Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus.
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Laboratorio de Biotecnología, Facultad de Ingeniería-Universidad ORT Uruguay, Cuareim 1451, 11100 Montevideo, Uruguay.
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Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Calle 47 y 116, B1900AJI La Plata, Argentina.
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It is known that probiotic microorganisms are able to modulate pathogen virulence. This ability is strain dependent and involves multiple interactions between microorganisms and relevant host’s cell populations. In the present work we focus on the effect of a potentially probiotic lactobacillus strain (Lactobacillus delbrueckii subsp. lactis CIDCA 133) in an in vitro model of Bacillus cereus infection. Our results showed that infection of intestinal epithelial HT-29 cells by B. cereus induces nuclear factor kappa B (NF-κB) pathway. Noteworthy, the presence of strain L. delbrueckii subsp.lactis CIDCA 133 increases stimulation. However, B. cereus-induced interleukin (IL)-8 production by epithelial cells is partially abrogated by L. delbrueckii subsp. lactis CIDCA 133. These findings suggest that signalling pathways other than that of NF-κB are involved. In a co-culture system (HT-29 and monocyte-derived dendritic cells), B. cereus was able to translocate from the epithelial (upper) to the dendritic cell compartment (lower). This translocation was partially abrogated by the presence of lactobacilli in the upper compartment. In addition, infection of epithelial cells in the co-culture model, led to an increase in the expression of CD86 by dendritic cells. This effect could not be modified in the presence of lactobacilli. Interestingly, infection of enterocytes with B. cereus triggers production of proinflammatory cytokines by dendritic cells (IL-8, IL-6 and tumour necrosis factor alpha (TNF-α)). The production of TNF-α (a protective cytokine in B. cereus infections) by dendritic cells was increased in the presence of lactobacilli. The present work demonstrates for the first time the effect of L. delbrueckii subsp. lactis CIDCA 133, a potentially probiotic strain, in an in vitro model of B. cereus infection. The presence of the probiotic strain modulates cell response both in infected epithelial and dendritic cells thus suggesting a possible beneficial effect of selected lactobacilli strains on the course of B. cereus infection.
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-
Ashraf, R. and Shah, N.P., 2014. Immune system stimulation by probiotic microorganisms. Critical Reviews in Food Science and Nutrition 54: 938-956.
'Immune system stimulation by probiotic microorganisms ' () 54 Critical Reviews in Food Science and Nutrition : 938 -956.
-
Baldwin, A.S.J., 1996. The NF-κB and IκB proteins: new discoveries and insights. Annual Review of Immunology 14: 649-681.
'The NF-κB and IκB proteins: new discoveries and insights ' () 14 Annual Review of Immunology : 649 -681.
-
Bottone, E.J., 2010. Bacillus cereus, a volatile human pathogen. Clinical Microbiology Reviews 23: 382-398.
'Bacillus cereus, a volatile human pathogen ' () 23 Clinical Microbiology Reviews : 382 -398.
-
Buchanan, R.L. and Schultz, F.J., 1992. Evaluation of the oxoid BCET-RPLA kit for the detection of Bacillus cereus diarrheal enterotoxin as compared to cell culture cytotonicity. Journal of Food Protection 55: 440-443.
'Evaluation of the oxoid BCET-RPLA kit for the detection of Bacillus cereus diarrheal enterotoxin as compared to cell culture cytotonicity ' () 55 Journal of Food Protection : 440 -443.
-
Canny, G., Swidsinski, A. and McCormick, B.A., 2006. Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences. Methods in Molecular Biology 341: 17-35.
'Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences ' () 341 Methods in Molecular Biology : 17 -35.
-
Carasi, P., Racedo, S.M., Jacquot, C., Romanin, D.E., Serradell, M.A. and Urdaci, M.C., 2015. Impact of kefir derived Lactobacillus kefiri on the mucosal immune response and gut microbiota. Journal of Immunological Research 2015: 361604.
'Impact of kefir derived Lactobacillus kefiri on the mucosal immune response and gut microbiota ' () 2015 Journal of Immunological Research : 361604.
-
Cerf-Bensussan, N. and Gaboriau-Routhiau, V., 2010. The immune system and the gut microbiota: friends or foes? Nature Reviews Immunology 10: 735-744.
