Indigenous microbiota plays a crucial role in the development of several intestinal diseases, including mucositis. Gastrointestinal mucositis is a major and serious side effect of cancer therapy, and there is no effective therapy for this clinical condition. However, some probiotics have been shown to attenuate such conditions. To evaluate the effects of Saccharomyces cerevisiae UFMG A-905 (Sc-905), a potential probiotic yeast, we investigated whether pre- or post-treatment with viable or inactivated Sc-905 could prevent weight loss and intestinal lesions, and maintain integrity of the mucosal barrier in a mucositis model induced by irinotecan in mice. Only post-treatment with viable Sc-905 was able to protect mice against the damage caused by chemotherapy, reducing the weight loss, increase of intestinal permeability and jejunal lesions (villous shortening). Besides, this treatment reduced oxidative stress, prevented the decrease of goblet cells and stimulated the replication of cells in the intestinal crypts of mice with experimental mucositis. In conclusion, Sc-905 protects animals against irinotecan-induced mucositis when administered as a post-treatment with viable cells, and this effect seems to be related with the reduction of oxidative stress and preservation of intestinal mucosa.
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Al-Dasooqi, N., Bowen, J.M., Gibson, R.J., Logan, R.M., Stringer, A.M. and Keefe, D.M., 2011. Irinotecan-induced alterations in intestinal cell kinetics and extracellular matrix component expression in the Dark Agouti rat. International Journal of Experimental Pathology 92: 357-365.
'Irinotecan-induced alterations in intestinal cell kinetics and extracellular matrix component expression in the Dark Agouti rat ' () 92 International Journal of Experimental Pathology : 357 -365.
Alimonti, A., Gelibter, A., Pavese, I., Satta, F., Cognetti, F., Ferretti, G., Rasio, D., Vecchione, A. and Di Palma, M., 2004. New approaches to prevent intestinal toxicity of irinotecan-based regimens. Cancer Treatment Reviews 30: 555-562.
'New approaches to prevent intestinal toxicity of irinotecan-based regimens ' () 30 Cancer Treatment Reviews : 555 -562.
Arifa, R.D., Madeira, M.F., de Paula, T.P., Lima, R.L., Tavares, L.D., Menezes-Garcia, Z., Fagundes, C.T., Rachid, M.A., Ryffel, B., Zamboni, D.S., Teixeira, M.M. and Souza, D.G., 2014. Inflammasome activation is reactive oxygen species dependent and mediates irinotecan-induced mucositis through IL-1β and IL-18 in mice. American Journal of Pathology 184: 2023-2034.
'Inflammasome activation is reactive oxygen species dependent and mediates irinotecan-induced mucositis through IL-1β and IL-18 in mice ' () 184 American Journal of Pathology : 2023 -2034.
Bowen, J.M., Stringer, A.M., Gibson, R.J., Yeoh, A.S., Hannam, S. and Keefe, D.M., 2007. VSL#3 probiotic treatment reduces chemotherapy-induced diarrhea and weight loss. Cancer Biology & Therapy 6: 1449-1454.
'VSL#3 probiotic treatment reduces chemotherapy-induced diarrhea and weight loss ' () 6 Cancer Biology & Therapy : 1449 -1454.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ' () 72 Analytical Biochemistry : 248 -254.
Brandi, G., Dabard, J., Raibaud, P., Di Battista, M., Bridonneau, C., Pisi, A.M., Morselli Labate, A.M., Pantaleo, M.A., De Vivo, A. and Biasco, G., 2006. Intestinal microflora and digestive toxicity of irinotecan in mice. Clinical Cancer Research 12: 1299-1307.
'Intestinal microflora and digestive toxicity of irinotecan in mice ' () 12 Clinical Cancer Research : 1299 -1307.
Brestoff, J.R. and Artis, D., 2013. Commensal bacteria at the interface of host metabolism and the immune system. Nature Immunology 14: 676-684.
'Commensal bacteria at the interface of host metabolism and the immune system ' () 14 Nature Immunology : 676 -684.
Buccigrossi, V., Laudiero, G., Russo, C., Miele, E., Sofia, M., Monini, M., Ruggeri, F.M. and Guarino, A., 2014. Chloride secretion induced by rotavirus is oxidative stress-dependent and inhibited by Saccharomyces boulardii in human enterocytes. PloS One 9: e99830.
'Chloride secretion induced by rotavirus is oxidative stress-dependent and inhibited by Saccharomyces boulardii in human enterocytes ' () 9 PloS One : e99830.
