Bacillus coagulans GBI-30, 6086 increases plant protein digestion in a dynamic, computer-controlled in vitro model of the small intestine (TIM-1)
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The aim of this study was to assess the potential of the probiotic Bacillus coagulans GBI-30, 6086 [GanedenBC30] (BC30) to aid in protein digestion of alimentary plant proteins. To test this, three plant proteins, from pea, soy and rice, were digested in a validated in vitro model of the stomach and small intestine (TIM-1) in the absence and in the presence of BC30. Samples were taken from the TIM-1 fractions that mimic uptake of amino acids by the host and analysed for α-amino nitrogen (AAN) and total nitrogen (TN). Both were increased by BC30 for all three plant proteins sources. The ratio of TN/AAN indicated that for pea protein digestion was increased by BC30, but the degree of polymerisation of the liberated small peptides and free amino acids was not changed. For soy and rice, however, BC30 showed a 2-fold reduction in the TN/AAN ratio, indicating that the liberated digestion products formed during digestion in the presence of BC30 were shorter peptides and more free amino acids, than those liberated in the absence of BC30. As BC30 increased protein digestion and uptake in the upper gastrointestinal (GI) tract, it consequently also reduced the amount of protein that would be delivered to the colon, which could there be fermented into toxic metabolites by the gut microbiota. Thus, the enhanced protein digestion by BC30 showed a dual benefit: enhanced amino acid bioavailability from plant proteins in the upper GI tract, and a healthier environment in the colon.
-
Andriamihaja, M., Guillot, A., Svendsen, A., Hagedorn, J., Rakotondratohanina, S., Tome, D. and Blachier, F., 2013. Comparative efficiency of microbial enzyme preparations versus pancreatin for in vitro alimentary protein digestion. Amino Acids 44: 563-572.
'Comparative efficiency of microbial enzyme preparations versus pancreatin for in vitro alimentary protein digestion ' () 44 Amino Acids : 563 -572.
-
Anonymous, 2004. The first quantitative evidence proving the efficacy of supplemental enzymes. National enzyme company. Available at: http://tinyurl.com/zkff65x.
-
Baron, M., 2009. A patented strain of Bacillus coagulans increased immune response to viral challenge. Postgraduate Medicine 121: 114-118.
'A patented strain of Bacillus coagulans increased immune response to viral challenge ' () 121 Postgraduate Medicine : 114 -118.
-
Cowieson, A.J., Acamovic, T. and Bedford, M.R., 2003. Supplementation of diets containing pea meal with exogenous enzymes: effects on weight gain, feed conversion, nutrient digestibility and gross morphology of the gastrointestinal tract of growing broiler chicks. British Poultry Science 44: 427-437.
'Supplementation of diets containing pea meal with exogenous enzymes: effects on weight gain, feed conversion, nutrient digestibility and gross morphology of the gastrointestinal tract of growing broiler chicks ' () 44 British Poultry Science : 427 -437.
-
Dolin, B.J., 2009. Effects of a proprietary Bacillus coagulans preparation on symptoms of diarrhea-predominant irritable bowel syndrome. Methods and Findings in Experimental and Clinical Pharmacology 31: 655-659.
'Effects of a proprietary Bacillus coagulans preparation on symptoms of diarrhea-predominant irritable bowel syndrome ' () 31 Methods and Findings in Experimental and Clinical Pharmacology : 655 -659.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Available at: http://tinyurl.com/8bccc3r.
-
Hatanaka, M., Nakamura, Y., Maathuis, A.J., Venema, K., Murota, I. and Yamamoto, N., 2012. Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions. Beneficial Microbes 3: 229-236.
'Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions ' () 3 Beneficial Microbes : 229 -236.
-
Havenaar, R., Anneveld, B., Hanff, L.M., De Wildt, S.N., De Koning, B.A., Mooij, M.G., Lelieveld, J.P. and Minekus, M., 2013. In vitro gastrointestinal model (TIM) with predictive power, even for infants and children? International Journal of Pharmaceutics 457: 327-332.
'In vitro gastrointestinal model (TIM) with predictive power, even for infants and children? ' () 457 International Journal of Pharmaceutics : 327 -332.
-
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L., Canani, R.B., Flint, H.J., Salminen, S., Calder, P.C. and Sanders, M.E., 2014. Expert consensus document. The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology and Hepatology 11: 506-514.
'Expert consensus document. The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic ' () 11 Nature Reviews Gastroenterology and Hepatology : 506 -514.
-
Hun, L., 2009. Bacillus coagulans significantly improved abdominal pain and bloating in patients with IBS. Postgraduate Medicine 121: 119-124.