'The immune system and the gut microbiota: friends or foes? ' () 10 Nature Reviews Immunology : 735 -744.
-
Chen, K., 2013. Lactobacillus acidophilus strain suppresses the transcription of proinflammatory-related factors in human HT-29 cells. Journal of Microbiology and Biotechnology 23: 64-68.
'Lactobacillus acidophilus strain suppresses the transcription of proinflammatory-related factors in human HT-29 cells ' () 23 Journal of Microbiology and Biotechnology : 64 -68.
-
Ditu, L.M., Chifiriuc, M.C., Bezirtzoglou, E., Marutescu, L., Bleotu, C., Pelinescu, D., Mihaescu, G. and Lazar, V., 2014. Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus on holoxenic mice. Microbial Ecology in Health and Disease 2014: 25.
'Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus on holoxenic mice ' () 2014 Microbial Ecology in Health and Disease : 25.
-
Dogi, C.A., Weill, F. and Perdigón, G., 2010. Immune response of non-pathogenic Gram(+) and Gram(−) bacteria in inductive sites of the intestinal mucosa: study of the pathway of signaling involved. Immunobiology 215: 60-69.
'Immune response of non-pathogenic Gram(+) and Gram(−) bacteria in inductive sites of the intestinal mucosa: study of the pathway of signaling involved ' () 215 Immunobiology : 60 -69.
-
Edwards, L.A., Bajaj-Elliott, M., Klein, N.J., Murch, S.H. and Phillips, A.D., 2011. Bacterial-epithelial contact is a key determinant of host innate immune responses to enteropathogenic and enteroaggregative Escherichia coli. PloS One 6: e27030.
'Bacterial-epithelial contact is a key determinant of host innate immune responses to enteropathogenic and enteroaggregative Escherichia coli ' () 6 PloS One : e27030.
-
Fang, H.W., Fang, S.B., Chiang Chiau, J.S., Yeung, C.Y., Chan, W.T., Jiang, C.B., Cheng, M.L. and Lee, H.C., 2010. Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells. Journal of Medical Microbiology 59: 573-579.
'Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells ' () 59 Journal of Medical Microbiology : 573 -579.
-
Franco, M.C., Golowczyc, M.A., De Antoni, G.L., Perez, P.F., Humen, M. and Serradell Mde, L., 2013. Administration of kefir-fermented milk protects mice against Giardia intestinalis infection. Journal of Medical Microbiology 62: 1815-1822.
'Administration of kefir-fermented milk protects mice against Giardia intestinalis infection ' () 62 Journal of Medical Microbiology : 1815 -1822.
-
Galdeano, C.M. and Perdigón, G., 2006. The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clinical and Vaccine Immunology 13: 219-226.
'The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity ' () 13 Clinical and Vaccine Immunology : 219 -226.
-
Garrote, G.L., Abraham, A.G. and Rumbo, M., 2015. Is lactate an undervalued functional component of fermented food products? Frontiers in Microbiology 6: 629.
'Is lactate an undervalued functional component of fermented food products? ' () 6 Frontiers in Microbiology : 629.
-
Gohar, M., Okstad, O.A., Gilois, N., Sanchis, V., Kolsto, A.B. and Lereclus, D., 2002. Two-dimensional electrophoresis analysis of the extracellular proteome of Bacillus cereus reveals the importance of the PlcR regulon. Proteomics 2: 784-791.
'Two-dimensional electrophoresis analysis of the extracellular proteome of Bacillus cereus reveals the importance of the PlcR regulon ' () 2 Proteomics : 784 -791.
-
Grompone, G., Martorell, P., Llopis, S., Gonzalez, N., Genoves, S., Mulet, A.P., Fernandez-Calero, T., Tiscornia, I., Bollati-Fogolin, M., Chambaud, I., Foligne, B., Montserrat, A. and Ramon, D., 2012. Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans. PloS One 7: e52493.
'Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans ' () 7 PloS One : e52493.
-
Guimarães, F.S.F., Andrade, L.F., Martins, S.T., Abud, A.P.R., Sene, R.V., Wanderer, C., Tiscornia, I., Bollati-Fogolín, M., Buchi, D.F. and Trindade, E.S., 2010. In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model. BMC Cancer 10: 113.