Cesaro, S., Chinello, P., Rossi, L. and Zanesco, L., 2000. Saccharomyces cerevisiae fungemia in a neutropenic patient treated with Saccharomyces boulardii. Supportive Care in Cancer 8: 504-505.
'Saccharomyces cerevisiae fungemia in a neutropenic patient treated with Saccharomyces boulardii ' () 8 Supportive Care in Cancer : 504 -505.
Chen, S., Yueh, M.F., Bigo, C., Barbier, O., Wang, K., Karin, M., Nguyen, N. and Tukey, R.H., 2013. Intestinal glucuronidation protects against chemotherapy-induced toxicity by irinotecan (CPT-11). Proceedings of the National Academy of Sciences of the USA 110: 19143-19148.
Intestinal glucuronidation protects against chemotherapy-induced toxicity by irinotecan (CPT-11) Proceedings of the National Academy of Sciences of the USA 110 19143 19148
De Freitas Cuba, L., Salum, F.G., Cherubini, K. and de Figueiredo, M.A., 2015. Antioxidant agents: a future alternative approach in the prevention and treatment of radiation-induced oral mucositis? Alternative Therapies in Health and Medicine 21: 36-41.
'Antioxidant agents: a future alternative approach in the prevention and treatment of radiation-induced oral mucositis? ' () 21 Alternative Therapies in Health and Medicine : 36 -41.
Draper, H.H. and Hadley, M., 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 186: 421-431.
'Malondialdehyde determination as index of lipid peroxidation ' () 186 Methods in Enzymology : 421 -431.
Duman, D.G., Kumral, Z.N., Ercan, F., Deniz, M., Can, G. and Cağlayan Yeğen, B., 2013. Saccharomyces boulardii ameliorates clarithromycin- and methotrexate-induced intestinal and hepatic injury in rats. British Journal of Nutrition 110: 493-499.
'Saccharomyces boulardii ameliorates clarithromycin- and methotrexate-induced intestinal and hepatic injury in rats ' () 110 British Journal of Nutrition : 493 -499.
Ellman, G.L., 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82: 70-77.
'Tissue sulfhydryl groups ' () 82 Archives of Biochemistry and Biophysics : 70 -77.
Flieger, D., Klassert, C., Hainke, S., Keller, R., Kleinschmidt, R. and Fischbach, W., 2007. Phase II clinical trial for prevention of delayed diarrhea with cholestyramine/levofloxacin in the second-line treatment with irinotecan biweekly in patients with metastatic colorectal carcinoma. Oncology 72: 10-16.
'Phase II clinical trial for prevention of delayed diarrhea with cholestyramine/levofloxacin in the second-line treatment with irinotecan biweekly in patients with metastatic colorectal carcinoma ' () 72 Oncology : 10 -16.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on draftingguidelines for the evaluation of probiotics in food. Available at: ftp://ftp.fao.org/es/esn/food/wgreport2.pdf.
Generoso, S.V., Viana, M., Santos, R., Martins, F.S., Machado, J.A., Arantes, R.M., Nicoli, J.R., Correia, M.I. and Cardoso, V.N., 2010. Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model. Archives of Microbiology 192: 477-484.
'Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model ' () 192 Archives of Microbiology : 477 -484.
Gibson, R.J. and Stringer, A.M., 2009. Chemotherapy-induced diarrhoea. Current Opinion Support Palliat Care 3: 31-35.
'Chemotherapy-induced diarrhoea ' () 3 Current Opinion Support Palliat Care : 31 -35.
Gibson, R.J., Bowen, J.M., Inglis, M.R., Cummins, A.G. and Keefe, D.M., 2003. Irinotecan causes severe small intestinal damage, as well as colonic damage, in the rat with implanted breast cancer. Journal of Gastroenterology and Hepatology 18: 1095-1100.
'Irinotecan causes severe small intestinal damage, as well as colonic damage, in the rat with implanted breast cancer ' () 18 Journal of Gastroenterology and Hepatology : 1095 -1100.
Ishii, Y., Sugimoto, S., Izawa, N., Sone, T., Chiba, K. and Miyazaki, K., 2014. Oral administration of Bifidobacterium breve attenuates UV-induced barrier perturbation and oxidative stress in hairless mice skin. Archives of Dermatological Research 306: 467-473.