'Bacillus coagulans significantly improved abdominal pain and bloating in patients with IBS ' () 121 Postgraduate Medicine : 119 -124.
-
Jager, R., Shields, K.A., Lowery, R.P., De Souza, E.O., Partl, J.M., Hollmer, C., Purpura, M. and Wilson, J.M., 2016. Probiotic Bacillus coagulans GBI-30, 6086 reduces exercise-induced muscle damage and increases recovery. PeerJ 4: e2276.
'Probiotic Bacillus coagulans GBI-30, 6086 reduces exercise-induced muscle damage and increases recovery ' () 4 PeerJ : e2276.
-
Kalman, D.S., Schwartz, H.I., Alvarez, P., Feldman, S., Pezzullo, J.C. and Krieger, D.R., 2009. A prospective, randomized, double-blind, placebo-controlled parallel-group dual site trial to evaluate the effects of a Bacillus coagulans-based product on functional intestinal gas symptoms. BMC Gastroenterology 9: 85.
'A prospective, randomized, double-blind, placebo-controlled parallel-group dual site trial to evaluate the effects of a Bacillus coagulans-based product on functional intestinal gas symptoms ' () 9 BMC Gastroenterology : 85.
-
Kimmel, M., Keller, D., Farmer, S. and Warrino, D.E., 2010. A controlled clinical trial to evaluate the effect of GanedenBC(30) on immunological markers. Methods and Findings in Experimental and Clinical Pharmacology 32: 129-132.
'A controlled clinical trial to evaluate the effect of GanedenBC(30) on immunological markers ' () 32 Methods and Findings in Experimental and Clinical Pharmacology : 129 -132.
-
Lankisch, P.G., Lembcke, B., Wemken, G. and Creutzfeldt, W., 1986. Functional reserve capacity of the exocrine pancreas. Digestion 35: 175-181.
'Functional reserve capacity of the exocrine pancreas ' () 35 Digestion : 175 -181.
-
Layer, P., Keller, J. and Lankisch, P.G., 2001. Pancreatic enzyme replacement therapy. Current Gastroenterology Reports 3: 101-108.
'Pancreatic enzyme replacement therapy ' () 3 Current Gastroenterology Reports : 101 -108.
-
Maathuis, A.J., Keller, D. and Farmer, S., 2010. Survival and metabolic activity of the GanedenBC30 strain of Bacillus coagulans in a dynamic in vitro model of the stomach and small intestine. Beneficial Microbes 1: 31-36.
'Survival and metabolic activity of the GanedenBC30 strain of Bacillus coagulans in a dynamic in vitro model of the stomach and small intestine ' () 1 Beneficial Microbes : 31 -36.
-
Mandel, D.R., Eichas, K. and Holmes, J., 2010. Bacillus coagulans: a viable adjunct therapy for relieving symptoms of rheumatoid arthritis according to a randomized, controlled trial. BMC Complementary and Alternative Medicine 10: 1.
'Bacillus coagulans: a viable adjunct therapy for relieving symptoms of rheumatoid arthritis according to a randomized, controlled trial ' () 10 BMC Complementary and Alternative Medicine : 1.
-
Marteau, P., Minekus, M., Havenaar, R. and Huis in’t Veld, J.H., 1997. Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile. Journal of Dairy Science 80: 1031-1037.
'Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile ' () 80 Journal of Dairy Science : 1031 -1037.
-
Minekus, M., 1998. Development and validation of a dynamic model of the gastrointestinal tract. Ph.D. thesis, Utrecht University, Utrecht, the Netherlands.
-
Minekus, M., Marteau, P., Havenaar, R. and Huis in’t Veld, J.H.J., 1995. A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine. Alternatives to Laboratory Animals 23: 197-209.
'A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine ' () 23 Alternatives to Laboratory Animals : 197 -209.
-
Minevich, J., Olson, M.A., Mannion, J.P., Boublik, J.H., McPherson, J.O., Lowery, R.P., Shields, K., Sharp, M., De Souza, E.O., Wilson, J.M., Purpura, M. and Jäger, R., 2015. Digestive enzymes reduce quality differences between plant and animal proteins: a double-blind crossover study. Journal of the International Society of Sports Nutrition 12: P26-P26.
'Digestive enzymes reduce quality differences between plant and animal proteins: a double-blind crossover study ' () 12 Journal of the International Society of Sports Nutrition : P26 -P26.
-
Orru, L., Salvetti, E., Cattivelli, L., Lamontanara, A., Michelotti, V., Capozzi, V., Spanoc, Keller, K., Cash, H., Martina, A., Torriani, S. and Felis, G.E., 2014. Draft genome sequence of Bacillus coagulans GBI-30, 6086, a widely used spore-forming probiotic strain. Genome Announcements 2(6): e01080-14.
