Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals
In: Beneficial MicrobesSearch for other papers by N.J. Hepburn in
Current site
Google Scholar
Search for other papers by I. Garaiova in
Current site
Google Scholar
Search for other papers by E.A. Williams in
Current site
Google Scholar
Search for other papers by D.R. Michael in
Current site
Google Scholar
Search for other papers by S. Plummer in
Current site
Google Scholar
The objective of this study was to examine the effect of daily probiotic supplementation upon the immune profile of healthy participants by the assessment of ex vivo cytokine production. Twenty healthy adult volunteers received a multi-strain probiotic supplement consisting of two strains of Lactobacillus acidophilus (CUL60 and CUL21), Bifidobacterium lactis (CUL34) and Bifidobacterium bifidum (CUL20) and fructooligosaccharide for 12 weeks. Blood samples were collected at baseline, 6 and 12 weeks. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured ex vivo in the presence or absence of lipopolysaccharide and cytokine production was assessed. Postintervention, a significant decrease in the production of interleukin-6 and interleukin-1β was apparent when PBMCs were incubated in the presence of lipopolysaccharide, whilst a significant increase in IL-10 and transforning growth factor-β production was seen when the cells were incubated without an additional stimulus. This preliminary study demonstrates the potential of a multi-strain probiotic supplement to alter the immune response as demonstrated by changes in ex vivo cytokine production. Such results demonstrate the potential benefit of probiotic supplementation for healthy individuals and warrants further investigation.
-
Avadhani, A. and Miley, H., 2011. Probiotics for prevention of antibiotic-associated diarrhea and Clostridium difficile-associated disease in hospitalized adults - a meta-analysis. Journal of the American Academy of Nurse Practitioners 23: 269-274.
'Probiotics for prevention of antibiotic-associated diarrhea and Clostridium difficile-associated disease in hospitalized adults - a meta-analysis ' () 23 Journal of the American Academy of Nurse Practitioners : 269 -274.
-
Boirivant, M. and Strober, W., 2007. The mechanism of action of probiotics. Current Opinion in Gastroenterology 23: 679-692.
'The mechanism of action of probiotics ' () 23 Current Opinion in Gastroenterology : 679 -692.
-
Bouhnik, Y., Achour, L., Paineau, D., Riottot, M., Attar, A. and Bornet, F., 2007. Four-week short chain fructo-oligosaccharides ingestion leads to increasing fecal bifidobacteria and cholesterol excretion in healthy elderly volunteers. Nutrition Journal 6: 42.
'Four-week short chain fructo-oligosaccharides ingestion leads to increasing fecal bifidobacteria and cholesterol excretion in healthy elderly volunteers ' () 6 Nutrition Journal : 42.
-
Briet, F., Achour, L., Flourié, B., Beaugerie, L., Pellier, P., Franchisseur, C., Bornet, F. and Rambaud, J.C., 1995. Symptomatic response to varying levels of fructo-oligosaccharides consumed occasionally or regularly. European Journal of Clinical Nutrition 49: 501-507.
'Symptomatic response to varying levels of fructo-oligosaccharides consumed occasionally or regularly ' () 49 European Journal of Clinical Nutrition : 501 -507.
-
Chapman, C.M., Gibson, G.R. and Rowland, I., 2011. Health benefits of probiotics: are mixtures more effective than single strains? European Journal of Nutrition 50: 1-17.
'Health benefits of probiotics: are mixtures more effective than single strains? ' () 50 European Journal of Nutrition : 1 -17.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Available at: http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf. http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf
-
González-Hernández, L.A., Jave-Suarez, L.F., Fafutis-Morris, M., Montes-Salcedo, K.E., Valle-Gutierrez, L.G., Campos-Loza, A.E., Enciso-Gómez, L.F. and Andrade-Villanueva, J.F., 2012. Synbiotic therapy decreases microbial translocation and inflammation and improves immunological status in HIV-infected patients: a double-blind randomized controlled pilot trial. Nutrition Journal 11: 90.
'Synbiotic therapy decreases microbial translocation and inflammation and improves immunological status in HIV-infected patients: a double-blind randomized controlled pilot trial ' () 11 Nutrition Journal : 90.
