Regulatory T cells and Toll-like receptor 2 and 4 mRNA expression in infants with colic treated with Lactobacillus reuteri DSM17938
In: Beneficial MicrobesSearch for other papers by F. Savino in
Current site
Google Scholar
Search for other papers by I. Galliano in
Current site
Google Scholar
Search for other papers by M. Garro in
Current site
Google Scholar
Search for other papers by A. Savino in
Current site
Google Scholar
Search for other papers by V. Daprà in
Current site
Google Scholar
Search for other papers by P. Montanari in
Current site
Google Scholar
Search for other papers by M. Bergallo in
Current site
Google Scholar
Regulatory T cells induce immune homeostasis and the expression of Toll like receptors (TLRs); subsequent inflammatory cytokine release may be involved. Recent studies have shown a microbial imbalance in the gut of colicky infants (with a prevalence of gram-negative bacteria, such as Escherichia coli), and accumulating evidence has shown the efficacy of a probiotic (Lactobacillus reuteri) in breastfed subjects, but the underlying mechanism remains undefined. The study enrolled 59 infants younger than 60 days, of whom 34 subjects had colic and 25 were healthy controls. With a double-blind, placebo-controlled randomised study performed in our unit from October 2016 to July 2017, infants with colic were randomly assigned to receive oral daily L. reuteri DSM17938 (1×108 cfu) or placebo for 28 days. Peripheral blood was collected to assess the expression of FoxP3, TLR2 and TLR4 mRNA using real-time TaqMan RT-PCR at baseline and after the study period. Our findings showed increased mRNA expression of the transcription factor forkhead box P3 (FoxP3) in infants treated with L. reuteri DSM 17938 for 28 days (P<0.009) and increased TLR2 and TLR4 mRNA expression in both treated and placebo subjects. After L. reuteri administration for 28 days in infants with colic, we observed a significant decrease in daily crying time (302.3±19.86 min/day on day 0 vs 76.75±22.15 min/day on day 28, P=0.001). This study provides evidence that the observed increase in FoxP3 expression and reduction in crying time might be responses to probiotic treatment, while the increase in TLR2 and TLR4 mRNA expression might be related to age. Exploiting these new findings may lead to an unprecedented level of therapeutic control over immune tolerance using probiotics.
-
Benninga, M.A., Faure, C., Hyman, P.E., St James Roberts, I., Schechter, N.L. and Nurko, S., 2016. Childhood functional gastrointestinal disorders: neonate/toddler. Gastroenterology 150: 1443-1455.
'Childhood functional gastrointestinal disorders: neonate/toddler ' () 150 Gastroenterology : 1443 -1455.
-
Camilleri, M., Park, S.Y., Scarpato, E. and Staiano, A., 2017. Exploring hypotheses and rationale for causes of infantile colic. Neurogastroenterology and Motility 29: e12943.
'Exploring hypotheses and rationale for causes of infantile colic ' () 29 Neurogastroenterology and Motility : e12943.
-
Cervantes-Barragan, L., Chai, J.N., Tianero, M.D., Di Luccia, B., Ahern, P.P., Merriman, J., Cortez, V.S., Caparon, M.G., Donia, M.S., Gilfillan, S., Cella, M., Gordon, J.I., Hsieh, C.S. and Colonna, M., 2017. Lactobacillus reuteri induces gut intraepithelial CD4(+) CD8αα(+) T cells. Science 357: 806-810.
'Lactobacillus reuteri induces gut intraepithelial CD4(+) CD8αα(+) T cells ' () 357 Science : 806 -810.
-
Fan, Y. and Liu, B., 2015. Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis. Experimental and Therapeutic Medicine 9: 1455-1459.
'Expression of Toll-like receptors in the mucosa of patients with ulcerative colitis ' () 9 Experimental and Therapeutic Medicine : 1455 -1459.