'Draft genome sequence of Bacillus coagulans GBI-30, 6086, a widely used spore-forming probiotic strain ' () 2 Genome Announcements : e01080 -14.
-
Sikkens, E.C., Cahen, D.L., Kuipers, E.J. and Bruno, M.J., 2010. Pancreatic enzyme replacement therapy in chronic pancreatitis. Best Practice and Research: Clinical Gastroenterology 24: 337-347.
'Pancreatic enzyme replacement therapy in chronic pancreatitis ' () 24 Best Practice and Research: Clinical Gastroenterology : 337 -347.
-
Smeets-Peeters, M.J., Minekus, M., Havenaar, R., Schaafsma, G. and Verstegen, M.W., 1999. Description of a dynamic in vitro model of the dog gastrointestinal tract and an evaluation of various transit times for protein and calcium. Alternatives to Laboratory Animals 27: 935-949.
'Description of a dynamic in vitro model of the dog gastrointestinal tract and an evaluation of various transit times for protein and calcium ' () 27 Alternatives to Laboratory Animals : 935 -949.
-
Van Nuenen, M.H.M.C., Meyer, P.D. and Venema, K., 2003. The effect of various inulins and Clostridium difficile on the metabolic activity of the human colonic microbiota in vitro. Microbial Ecology in Health and Disease 15: 137-144.
'The effect of various inulins and Clostridium difficile on the metabolic activity of the human colonic microbiota in vitro ' () 15 Microbial Ecology in Health and Disease : 137 -144.
-
Venema, K., 2010. Role of gut microbiota in the control of energy and carbohydrate metabolism. Current Opinion in Clinical Nutrition and Metabolic Care 13: 432-438.
'Role of gut microbiota in the control of energy and carbohydrate metabolism ' () 13 Current Opinion in Clinical Nutrition and Metabolic Care : 432 -438.
-
Venema, K., Havenaar, R. and Minekus, M., 2009. Improving in vitro simulation of the GI-tract – models that mimic the stomach and intestines. In: McClements, J.D. and Decker, E.A. (eds.) Designing functional foods – Measuring and controlling food structure breakdown and nutrient absorption. Woodhead Publishing Ltd., Cambridge, UK, pp. 314-229.
'Improving in vitro simulation of the GI-tract – models that mimic the stomach and intestines ', in Designing functional foods – Measuring and controlling food structure breakdown and nutrient absorption , () 314 -229.
Supplementary Materials
Bacillus coagulans GBI-30, 6086 increases plant protein digestion in a dynamic, computer-controlled in vitro model of the small intestine (TIM-1)
In: Beneficial MicrobesSearch for other papers by D. Keller in
Current site
Google Scholar
PubMed
Search for other papers by R. Van Dinter in
Current site
Google Scholar
PubMed
Search for other papers by H. Cash in
Current site
Google Scholar
PubMed
Search for other papers by S. Farmer in
Current site
Google Scholar
PubMed
Beneficial Microbes Consultancy, Johan Karschstraat 3, 6709 TN Wageningen, the Netherlands.
Search for other papers by K. Venema in
Current site
Google Scholar
PubMed
The aim of this study was to assess the potential of the probiotic Bacillus coagulans GBI-30, 6086 [GanedenBC30] (BC30) to aid in protein digestion of alimentary plant proteins. To test this, three plant proteins, from pea, soy and rice, were digested in a validated in vitro model of the stomach and small intestine (TIM-1) in the absence and in the presence of BC30. Samples were taken from the TIM-1 fractions that mimic uptake of amino acids by the host and analysed for α-amino nitrogen (AAN) and total nitrogen (TN). Both were increased by BC30 for all three plant proteins sources. The ratio of TN/AAN indicated that for pea protein digestion was increased by BC30, but the degree of polymerisation of the liberated small peptides and free amino acids was not changed. For soy and rice, however, BC30 showed a 2-fold reduction in the TN/AAN ratio, indicating that the liberated digestion products formed during digestion in the presence of BC30 were shorter peptides and more free amino acids, than those liberated in the absence of BC30. As BC30 increased protein digestion and uptake in the upper gastrointestinal (GI) tract, it consequently also reduced the amount of protein that would be delivered to the colon, which could there be fermented into toxic metabolites by the gut microbiota. Thus, the enhanced protein digestion by BC30 showed a dual benefit: enhanced amino acid bioavailability from plant proteins in the upper GI tract, and a healthier environment in the colon.