Save

Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28

In: Beneficial Microbes
Authors:
Y.S Ji 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by Y.S Ji in
Current site
Google Scholar
PubMed
Close
,
H.N Kim 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by H.N Kim in
Current site
Google Scholar
PubMed
Close
,
H.J Park 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by H.J Park in
Current site
Google Scholar
PubMed
Close
,
J.E Lee 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by J.E Lee in
Current site
Google Scholar
PubMed
Close
,
S.Y Yeo 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by S.Y Yeo in
Current site
Google Scholar
PubMed
Close
,
J.S Yang 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by J.S Yang in
Current site
Google Scholar
PubMed
Close
,
S.Y Park 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by S.Y Park in
Current site
Google Scholar
PubMed
Close
,
H.S Yoon 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by H.S Yoon in
Current site
Google Scholar
PubMed
Close
,
G.S Cho 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by G.S Cho in
Current site
Google Scholar
PubMed
Close
,
C.M.A.P Franz 2Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institute, Haid-und-Neu-Straβe 9, 76131 Karlsruhe, Germany

Search for other papers by C.M.A.P Franz in
Current site
Google Scholar
PubMed
Close
,
A. Bomba 3Faculty of Medicine, Institute of Experimental Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovakia

Search for other papers by A. Bomba in
Current site
Google Scholar
PubMed
Close
,
H.K Shin 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea

Search for other papers by H.K Shin in
Current site
Google Scholar
PubMed
Close
, and
W.H Holzapfel 1School of Life Science, Handong Global University, Pohang, Gyungbuk 791-708, Republic of Korea
wilhelm@woodapple.net

Search for other papers by W.H Holzapfel in
Current site
Google Scholar
PubMed
Close
Open Access

Abstract

The microbiota of the gastrointestinal tract (GIT) constitutes the major part of the total human microbiome and is considered to be an important regulator of human health and host metabolism. Numerous investigations in recent years have focused on the connection between the human microbiota and metabolic diseases such as obesity, type II diabetes and atherosclerosis. Yet, little is known about the impact of probiotic consumption on the GIT microbial population and the potential effect on chronic diseases. In this study, the modulation of the microbial community in the murine small intestine resulting from probiotic feeding was investigated and was found to be associated with an anti-obesity effect. Changes in the microbiota of the mouse faeces and small intestine were monitored using quantitative real-time PCR and by following the mRNA expression levels of various obesity-related biomarkers following probiotic feeding in a mouse model. Lactobacillus rhamnosus GG and Lactobacillus sakei NR28 (a putative probiotic strain isolated from kimchi) were administered at a daily level of approximately 1×108 viable bacteria per mouse (C57BL/6J mice) for up to three weeks. Feeding these strains resulted in a significant reduction of epididymal fat mass, as well as obesity-related biomarkers like acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase-1 in the liver. The total number and ratio of the microbial groups, i.e. Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp. were modulated in the small intestine, and the Firmicutes:Bacteroidetes ratio was decreased. In contrast, no noticeable effect of probiotic feeding could be detected on the faecal microbiota, neither quantitatively, nor with regard to the bacterial groups (Firmicutes, Bacteroidetes, Clostridium cluster I and XIVab, and Lactobacillus spp.) studied.

Content Metrics

All Time Past 365 days Past 30 Days
Abstract Views 46 0 0
Full Text Views 414 362 59
PDF Views & Downloads 318 264 19