'In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model ' () 10 BMC Cancer : 113.
-
Haller, D., Bode, C., Hammes, W.P., Pfeifer, A.M., Schiffrin, E.J. and Blum, S., 2000. Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut 47: 79-87.
'Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures ' () 47 Gut : 79 -87.
-
Hiramatsu, Y., Satho, T., Irie, K., Shiimura, S., Okuno, T., Sharmin, T., Uyeda, S., Fukumitsu, Y., Nakashima, Y., Miake, F. and Kashige, N., 2013. Differences in TLR9-dependent inhibitory effects of H2O2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species. Microbes and Infection 15: 96-104.
'Differences in TLR9-dependent inhibitory effects of H2O2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species ' () 15 Microbes and Infection : 96 -104.
-
Hua, M.-C., Lin, T.-Y., Lai, M.-W., Kong, M.-S., Chang, H.-J. and Chen, C.-C., 2010. Probiotic bio-three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells. World Journal of Gastroenterology 16: 3529-3540.
'Probiotic bio-three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells ' () 16 World Journal of Gastroenterology : 3529 -3540.
-
Huang, I.F., Lin, I.C., Liu, P.F., Cheng, M.F., Liu, Y.C., Hsieh, Y.D., Chen, J.J., Chen, C.L., Chang, H.W. and Shu, C.W., 2015. Lactobacillus acidophilus attenuates Salmonella-induced intestinal inflammation via TGF-β signaling. BMC Microbiology 15: 203.
'Lactobacillus acidophilus attenuates Salmonella-induced intestinal inflammation via TGF-β signaling ' () 15 BMC Microbiology : 203.
-
Hugo, A.A., De Antoni, G.L. and Pérez, P.F., 2006. Lactobacillus delbrueckii subsp lactis strain CIDCA 133 inhibits nitrate reductase activity of Escherichia coli. International Journal of Food Microbiology 111: 191-196.
'Lactobacillus delbrueckii subsp lactis strain CIDCA 133 inhibits nitrate reductase activity of Escherichia coli ' () 111 International Journal of Food Microbiology : 191 -196.
-
Hugo, A.A., De Antoni, G.L. and Pérez, P.F., 2010. Lactobacillus delbrueckii subsp lactis (strain CIDCA 133) resists the antimicrobial activity triggered by molecules derived from enterocyte-like Caco-2 cells. Letters in Applied Microbiology 50: 335-340.
'Lactobacillus delbrueckii subsp lactis (strain CIDCA 133) resists the antimicrobial activity triggered by molecules derived from enterocyte-like Caco-2 cells ' () 50 Letters in Applied Microbiology : 335 -340.
-
Hugo, A.A., Kakisu, E., De Antoni, G.L. and Pérez, P.F., 2008. Lactobacilli antagonize biological effects of enterohaemorrhagic Escherichia coli in vitro. Letters in Applied Microbiology 46: 613-619.
'Lactobacilli antagonize biological effects of enterohaemorrhagic Escherichia coli in vitro ' () 46 Letters in Applied Microbiology : 613 -619.
-
Imaoka, A., Shima, T., Kato, K., Mizuno, S., Uehara, T., Matsumoto, S., Setoyama, H., Hara, T. and Umesaki, Y., 2008. Anti-inflammatory activity of probiotic Bifidobacterium: enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells. World Journal of Gastroenterology 14: 2511-2516.
'Anti-inflammatory activity of probiotic Bifidobacterium: enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells ' () 14 World Journal of Gastroenterology : 2511 -2516.
-
Iraporda, C., Errea, A., Romanin, D.E., Cayet, D., Pereyra, E., Pignataro, O., Sirard, J.C., Garrote, G.L., Abraham, A.G. and Rumbo, M., 2015. Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells. Immunobiology 220: 1161-1169.
'Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells ' () 220 Immunobiology : 1161 -1169.
-
Jeon, J.H., Lee, H.R., Cho, M.H., Park, O.K., Park, J. and Rhie, G.E. 2015. The poly-γ-d-glutamic acid capsule surrogate of the Bacillus anthracis capsule is a novel Toll-like receptor 2 agonist. Infection and Immunity 83: 3847-3856.