'Oral administration of Bifidobacterium breve attenuates UV-induced barrier perturbation and oxidative stress in hairless mice skin ' () 306 Archives of Dermatological Research : 467 -473.
Javle, M.M., Cao, S., Durrani, F.A., Pendyala, L., Lawrence, D.D., Smith, P.F., Creaven, P.J., Noel, D.C., Iyer, R.V. and Rustum, Y.M., 2007. Celecoxib and mucosal protection: translation from an animal model to a phase I clinical trial of celecoxib, irinotecan, and 5-fluorouracil. Clinical Cancer Research 13: 965-971.
'Celecoxib and mucosal protection: translation from an animal model to a phase I clinical trial of celecoxib, irinotecan, and 5-fluorouracil ' () 13 Clinical Cancer Research : 965 -971.
Justino, P.F., Melo, L.F., Nogueira, A.F., Costa, J.V., Silva, L.M., Santos, C.M., Mendes, W.O., Costa, M.R., Franco, A.X., Lima, A.A., Ribeiro, R.A., Souza, M.H. and Soares, P.M., 2014. Treatment with Saccharomyces boulardii reduces the inflammation and dysfunction of the gastrointestinal tract in 5-fluorouracil-induced intestinal mucositis in mice. British Journal of Nutrition 111: 1611-1621.
'Treatment with Saccharomyces boulardii reduces the inflammation and dysfunction of the gastrointestinal tract in 5-fluorouracil-induced intestinal mucositis in mice ' () 111 British Journal of Nutrition : 1611 -1621.
Kaushal, D. and Kansal, V.K., 2012. Probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum alleviates age-inflicted oxidative stress and improves expression of biomarkers of ageing in mice. Molecular Biology Reports 39: 1791-1799.
'Probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum alleviates age-inflicted oxidative stress and improves expression of biomarkers of ageing in mice ' () 39 Molecular Biology Reports : 1791 -1799.
Kehrer, D.F., Sparreboom, A., Verweij, J., de Bruijn, P., Nierop, C.A., van de Schraaf, J., Ruijgrok, E.J. and De Jonge, M.J., 2001. Modulation of irinotecan-induced diarrhea by cotreatment with neomycin in cancer patients. Clinical Cancer Research 7: 1136-1141.
'Modulation of irinotecan-induced diarrhea by cotreatment with neomycin in cancer patients ' () 7 Clinical Cancer Research : 1136 -1141.
Kweekel, D., Guchelaar, H.J. and Gelderblom, H., 2008. Clinical and pharmacogenetic factors associated with irinotecan toxicity. Cancer Treatment Reviews 34: 656-669.
'Clinical and pharmacogenetic factors associated with irinotecan toxicity ' () 34 Cancer Treatment Reviews : 656 -669.
Liong, M.T., 2008. Safety of probiotics: translocation and infection. Nutrition Reviews 66: 192-202.
'Safety of probiotics: translocation and infection ' () 66 Nutrition Reviews : 192 -202.
Logan, R.M., Stringer, A.M., Bowen, J.M., Yeoh, A.S., Gibson, R.J., Sonis, S.T. and Keefe, D.M., 2007. The role of pro-inflammatory cytokines in cancer treatment-induced alimentary tract mucositis: pathobiology, animal models and cytotoxic drugs. Cancer Treatment Reviews 33: 448-460.
'The role of pro-inflammatory cytokines in cancer treatment-induced alimentary tract mucositis: pathobiology, animal models and cytotoxic drugs ' () 33 Cancer Treatment Reviews : 448 -460.
Maioli, T.U., De Melo Silva, B., Dias, M.N., Paiva, N.C., Cardoso, V.N., Fernandes, S.O., Carneiro, C.M., Dos Santos Martins, F. and De Vasconcelos Generoso, S., 2014. Pretreatment with Saccharomyces boulardii does not prevent the experimental mucositis in Swiss mice. Journal of Negative Results in Biomedicine 13: 6.
'Pretreatment with Saccharomyces boulardii does not prevent the experimental mucositis in Swiss mice ' () 13 Journal of Negative Results in Biomedicine : 6.
Martins, F.S., Elian, S.D., Vieira, A.T., Tiago, F.C., Martins, A.K., Silva, F.C., Souza, E.L., Sousa, L.P., Araújo, H.R., Pimenta, P.F., Bonjardim, C.A., Arantes, R.M., Teixeira, M.M. and Nicoli, J.R., 2011. Oral treatment with Saccharomyces cerevisiae strain UFMG 905 modulates immune responses and interferes with signal pathways involved in the activation of inflammation in a murine model of typhoid fever. International Journal of Medical Microbiology 301: 359-364.
