteroides thetaiotaomicron symbiosis ' () 299 Science : 2074 -2076.
Yasutomi, K., Shinji, M., Takayoshi, S., Fumiko, A., Sayaka, K., Satoshi, S., Aki, K., Hiroyuki, A., Terry, G.U., Osamu, E. and Yoshihiro, O., 2008. Regulation of SREBP1c gene expression in skeletal muscle: role of retinoid X receptor/liver X receptor and forkhead-O1 transcription factor. Endocrinology 149: 2293-2305.
'Regulation of SREBP1c gene expression in skeletal muscle: role of retinoid X receptor/liver X receptor and forkhead-O1 transcription factor ' () 149 Endocrinology : 2293 -2305.
Yu, Z. and Morrison, M., 2004. Improved extraction of PCR-quality community DNA from digesta and fecal samples. BioTechniques 36: 808-812.
'Improved extraction of PCR-quality community DNA from digesta and fecal samples ' () 36 BioTechniques : 808 -812.
Zhang, H., DiBaise, J.K., Zuccolo, A., Kudrna, D., Braidotti, M., Yu, Y., Parameswaran, P., Crowell, M.D., Wing, R., Rittmann, B.E. and Krajmalnik-Brown, R., 2009. Human gut microbiota in obesity and after gastric bypass. Proceedings of the National Academy of Sciences of the USA 106: 2365-2370.
'Human gut microbiota in obesity and after gastric bypass ' () 106 Proceedings of the National Academy of Sciences of the USA : 2365 -2370.
Zhao, L., Xu, W., Ibrahim, S.A., Jin, J., Feng, J., Jiang, J., Meng, J. and Ren, F., 2011. Effects of age and region on fecal microflora in elderly subjects living in Bama, Guangxi, China. Current Microbiology 62: 64-70.
'Effects of age and region on fecal microflora in elderly subjects living in Bama, Guangxi, China ' () 62 Current Microbiology : 64 -70.
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The microbiota of the gastrointestinal tract (GIT) constitutes the major part of the total human microbiome and is considered to be an important regulator of human health and host metabolism. Numerous investigations in recent years have focused on the connection between the human microbiota and metabolic diseases such as obesity, type II diabetes and atherosclerosis. Yet, little is known about the impact of probiotic consumption on the GIT microbial population and the potential effect on chronic diseases. In this study, the modulation of the microbial community in the murine small intestine resulting from probiotic feeding was investigated and was found to be associated with an anti-obesity effect. Changes in the microbiota of the mouse faeces and small intestine were monitored using quantitative real-time PCR and by following the mRNA expression levels of various obesity-related biomarkers following probiotic feeding in a mouse model. Lactobacillus rhamnosus GG and Lactobacillus sakei NR28 (a putative probiotic strain isolated from kimchi) were administered at a daily level of approximately 1×108 viable bacteria per mouse (C57BL/6J mice) for up to three weeks. Feeding these strains resulted in a significant reduction of epididymal fat mass, as well as obesity-related biomarkers like acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase-1 in the liver. The total number and ratio of the microbial groups, i.e. Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp. were modulated in the small intestine, and the Firmicutes:Bacteroidetes ratio was decreased. In contrast, no noticeable effect of probiotic feeding could be detected on the faecal microbiota, neither quantitatively, nor with regard to the bacterial groups (Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp.) studied.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 46 | 0 | 0 |
Full Text Views | 414 | 362 | 59 |
PDF Views & Downloads | 318 | 264 | 19 |