'The poly-γ-d-glutamic acid capsule surrogate of the Bacillus anthracis capsule is a novel Toll-like receptor 2 agonist ' () 83 Infection and Immunity : 3847 -3856.
-
Jung, H.C., Eckmann, L., Yang, S.K., Panja, A., Fierer, J., Morzycka-Wroblewska, E. and Kagnoff, M.F., 1995. A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion. Journal of Clinical Investigation 95: 55-65.
'A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion ' () 95 Journal of Clinical Investigation : 55 -65.
-
Jung, T.H., Park, J.H., Jeon, W.M. and Han, K.S., 2015. Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway. Nutrition Research and Practice 9: 343-349.
'Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway ' () 9 Nutrition Research and Practice : 343 -349.
-
Kaji, R., Kiyoshima-Shibata, J., Nagaoka, M., Nanno, M. and Shida, K., 2010. Bacterial teichoic acids reverse predominant IL-12 production induced by certain lactobacillus strains into predominant IL-10 production via TLR2-dependent ERK activation in macrophages. Journal of Immunology 184: 3505-3513.
'Bacterial teichoic acids reverse predominant IL-12 production induced by certain lactobacillus strains into predominant IL-10 production via TLR2-dependent ERK activation in macrophages ' () 184 Journal of Immunology : 3505 -3513.
-
Kang, H. and Im, S., 2015. Probiotics as an immune modulator. Journal of Nutritional Science and Vitaminology 61: 103-105.
'Probiotics as an immune modulator ' () 61 Journal of Nutritional Science and Vitaminology : 103 -105.
-
Karlsson, M., Scherbak, N., Reid, G. and Jass, J., 2012. Lactobacillus rhamnosus GR-1 enhances NF-kappaB activation in Escherichia coli-stimulated urinary bladder cells through TLR4. BMC Microbiology 12: 15.
'Lactobacillus rhamnosus GR-1 enhances NF-kappaB activation in Escherichia coli-stimulated urinary bladder cells through TLR4 ' () 12 BMC Microbiology : 15.
-
Kechaou, N., Chain, F., Gratadoux, J.J., Blugeon, S., Bertho, N., Chevalier, C., Le Goffic, R., Courau, S., Molimard, P., Chatel, J.M., Langella, P. and Bermudez-Humaran, L.G., 2013. Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening. Applied and Environmental Microbiology 79: 1491-1499.
'Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening ' () 79 Applied and Environmental Microbiology : 1491 -1499.
-
Klingspor, S., Bondzio, A., Martens, H., Aschenbach, J.R., Bratz, K., Tedin, K., Einspanier, R. and Lodemann, U., 2015. Enterococcus faecium NCIMB 10415 modulates epithelial integrity, heat shock protein, and proinflammatory cytokine response in intestinal cells. Mediators of Inflammation 2015: 304149
'Enterococcus faecium NCIMB 10415 modulates epithelial integrity, heat shock protein, and proinflammatory cytokine response in intestinal cells ' () 2015 Mediators of Inflammation : 304149.
-
Kim, H., Jung, B.J., Jung, J.H., Kim, J.Y., Chung, S.K. and Chung, D.K., 2012a. Lactobacillus plantarum lipoteichoic acid alleviates TNF-alpha-induced inflammation in the HT-29 intestinal epithelial cell line. Molecules and Cells 33: 479-486.
'Lactobacillus plantarum lipoteichoic acid alleviates TNF-alpha-induced inflammation in the HT-29 intestinal epithelial cell line ' () 33 Molecules and Cells : 479 -486.
-
Kim, J.Y., Park, M.S. and Ji, G.E., 2012b. Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells. World Journal of Gastroenterology 18: 1308-1318.
'Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells ' () 18 World Journal of Gastroenterology : 1308 -1318.
-
Kociubinski, G., Perez, P. and De Antoni, G., 1999. Screening of bile resistance and bile precipitation in lactic acid bacteria and bifidobacteria. Journal of Food Protection 62: 905-912.
'Screening of bile resistance and bile precipitation in lactic acid bacteria and bifidobacteria ' () 62 Journal of Food Protection : 905 -912.
-
Kociubinski, G.L., Pérez, P.F., Añón, M.C. and De Antoni, G.L., 1996. A method of screening for highly inhibitory lactic acid bacteria. Journal of Food Protection 59: 739-745.