-
Flacher, V., Bouschbacher, M., Verronèse, E., Massacrier, C., Sisirak, V., Berthier-Vergnes, O., De Saint-Vis, B., Caux, C., Dezutter-Dambuyant, C., Lebecque, S., Valladeau, J., Bouschbacher, M. and Verronèse, E., 2006. Human Langerhans cells express a specific TLR profile and differentially respond to viruses and gram-positive bacteria. Journal of Immunology 177: 7959-7967.
'Human Langerhans cells express a specific TLR profile and differentially respond to viruses and gram-positive bacteria ' () 177 Journal of Immunology : 7959 -7967.
-
Gensollen, T., Iyer, S.S., Kasper, D.L. and Blumberg, R.S., 2016. How colonization by microbiota in early life shapes the immune system. Science 352: 539-544.
'How colonization by microbiota in early life shapes the immune system ' () 352 Science : 539 -544.
-
Haileselassie, Y., Navis, M., Vu, N., Qazi, K.R., Rethi, B. and Sverremark-Ekström, E., 2016. Lactobacillus reuteri and Staphylococcus aureus differentially influence the generation of monocyte-derived dendritic cells and subsequent autologous T cell responses. Immunity, Inflammation and Disease 4: 315-326.
'Lactobacillus reuteri and Staphylococcus aureus differentially influence the generation of monocyte-derived dendritic cells and subsequent autologous T cell responses ' () 4 Immunity, Inflammation and Disease : 315 -326.
-
He, Y., Lawlor, N.T. and Newburg, D.S., 2016. Human milk components modulate Toll-like receptor-mediated inflammation. Advances in Nutrition 7: 102-111.
'Human milk components modulate Toll-like receptor-mediated inflammation ' () 7 Advances in Nutrition : 102 -111.
-
Hoang, T.K., He, B., Wang, T., Tran, D.Q., Rhoads, J.M. and Liu, Y., 2018. Protective effect of Lactobacillus reuteri DSM 17938 against experimental necrotizing enterocolitis is mediated by Toll-like receptor 2. American Journal of Physiology – Gastrointestinal and Liver Physiology 315: G231-G240.
'Protective effect of Lactobacillus reuteri DSM 17938 against experimental necrotizing enterocolitis is mediated by Toll-like receptor 2 ' () 315 American Journal of Physiology – Gastrointestinal and Liver Physiology : G231 -G240.
-
Hug, H., Mohajeri, M.H. and La Fata, G., 2018. Toll-like receptors: regulators of the immune response in the human gut. Nutrients 10: E203.
'Toll-like receptors: regulators of the immune response in the human gut ' () 10 Nutrients : E203.
-
Inoue, R., Yajima, T. and Tsukahara, T., 2017. Expression of TLR2 and TLR4 in murine small intestine during postnatal development. Bioscience, Biotechnology and Biochemistry 81: 350-358.
'Expression of TLR2 and TLR4 in murine small intestine during postnatal development ' () 81 Bioscience, Biotechnology and Biochemistry : 350 -358.
-
Kawai, T. and Akira, S., 2010. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology 11: 373-384.
'The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors ' () 11 Nature Immunology : 373 -384.
-
Kunze, W.A., Mao, Y.K., Wang, B., Huizinga, J.D., Ma, X., Forsythe, P. and Bienenstock, J., 2009. Lactobacillus reuteri enhances excitability of colonic AH neurons by inhibiting calcium-dependent potassium channel opening. Journal of Cellular and Molecular Medicine 13: 2261-2270.
'Lactobacillus reuteri enhances excitability of colonic AH neurons by inhibiting calcium-dependent potassium channel opening ' () 13 Journal of Cellular and Molecular Medicine : 2261 -2270.
-
Liu, Y., Fatheree, N.Y., Mangalat, N. and Rhoads, J.M., 2012. Lactobacillus reuteri strains reduce incidence and severity of experimental necrotizing enterocolitis via modulation of TLR4 and NF-κB signaling in the intestine. American Journal of Physiology – Gastrointestinal and Liver Physiology 302: G608-G617.
'Lactobacillus reuteri strains reduce incidence and severity of experimental necrotizing enterocolitis via modulation of TLR4 and NF-κB signaling in the intestine ' () 302 American Journal of Physiology – Gastrointestinal and Liver Physiology : G608 -G617.