'Oral treatment with Saccharomyces cerevisiae strain UFMG 905 modulates immune responses and interferes with signal pathways involved in the activation of inflammation in a murine model of typhoid fever ' () 301 International Journal of Medical Microbiology : 359 -364.
Martins, F.S., Nardi, R.M., Arantes, R.M., Rosa, C.A., Neves, M.J. and Nicoli, J.R., 2005. Screening of yeasts as probiotic based on capacities to colonize the gastrointestinal tract and to protect against enteropathogen challenge in mice. Journal of General and Applied Microbiology 51: 83-92.
'Screening of yeasts as probiotic based on capacities to colonize the gastrointestinal tract and to protect against enteropathogen challenge in mice ' () 51 Journal of General and Applied Microbiology : 83 -92.
Martins, F.S., Rodrigues, A.C., Tiago, F.C., Penna, F.J., Rosa, C.A., Arantes, R.M., Nardi, R.M., Neves, M.J. and Nicoli, J.R., 2007. Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice. Journal of Medical Microbiology 56: 352-359.
'Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice ' () 56 Journal of Medical Microbiology : 352 -359.
Mishra, V., Shah, C., Mokashe, N., Chavan, R., Yadav, H. and Prajapati, J., 2015. Probiotics as potential antioxidants: a systematic review. Journal of Agricultural and Food Chemistry 63: 3615-3626.
'Probiotics as potential antioxidants: a systematic review ' () 63 Journal of Agricultural and Food Chemistry : 3615 -3626.
Pedroso, S.H., Vieira, A.T., Bastos, R.W., Oliveira, J.S., Cartelle, C.T., Arantes, R.E., Soares, P.G., Generoso, S.V., Cardoso, V.N., Teixeira, M.M., Nicoli, J.R. and Martins, F.S., 2015. Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice. Microbiology 161: 1950-1960.
'Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice ' () 161 Microbiology : 1950 -1960.
Pico, J.L., Avila-Garavito, A. and Naccache, P., 1998. Mucositis: Its Occurrence, Consequences, and Treatment in the Oncology Setting. Oncologist 3: 446-451.
'Mucositis: Its Occurrence, Consequences, and Treatment in the Oncology Setting ' () 3 Oncologist : 446 -451.
Pommier, Y., 2006. Topoisomerase I inhibitors: camptothecins and beyond. Nature Reviews Cancer 6: 789-802.
'Topoisomerase I inhibitors: camptothecins and beyond ' () 6 Nature Reviews Cancer : 789 -802.
Sezer, A., Usta, U. and Cicin, I., 2009. The effect of Saccharomyces boulardii on reducing irinotecan-induced intestinal mucositis and diarrhea. Medical Oncology 26: 350-357.
'The effect of Saccharomyces boulardii on reducing irinotecan-induced intestinal mucositis and diarrhea ' () 26 Medical Oncology : 350 -357.
Stringer, A.M., Gibson, R.J., Bowen, J.M., Logan, R.M., Ashton, K., Yeoh, A.S., Al-Dasooqi, N. and Keefe, D.M., 2009a. Irinotecan-induced mucositis manifesting as diarrhoea corresponds with an amended intestinal flora and mucin profile. International Journal of Experimental Pathology 90: 489-499.
'Irinotecan-induced mucositis manifesting as diarrhoea corresponds with an amended intestinal flora and mucin profile ' () 90 International Journal of Experimental Pathology : 489 -499.
Stringer, A.M., Gibson, R.J., Logan, R.M., Bowen, J.M., Yeoh, A.S., Laurence, J. and Keefe, D.M., 2009b. Irinotecan-induced mucositis is associated with changes in intestinal mucins. Cancer Chemotherapy and Pharmacology 64: 123-132.
'Irinotecan-induced mucositis is associated with changes in intestinal mucins ' () 64 Cancer Chemotherapy and Pharmacology : 123 -132.
Takasuna, K., Hagiwara, T., Hirohashi, M., Kato, M., Nomura, M., Nagai, E., Yokoi, T. and Kamataki, T., 1998. Inhibition of intestinal microflora beta-glucuronidase modifies the distribution of the active metabolite of the antitumor agent, irinotecan hydrochloride (CPT-11) in rats. Cancer Chemotherapy and Pharmacology 42: 280-286.