'A method of screening for highly inhibitory lactic acid bacteria ' () 59 Journal of Food Protection : 739 -745.
-
Lammers, K.M., Helwig, U., Swennen, E., Rizzello, F., Venturi, A., Caramelli, E., Kamm, M.A., Brigidi, P., Gionchetti, P. and Campieri, M., 2002. Effect of probiotic strains on interleukin 8 production by HT29/19A cells. American Journal of Gastroenterology 97: 1182-1186.
'Effect of probiotic strains on interleukin 8 production by HT29/19A cells ' () 97 American Journal of Gastroenterology : 1182 -1186.
-
Linares, D.M., Ross, P. and Stanton, C., 2015. Beneficial microbes: the pharmacy in the gut. Bioengineered 28: 1-28.
'Beneficial microbes: the pharmacy in the gut ' () 28 Bioengineered : 1 -28.
-
Macpherson, A.J. and Uhr, T., 2004. Induction of protective IgA by intestinal dendritic cells carrying commensal bacteria. Science 303: 1662-1665.
'Induction of protective IgA by intestinal dendritic cells carrying commensal bacteria ' () 303 Science : 1662 -1665.
-
Marchesi, J.R., Adams, D.H., Fava, F., Hermes, G.D., Hirschfield, G.M., Hold, G., Quraishi, M.N., Kinross, J., Smidt, H., Tuohy, K.M., Thomas, L.V., Zoetendal, E.G. and Hart, A., 2015. The gut microbiota and host health: a new clinical frontier. Gut 65: 330-339.
'The gut microbiota and host health: a new clinical frontier ' () 65 Gut : 330 -339.
-
Martin, R., Miquel, S., Ulmer, J., Langella, P. and Bermudez-Humaran, L.G., 2014. Gut ecosystem: how microbes help us. Beneficial Microbes 5: 219-233.
'Gut ecosystem: how microbes help us ' () 5 Beneficial Microbes : 219 -233.
-
Mastropietro, G., Tiscornia, I., Perelmuter, K., Astrada, S. and Bollati-Fogolín, M., 2015. HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation. Mediators of Inflammation 2015: 860534.
'HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation ' () 2015 Mediators of Inflammation : 860534.
-
Matsuguchi, T., Takagi, A., Matsuzaki, T., Nagaoka, M., Ishikawa, K., Yokokura, T. and Yoshikai, Y., 2003. Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2. Clinical and Diagnostic Laboratory Immunology 10: 259-266.
'Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2 ' () 10 Clinical and Diagnostic Laboratory Immunology : 259 -266.
-
Mileti, E., Matteoli, G., Iliev, I.D. and Rescigno, M., 2009. Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy. PloS One 4: e7056.
'Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy ' () 4 PloS One : e7056.
-
Minnaard, J., Delfederico, L., Vasseur, V., Hollmann, A., Rolny, I., Semorile, L. and Perez, P.F., 2007. Virulence of Bacillus cereus: a multivariate analysis. International Journal of Food Microbiology 116: 197-206.
'Virulence of Bacillus cereus: a multivariate analysis ' () 116 International Journal of Food Microbiology : 197 -206.
-
Minnaard, J., Humen, M. and Perez, P.F., 2001. Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells. Journal of Food Protection 64: 1535-1541.
'Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells ' () 64 Journal of Food Protection : 1535 -1541.
-
Minnaard, J., Lievin-Le Moal, V., Coconnier, M.H., Servin, A.L. and Perez, P.F., 2004. Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells. Infection and Immunity 72: 3106-3112.
'Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells ' () 72 Infection and Immunity : 3106 -3112.
-
Minnaard, J., Rolny, I.S. and Perez, P.F., 2013. Interaction between Bacillus cereus and cultured human enterocytes: effect of calcium, cell differentiation, and bacterial extracellular factors. Journal of Food Protection 76: 820-826.
'Interaction between Bacillus cereus and cultured human enterocytes: effect of calcium, cell differentiation, and bacterial extracellular factors ' () 76 Journal of Food Protection : 820 -826.
-
Mizoguchi, E., Hachiya, Y., Kawada, M., Nagatani, K., Ogawa, A., Sugimoto, K., Mizoguchi, A. and Podolsky, D.K., 2008. TNF receptor Type I-dependent activation of innate responses to reduce intestinal damage-associated mortality. Gastroenterology 134: 470-480.