-
Lu, C.Y. and Ni, Y.H., 2015. Gut microbiota and the development of pediatric diseases. Journal of Gastroenterology 50: 720-726.
'Gut microbiota and the development of pediatric diseases ' () 50 Journal of Gastroenterology : 720 -726.
-
Ma, Y.H., Zhang, J., Chen, X., Xie, Y.F., Pang, Y.H. and Liu, X.J., 2016. Increased CD4(+)CD45RA(-)FoxP3(low) cells alter the balance between Treg and Th17 cells in colitis mice. World Journal of Gastroenterology 22: 9356-9367.
'Increased CD4(+)CD45RA(-)FoxP3(low) cells alter the balance between Treg and Th17 cells in colitis mice ' () 22 World Journal of Gastroenterology : 9356 -9367.
-
Mareschi, K., Castiglia, S., Sanavio, F., Rustichelli, D., Muraro, M., Defedele, D., Bergallo, M. and Fagioli, F., 2016. Immunoregulatory effects on T lymphocytes by human mesenchymal stromal cells isolated from bone marrow, amniotic fluid, and placenta. Experimental Hematology 44: 138-150.
'Immunoregulatory effects on T lymphocytes by human mesenchymal stromal cells isolated from bone marrow, amniotic fluid, and placenta ' () 44 Experimental Hematology : 138 -150.
-
Mogensen, T.H., 2009. Pathogen recognition and inflammatory signaling in innate immune defenses. Clinical Microbiology Reviews 22: 240-273.
'Pathogen recognition and inflammatory signaling in innate immune defenses ' () 22 Clinical Microbiology Reviews : 240 -273.
-
Nakanishi, Y., Ikebuchi, R., Chtanova, T., Kusumoto, Y., Okuyama, H., Moriya, T., Honda, T., Kabashima, K., Watanabe, T., Sakai, Y. and Tomura, M., 2018. Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes. Mucosal Immunology 11: 437-448.
'Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes ' () 11 Mucosal Immunology : 437 -448.
-
Pärtty, A., Kalliomäki, M., Salminen, S. and Isolauri, E., 2017. Infantile colic is associated with low-grade systemic inflammation. Journal of Pediatric Gastroenterology and Nutrition 64: 691-695.
'Infantile colic is associated with low-grade systemic inflammation ' () 64 Journal of Pediatric Gastroenterology and Nutrition : 691 -695.
-
Perez-Burgos, A., Wang, L., McVey Neufeld, K.A., Mao, Y.K., Ahmadzai, M., Janssen, L.J., Stanisz, A.M., Bienenstock, J. and Kunze, W.A., 2015. The TRPV1 channel in rodents is a major target for antinociceptive effect of the probiotic Lactobacillus reuteri DSM 17938. Journal of Physiology 593: 3943-3957.
'The TRPV1 channel in rodents is a major target for antinociceptive effect of the probiotic Lactobacillus reuteri DSM 17938 ' () 593 Journal of Physiology : 3943 -3957.
-
Rachmilewitz, D., Katakura, K., Karmeli, F., Hayashi, T., Reinus, C., Rudensky, B., Akira, S., Takeda, K., Lee, J., Takabayashi, K. and Raz, E., 2004. Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis. Gastroenterology 126: 520-528.
'Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis ' () 126 Gastroenterology : 520 -528.
-
Round, J.L. and Mazmanian, S.K., 2010. Inducible Foxp3+ regulatory T-cell development by a commensal bacterium of the intestinal microbiota. Proceedings of the National Academy of Sciences of the USA 107: 12204-12209.
'Inducible Foxp3+ regulatory T-cell development by a commensal bacterium of the intestinal microbiota ' () 107 Proceedings of the National Academy of Sciences of the USA : 12204 -12209.
-
Savino, F., Cordisco, L., Tarasco, V., Calabrese, R., Palumeri, E. and Matteuzzi, D., 2009. Molecular identification of coliform bacteria from colicky breastfed infants. Acta Paediatrica 98: 1582-1588.