'Inhibition of intestinal microflora beta-glucuronidase modifies the distribution of the active metabolite of the antitumor agent, irinotecan hydrochloride (CPT-11) in rats ' () 42 Cancer Chemotherapy and Pharmacology : 280 -286.
Tiago, F.C., Martins, F.S., Souza, E.L., Pimenta, P.F., Araujo, H.R., Castro, I.M., Brandão, R.L. and Nicoli, J.R., 2012. Adhesion to the yeast cell surface as a mechanism for trapping pathogenic bacteria by Saccharomyces probiotics. Journal of Medical Microbiology 61: 1194-1207.
'Adhesion to the yeast cell surface as a mechanism for trapping pathogenic bacteria by Saccharomyces probiotics ' () 61 Journal of Medical Microbiology : 1194 -1207.
Tiago, F.C., Porto, B.A., Ribeiro, N.S., Moreira, L.M., Arantes, R.M., Vieira, A.T., Teixeira, M.M., Generoso, S.V., Nascimento, V.N., Martins, F.S. and Nicoli, J.R., 2015. Effect of Saccharomyces cerevisiae strain UFMG A-905 in experimental model of inflammatory bowel disease. Beneficial Microbes 6: 807-815.
'Effect of Saccharomyces cerevisiae strain UFMG A-905 in experimental model of inflammatory bowel disease ' () 6 Beneficial Microbes : 807 -815.
Vieira, A.T., Teixeira, M.M. and Martins, F.S., 2013. The role of probiotics and prebiotics in inducing gut immunity. Frontiers in Immunology 4: 445.
'The role of probiotics and prebiotics in inducing gut immunity ' () 4 Frontiers in Immunology : 445.
Wallace, B.D., Wang, H., Lane, K.T., Scott, J.E., Orans, J., Koo, J.S., Venkatesh, M., Jobin, C., Yeh, L.A., Mani, S. and Redinbo, M.R., 2010. Alleviating cancer drug toxicity by inhibiting a bacterial enzyme. Science 330: 831-835.
'Alleviating cancer drug toxicity by inhibiting a bacterial enzyme ' () 330 Science : 831 -835.
Wirtz, S., Neufert, C., Weigmann, B. and Neurath, M.F., 2007. Chemically induced mouse models of intestinal inflammation. Nature Protocols 2: 541-546.
'Chemically induced mouse models of intestinal inflammation ' () 2 Nature Protocols : 541 -546.
Zwielehner, J., Lassl, C., Hippe, B., Pointner, A., Switzeny, O.J., Remely, M., Kitzweger, E., Ruckser, R. and Haslberger, A.G., 2011. Changes in human fecal microbiota due to chemotherapy analyzed by TaqMan-PCR, 454 sequencing and PCR-DGGE fingerprinting. PloS One 6: e28654.
'Changes in human fecal microbiota due to chemotherapy analyzed by TaqMan-PCR, 454 sequencing and PCR-DGGE fingerprinting ' () 6 PloS One : e28654.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 380 | 210 | 39 |
| Full Text Views | 12 | 4 | 0 |
| PDF Views & Downloads | 6 | 5 | 0 |
Indigenous microbiota plays a crucial role in the development of several intestinal diseases, including mucositis. Gastrointestinal mucositis is a major and serious side effect of cancer therapy, and there is no effective therapy for this clinical condition. However, some probiotics have been shown to attenuate such conditions. To evaluate the effects of Saccharomyces cerevisiae UFMG A-905 (Sc-905), a potential probiotic yeast, we investigated whether pre- or post-treatment with viable or inactivated Sc-905 could prevent weight loss and intestinal lesions, and maintain integrity of the mucosal barrier in a mucositis model induced by irinotecan in mice. Only post-treatment with viable Sc-905 was able to protect mice against the damage caused by chemotherapy, reducing the weight loss, increase of intestinal permeability and jejunal lesions (villous shortening). Besides, this treatment reduced oxidative stress, prevented the decrease of goblet cells and stimulated the replication of cells in the intestinal crypts of mice with experimental mucositis. In conclusion, Sc-905 protects animals against irinotecan-induced mucositis when administered as a post-treatment with viable cells, and this effect seems to be related with the reduction of oxidative stress and preservation of intestinal mucosa.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 380 | 210 | 39 |
| Full Text Views | 12 | 4 | 0 |
| PDF Views & Downloads | 6 | 5 | 0 |