-
Kekkonen, R.A., Lummela, N., Karjalainen, H., Latvala, S., Tynkkynen, S., Jarvenpaa, S., Kautiainen, H., Julkunen, I., Vapaatalo, H. and Korpela, R., 2008. Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults. World Journal of Gastroenterology 14: 2029-2036.
'Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults ' () 14 World Journal of Gastroenterology : 2029 -2036.
-
Kwon, H.K., Lee, C.G., So, J.S., Chae, C.S., Hwang, J.S., Sahoo, A., Nam, J.H., Rhee, J.H., Hwang, K.C. and Im, S.H., 2010. Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders. Proceedings of the National Academy of Sciences of the USA 107: 2159-2164.
'Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders ' () 107 Proceedings of the National Academy of Sciences of the USA : 2159 -2164.
-
Lindsay, J.O., Whelan, K., Stagg, A.J., Gobin, P., Al-Hassi, H.O., Rayment, N., Kamm, M.A., Knight, S.C. and Forbes, A., 2006. Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease. Gut 55: 348-355.
'Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease ' () 55 Gut : 348 -355.
-
Mangalat, N., Liu, Y., Fatheree, N.Y., Ferris, M.J., Van Arsdall, M.R., Chen, Z., Rahbar, M.H., Gleason, W.A., Norori, J., Tran, D.Q. and Rhoads, J.M., 2012. Safety and tolerability of Lactobacillus reuteri DSM 17938 and effects on biomarkers in healthy adults: results from a randomized masked trial. PloS ONE 7: e43910.
'Safety and tolerability of Lactobacillus reuteri DSM 17938 and effects on biomarkers in healthy adults: results from a randomized masked trial ' () 7 PloS ONE : e43910.
-
Pelucchi, C., Chatenoud, L., Turati, F., Galeone, C., Moja, L., Bach, J.F. and La Vecchia, C., 2012. Probiotics supplementation during pregnancy or infancy for the prevention of atopic dermatitis: a meta-analysis. Epidemiology 23: 402-414.
'Probiotics supplementation during pregnancy or infancy for the prevention of atopic dermatitis: a meta-analysis ' () 23 Epidemiology : 402 -414.
-
Ritchie, M.L. and Romanuk, T.N., 2012. A meta-analysis of probiotic efficacy for gastrointestinal diseases. PloS ONE 7: e34938.
'A meta-analysis of probiotic efficacy for gastrointestinal diseases ' () 7 PloS ONE : e34938.
-
Rizzardini, G., Eskesen, D., Calder, P.C., Capetti, A., Jespersen, L. and Clerici, M., 2012. Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. British Journal of Nutrition 107: 876-884.
'Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study ' () 107 British Journal of Nutrition : 876 -884.
-
Rizzello, V., Bonaccorsi, I., Dongarrà, M.L., Fink, L.N. and Ferlazzo, G., 2011. Role of natural killer and dendritic cell crosstalk in immunomodulation by commensal bacteria probiotics. Journal of Biomedicine and Biotechnology 2011: 473097.
'Role of natural killer and dendritic cell crosstalk in immunomodulation by commensal bacteria probiotics ' () 2011 Journal of Biomedicine and Biotechnology : 473097.
-
Roberfroid, M., Gibson, G.R., Hoyles, L., McCartney, A.L., Rastall, R., Rowland, I., Wolvers, D., Watzl, B., Szajewska, H., Stahl, B., Guarner, F., Respondek, F., Whelan, K., Coxam, V., Davicco, M.J., Léotoing, L., Wittrant, Y., Delzenne, N.M., Cani, P.D., Neyrinck, A.M. and Meheust, A., 2010. Prebiotic effects: metabolic and health benefits. British Journal of Nutrition 104 Suppl. 2: S1-S63.
'Prebiotic effects: metabolic and health benefits ' () 104 British Journal of Nutrition : S1 -S63.
-
Sheih, Y.H., Chiang, B.L., Wang, L.H., Liao, C.K. and Gill, H.S., 2001. Systemic immunity-enhancing effects in healthy subjects following dietary consumption of the lactic acid bacterium Lactobacillus rhamnosus HN001. Journal of the American College of Nutrition 20: 149-156.