'TNF receptor Type I-dependent activation of innate responses to reduce intestinal damage-associated mortality ' () 134 Gastroenterology : 470 -480.
-
Mohamadzadeh, M., Olson, S., Kalina, W.V., Ruthel, G., Demmin, G.L., Warfield, K.L., Bavari, S. and Klaenhammer, T.R., 2005. Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization. Proceedings of the National Academy of Sciences of the USA 102: 2880-2885.
'Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization ' () 102 Proceedings of the National Academy of Sciences of the USA : 2880 -2885.
-
O’Hara, A.M., O’Regan, P., Fanning, A., O’Mahony, C., Macsharry, J., Lyons, A., Bienenstock, J., O’Mahony, L. and Shanahan, F., 2006. Functional modulation of human intestinal epithelial cell responses by Bifidobacterium infantis and Lactobacillus salivarius. Immunology 118: 202-215.
'Functional modulation of human intestinal epithelial cell responses by Bifidobacterium infantis and Lactobacillus salivarius ' () 118 Immunology : 202 -215.
-
Pagnini, C., Saeed, R., Bamias, G., Arseneau, K.O., Pizarro, T.T. and Cominelli, F., 2010. Probiotics promote gut health through stimulation of epithelial innate immunity. Proceedings of the National Academy of Sciences of the USA 107: 454-459.
'Probiotics promote gut health through stimulation of epithelial innate immunity ' () 107 Proceedings of the National Academy of Sciences of the USA : 454 -459.
-
Papadakos, K.S., Sougleri, I.S., Mentis, A.F., Hatziloukas, E. and Sgouras, D.N., 2013. Presence of terminal EPIYA phosphorylation motifs in Helicobacter pylori CagA contributes to IL-8 secretion, irrespective of the number of repeats. PloS One 8: e56291.
'Presence of terminal EPIYA phosphorylation motifs in Helicobacter pylori CagA contributes to IL-8 secretion, irrespective of the number of repeats ' () 8 PloS One : e56291.
-
Parvez, S., Malik, K.A., Ah Kang, S. and Kim, H.Y., 2006. Probiotics and their fermented food products are beneficial for health. Journal of Applied Microbiology 100: 1171-1185.
'Probiotics and their fermented food products are beneficial for health ' () 100 Journal of Applied Microbiology : 1171 -1185.
-
Pozo-Rubio, T., Mujico, J.R., Marcos, A., Puertollano, E., Nadal, I., Sanz, Y. and Nova, E., 2011. Immunostimulatory effect of faecal Bifidobacterium species of breast-fed and formula-fed infants in a peripheral blood mononuclear cell/Caco-2 co-culture system. British Journal of Nutrition 106: 1216-1223.
'Immunostimulatory effect of faecal Bifidobacterium species of breast-fed and formula-fed infants in a peripheral blood mononuclear cell/Caco-2 co-culture system ' () 106 British Journal of Nutrition : 1216 -1223.
-
Ramadan, R.T., Moyer, A.L. and Callegan, M.C., 2008. A role for tumor necrosis factor-alpha in experimental Bacillus cereus endophthalmitis pathogenesis. Investigative Ophthalmology and Visual Science 49: 4482-4489.
'A role for tumor necrosis factor-alpha in experimental Bacillus cereus endophthalmitis pathogenesis ' () 49 Investigative Ophthalmology and Visual Science : 4482 -4489.
-
Rescigno, M., 2010. Intestinal dendritic cells. Advances in Immunology 107: 109-138.
'Intestinal dendritic cells ' () 107 Advances in Immunology : 109 -138.
-
Riedel, C.U., Foata, F., Goldstein, D.R., Blum, S. and Eikmanns, B.J., 2006. Interaction of bifidobacteria with Caco-2 cells – adhesion and impact on expression profiles. International Journal of Food Microbiology 110: 62-68.
'Interaction of bifidobacteria with Caco-2 cells – adhesion and impact on expression profiles ' () 110 International Journal of Food Microbiology : 62 -68.