'Molecular identification of coliform bacteria from colicky breastfed infants ' () 98 Acta Paediatrica : 1582 -1588.
-
Savino, F., Cordisco, L., Tarasco, V., Locatelli, E., Di Gioia, D., Oggero, R. and Matteuzzi, D., 2011. Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants. BMC Microbiology 11: 157.
'Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants ' () 11 BMC Microbiology : 157.
-
Savino, F., Cordisco, L., Tarasco, V., Palumeri, E., Calabrese, R., Oggero, R., Roos, S. and Matteuzzi, D., 2010. Lactobacillus reuteri DSM 17938 in infantile colic: a randomized, double-blind, placebo-controlled trial. Pediatrics 126: e526-e533.
'Lactobacillus reuteri DSM 17938 in infantile colic: a randomized, double-blind, placebo-controlled trial ' () 126 Pediatrics : e526 -e533.
-
Savino, F., Galliano, I., Garro, M., Savino, A., Manzoni, P., Daprà, V., Montanari, P. and Bergallo, M., 2018. Changes in the messenger RNA expression of Toll-like receptors 2 and 4 in healthy infants according to age. American Journal of Perinatology 35: 583-588.
'Changes in the messenger RNA expression of Toll-like receptors 2 and 4 in healthy infants according to age ' () 35 American Journal of Perinatology : 583 -588.
-
Sung, V., D’Amico, F., Cabana, M.D., Chau, K., Koren, G., Savino, F., Szajewska, H., Deshpande, G., Dupont, C., Indrio, F., Mentula, S., Partty, A. and Tancredi, D. 2018. Lactobacillus reuteri to treat infant colic: a meta-analysis. Pediatrics 141: e20171811.
'Lactobacillus reuteri to treat infant colic: a meta-analysis ' () 141 Pediatrics : e20171811.
-
Taylor, A., Hale, J., Wiltschut, J., Lehmann, H., Dunstan, J.A. and Prescott, S.L., 2006. Evaluation of the effects of probiotic supplementation from the neonatal period on innate immune development in infancy. Clinical and Experimental Allergy 36: 1218-1226.
'Evaluation of the effects of probiotic supplementation from the neonatal period on innate immune development in infancy ' () 36 Clinical and Experimental Allergy : 1218 -1226.
-
Toiyama, Y., Araki, T., Yoshiyama, S., Hiro, J., Miki, C. and Kusunoki, M., 2006. The expression patterns of Toll-like receptors in the ileal pouch mucosa of postoperative ulcerative colitis patients. Surgery Today 36: 287-290.
'The expression patterns of Toll-like receptors in the ileal pouch mucosa of postoperative ulcerative colitis patients ' () 36 Surgery Today : 287 -290.
-
Török, H.P., Bellon, V., Konrad, A., Lacher, M., Tonenchi, L., Siebeck, M., Brand, S. and De Toni, E.N., 2017. Functional Toll-Like Receptor (TLR)2 polymorphisms in the susceptibility to inflammatory bowel disease. PLoS One 12: e0175180.
'Functional Toll-Like Receptor (TLR)2 polymorphisms in the susceptibility to inflammatory bowel disease ' () 12 PLoS One : e0175180.
-
Van der Sloot, K.W.J., Amini, M., Peters, V., Dijkstra, G. and Alizadeh, B.Z., 2017. Inflammatory bowel diseases: review of known environmental protective and risk factors involved. Inflammable Bowel Disease 23: 1499-1509.
'Inflammatory bowel diseases: review of known environmental protective and risk factors involved ' () 23 Inflammable Bowel Disease : 1499 -1509.
-
Wessel, M.A., Cobb, J.C., Jackson, E.B., Harris Jr., G.S. and Detwiler, A.C., 1954. Paroxysmal fussing in infancy, sometimes called colic. Pediatrics 14: 421-435.
'Paroxysmal fussing in infancy, sometimes called colic ' () 14 Pediatrics : 421 -435.