'Systemic immunity-enhancing effects in healthy subjects following dietary consumption of the lactic acid bacterium Lactobacillus rhamnosus HN001 ' () 20 Journal of the American College of Nutrition : 149 -156.
-
Smits, H.H., Engering, A., Van der Kleij, D., De Jong, E.C., Schipper, K., Van Capel, T.M., Zaat, B.A., Yazdanbakhsh, M., Wierenga, E.A., Van Kooyk, Y. and Kapsenberg, M.L., 2005. Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin. Journal of Allergy and Clinical Immunology 115: 1260-1267.
'Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin ' () 115 Journal of Allergy and Clinical Immunology : 1260 -1267.
-
Ten Bruggencate, S.J., Bovee-Oudenhoven, I.M., Lettink-Wissink, M.L., Katan, M.B. and Van der Meer, R., 2006. Dietary fructooligosaccharides affect intestinal barrier function in healthy men. Journal of Nutrition 136: 70-74.
'Dietary fructooligosaccharides affect intestinal barrier function in healthy men ' () 136 Journal of Nutrition : 70 -74.
-
Vanderpool, C., Yan, F. and Polk, D.B., 2008. Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases. Inflammatory Bowel Diseases 14: 1585-1596.
'Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases ' () 14 Inflammatory Bowel Diseases : 1585 -1596.
-
Williams, E.A., Stimpson, J., Wang, D., Plummer, S., Garaiova, I., Barker, M.E. and Corfe, B.M., 2009. Clinical trial: a multistrain probiotic preparation significantly reduces symptoms of irritable bowel syndrome in a double-blind placebo-controlled study. Alimentary Pharmacology and Therapeutics 29: 97-103.
'Clinical trial: a multistrain probiotic preparation significantly reduces symptoms of irritable bowel syndrome in a double-blind placebo-controlled study ' () 29 Alimentary Pharmacology and Therapeutics : 97 -103.
-
Zenhom, M., Hyder, A., De Vrese, M., Heller, K.J., Roeder, T. and Schrezenmeir, J., 2011. Prebiotic oligosaccharides reduce proinflammatory cytokines in intestinal Caco-2 cells via activation of PPARγ and peptidoglycan recognition protein 3. Journal of Nutrition 141: 971-977.
'Prebiotic oligosaccharides reduce proinflammatory cytokines in intestinal Caco-2 cells via activation of PPARγ and peptidoglycan recognition protein 3 ' () 141 Journal of Nutrition : 971 -977.
Supplementary Materials
Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals
In: Beneficial MicrobesSearch for other papers by N.J. Hepburn in
Current site
Google Scholar
PubMed
Search for other papers by I. Garaiova in
Current site
Google Scholar
PubMed
Search for other papers by E.A. Williams in
Current site
Google Scholar
PubMed
Search for other papers by D.R. Michael in
Current site
Google Scholar
PubMed
Search for other papers by S. Plummer in
Current site
Google Scholar
PubMed
The objective of this study was to examine the effect of daily probiotic supplementation upon the immune profile of healthy participants by the assessment of ex vivo cytokine production. Twenty healthy adult volunteers received a multi-strain probiotic supplement consisting of two strains of Lactobacillus acidophilus (CUL60 and CUL21), Bifidobacterium lactis (CUL34) and Bifidobacterium bifidum (CUL20) and fructooligosaccharide for 12 weeks. Blood samples were collected at baseline, 6 and 12 weeks. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured ex vivo in the presence or absence of lipopolysaccharide and cytokine production was assessed. Postintervention, a significant decrease in the production of interleukin-6 and interleukin-1β was apparent when PBMCs were incubated in the presence of lipopolysaccharide, whilst a significant increase in IL-10 and transforning growth factor-β production was seen when the cells were incubated without an additional stimulus. This preliminary study demonstrates the potential of a multi-strain probiotic supplement to alter the immune response as demonstrated by changes in ex vivo cytokine production. Such results demonstrate the potential benefit of probiotic supplementation for healthy individuals and warrants further investigation.