-
Roberts-Thomson, I.C., Fon, J., Uylaki, W., Cummins, A.G. and Barry, S., 2011. Cells, cytokines and inflammatory bowel disease: a clinical perspective. Expert Reviews on Gastroenterology and Hepatology 5: 703-716.
'Cells, cytokines and inflammatory bowel disease: a clinical perspective ' () 5 Expert Reviews on Gastroenterology and Hepatology : 703 -716.
-
Rolny, I.S., Minnaard, J., Racedo, S.M. and Perez, P.F., 2014. Murine model of Bacillus cereus gastrointestinal infection. Journal of Medical Microbiology 63: 1741-1749.
'Murine model of Bacillus cereus gastrointestinal infection ' () 63 Journal of Medical Microbiology : 1741 -1749.
-
Romanin, D., Serradell, M., Gonzalez Maciel, D., Lausada, N., Garrote, G.L. and Rumbo, M., 2010. Down-regulation of intestinal epithelial innate response by probiotic yeasts isolated from kefir. International Journal of Food Microbiology 140: 102-108.
'Down-regulation of intestinal epithelial innate response by probiotic yeasts isolated from kefir ' () 140 International Journal of Food Microbiology : 102 -108.
-
Rowan, N.J., Deans, K., Anderson, J.G., Gemmell, C.G., Hunter, I.S. and Chaithong, T., 2001. Putative virulence factor expression by clinical and food isolates of Bacillus spp. after growth in reconstituted infant milk formulae. Applied and Environmental Microbiology 67: 3873-3881.
'Putative virulence factor expression by clinical and food isolates of Bacillus spp. after growth in reconstituted infant milk formulae ' () 67 Applied and Environmental Microbiology : 3873 -3881.
-
Scaldaferri, F., Pizzoferrato, M., Gerardi, V., Lopetuso, L. and Gasbarrini, A., 2012. The gut barrier: new acquisitions and therapeutic approaches. Journal of Clinical Gastroenterology 46: 12-17.
'The gut barrier: new acquisitions and therapeutic approaches ' () 46 Journal of Clinical Gastroenterology : 12 -17.
-
Schulte, R., Wattiau, P., Hartland, E.L., Robins-Browne, R.M. and Cornelis, G.R., 1996. Differential secretion of interleukin-8 by human epithelial cell lines upon entry of virulent or nonvirulent Yersinia enterocolitica. Infection and Immunity 64: 2106-2113.
'Differential secretion of interleukin-8 by human epithelial cell lines upon entry of virulent or nonvirulent Yersinia enterocolitica ' () 64 Infection and Immunity : 2106 -2113.
-
Sokol, H., Pigneur, B., Watterlot, L., Lakhdari, O., Bermúdez-Humarán, L.G., Gratadoux, J.-J., Blugeon, S., Bridonneau, C., Furet, J.-P., Corthier, G., Grangette, C., Vasquez, N., Pochart, P., Trugnan, G., Thomas, G., Blottière, H.M., Doré, J., Marteau, P., Seksik, P. and Langella, P., 2008. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proceedings of the National Academy of Sciences of the USA 105: 16731-16736.
'Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients ' () 105 Proceedings of the National Academy of Sciences of the USA : 16731 -16736.
-
Tanaka, K., Fujiya, M., Konishi, H., Ueno, N., Kashima, S., Sasajima, J., Moriichi, K., Ikuta, K., Tanabe, H. and Kohgo, Y., 2015. Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway. Biochemical and Biophysical Research Communications 467: 541-548.
'Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway ' () 467 Biochemical and Biophysical Research Communications : 541 -548.
-
Tergaonkar, V., 2006. NFκB pathway: a good signaling paradigm and therapeutic target. International Journal of Biochemistry and Cell Biology 38: 1647-1653.
'NFκB pathway: a good signaling paradigm and therapeutic target ' () 38 International Journal of Biochemistry and Cell Biology : 1647 -1653.
-
Tiscornia, I., Sanchez-Martins, V., Hernandez, A. and Bollati-Fogolin, M., 2012. Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells. Journal of Immunological Methods 384: 164-170.
'Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells ' () 384 Journal of Immunological Methods : 164 -170.
-
Tojo, R., Suarez, A., Clemente, M.G., De los Reyes-Gavilan, C.G., Margolles, A., Gueimonde, M. and Ruas-Madiedo, P., 2014. Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis. World Journal of Gastroenterology 20: 15163-15176.
'Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis ' () 20 World Journal of Gastroenterology : 15163 -15176.
-
Torres-Maravilla, E., Lenoir, M., Mayorga-Reyes, L., Allain, T., Sokol, H., Langella, P., Sanchez-Pardo, M.E. and Bermudez-Humaran, L.G., 2015. Identification of novel anti-inflammatory probiotic strains isolated from pulque. Applied Microbiology and Biotechnology.
'Identification of novel anti-inflammatory probiotic strains isolated from pulque ', in Applied Microbiology and Biotechnology , ().
-
Van Baarlen, P., Troost, F.J., Van Hemert, S., Van der Meer, C., De Vos, W.M., De Groot, P.J., Hooiveld, G.J., Brummer, R.J. and Kleerebezem, M., 2009. Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance. Proceedings of the National Academy of Sciences of the USA 106: 2371-2376.
'Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance ' () 106 Proceedings of the National Academy of Sciences of the USA : 2371 -2376.
-
Viggiano, D., Ianiro, G., Vanella, G., Bibbò, S., Bruno, G., Simeone, G. and Mele, G., 2015. Gut barrier in health and disease: focus on childhood. European Review for Medical and Pharmacological Sciences 19: 1077-1085.
'Gut barrier in health and disease: focus on childhood ' () 19 European Review for Medical and Pharmacological Sciences : 1077 -1085.
-
Zeuthen, L.H., Christensen, H.R. and Frøkiær, H., 2006. Lactic acid bacteria inducing a weak Interleukin-12 and tumor necrosis factor alpha response in human dendritic cells inhibit strongly stimulating lactic acid bacteria but act synergistically with gram-negative bacteria. Clinical and Vaccine Immunology 13: 365-375.
'Lactic acid bacteria inducing a weak Interleukin-12 and tumor necrosis factor alpha response in human dendritic cells inhibit strongly stimulating lactic acid bacteria but act synergistically with gram-negative bacteria ' () 13 Clinical and Vaccine Immunology : 365 -375.
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Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus.
In: Beneficial MicrobesSearch for other papers by I.S. Rolny in
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Laboratorio de Biotecnología, Facultad de Ingeniería-Universidad ORT Uruguay, Cuareim 1451, 11100 Montevideo, Uruguay.
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Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Calle 47 y 116, B1900AJI La Plata, Argentina.
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It is known that probiotic microorganisms are able to modulate pathogen virulence. This ability is strain dependent and involves multiple interactions between microorganisms and relevant host’s cell populations. In the present work we focus on the effect of a potentially probiotic lactobacillus strain (Lactobacillus delbrueckii subsp. lactis CIDCA 133) in an in vitro model of Bacillus cereus infection. Our results showed that infection of intestinal epithelial HT-29 cells by B. cereus induces nuclear factor kappa B (NF-κB) pathway. Noteworthy, the presence of strain L. delbrueckii subsp.lactis CIDCA 133 increases stimulation. However, B. cereus-induced interleukin (IL)-8 production by epithelial cells is partially abrogated by L. delbrueckii subsp. lactis CIDCA 133. These findings suggest that signalling pathways other than that of NF-κB are involved. In a co-culture system (HT-29 and monocyte-derived dendritic cells), B. cereus was able to translocate from the epithelial (upper) to the dendritic cell compartment (lower). This translocation was partially abrogated by the presence of lactobacilli in the upper compartment. In addition, infection of epithelial cells in the co-culture model, led to an increase in the expression of CD86 by dendritic cells. This effect could not be modified in the presence of lactobacilli. Interestingly, infection of enterocytes with B. cereus triggers production of proinflammatory cytokines by dendritic cells (IL-8, IL-6 and tumour necrosis factor alpha (TNF-α)). The production of TNF-α (a protective cytokine in B. cereus infections) by dendritic cells was increased in the presence of lactobacilli. The present work demonstrates for the first time the effect of L. delbrueckii subsp. lactis CIDCA 133, a potentially probiotic strain, in an in vitro model of B. cereus infection. The presence of the probiotic strain modulates cell response both in infected epithelial and dendritic cells thus suggesting a possible beneficial effect of selected lactobacilli strains on the course of B. cereus infection.
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