-
Wu, R.Y., Pasyk, M., Wang, B., Forsythe, P., Bienenstock, J., Mao, Y.K., Sharma, P., Stanisz, A.M. and Kunze, W.A., 2013. Spatiotemporal maps reveal regional differences in the effects on gut motility for Lactobacillus reuteri and rhamnosus strains. Neurogastroenterology and Motility 25: e205-e214.
'Spatiotemporal maps reveal regional differences in the effects on gut motility for Lactobacillus reuteri and rhamnosus strains ' () 25 Neurogastroenterology and Motility : e205 -e214.
-
Xu, M., Wang, J., Wang, N., Sun, F., Wang, L. and Liu, X.H., 2015. The efficacy and safety of the probiotic bacterium Lactobacillus reuteri DSM 17938 for infantile colic: a meta-analysis of randomized controlled trials. PLoS One 10: e0141445.
'The efficacy and safety of the probiotic bacterium Lactobacillus reuteri DSM 17938 for infantile colic: a meta-analysis of randomized controlled trials ' () 10 PLoS One : e0141445.
-
Yao, P., Tan, F., Gao, H., Wang, L., Yang, T. and Cheng, Y., 2017. Effect of probiotics on Toll-like receptor expression in ulcerative colitis rats induced by 2,4,6-trinitro benzene sulfonic acid. Molecular Medicine Reports 15: 1973-1980.
'Effect of probiotics on Toll-like receptor expression in ulcerative colitis rats induced by 2,4,6-trinitro benzene sulfonic acid ' () 15 Molecular Medicine Reports : 1973 -1980.
-
Zhang, M., Sun, K., Wu, Y., Yang, Y., Tso, P. and Wu, Z., 2017. Interactions between intestinal microbiota and host immune response in inflammatory bowel disease. Frontiers in Immunology 8: 942.
'Interactions between intestinal microbiota and host immune response in inflammatory bowel disease ' () 8 Frontiers in Immunology : 942.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 224 | 175 | 34 |
| PDF Views & Downloads | 228 | 203 | 37 |
Regulatory T cells and Toll-like receptor 2 and 4 mRNA expression in infants with colic treated with Lactobacillus reuteri DSM17938
In: Beneficial MicrobesSearch for other papers by F. Savino in
Current site
Google Scholar
PubMed
Search for other papers by I. Galliano in
Current site
Google Scholar
PubMed
Search for other papers by M. Garro in
Current site
Google Scholar
PubMed
Search for other papers by A. Savino in
Current site
Google Scholar
PubMed
Search for other papers by V. Daprà in
Current site
Google Scholar
PubMed
Search for other papers by P. Montanari in
Current site
Google Scholar
PubMed
Search for other papers by M. Bergallo in
Current site
Google Scholar
PubMed
Regulatory T cells induce immune homeostasis and the expression of Toll like receptors (TLRs); subsequent inflammatory cytokine release may be involved. Recent studies have shown a microbial imbalance in the gut of colicky infants (with a prevalence of gram-negative bacteria, such as Escherichia coli), and accumulating evidence has shown the efficacy of a probiotic (Lactobacillus reuteri) in breastfed subjects, but the underlying mechanism remains undefined. The study enrolled 59 infants younger than 60 days, of whom 34 subjects had colic and 25 were healthy controls. With a double-blind, placebo-controlled randomised study performed in our unit from October 2016 to July 2017, infants with colic were randomly assigned to receive oral daily L. reuteri DSM17938 (1×108 cfu) or placebo for 28 days. Peripheral blood was collected to assess the expression of FoxP3, TLR2 and TLR4 mRNA using real-time TaqMan RT-PCR at baseline and after the study period. Our findings showed increased mRNA expression of the transcription factor forkhead box P3 (FoxP3) in infants treated with L. reuteri DSM 17938 for 28 days (P<0.009) and increased TLR2 and TLR4 mRNA expression in both treated and placebo subjects. After L. reuteri administration for 28 days in infants with colic, we observed a significant decrease in daily crying time (302.3±19.86 min/day on day 0 vs 76.75±22.15 min/day on day 28, P=0.001). This study provides evidence that the observed increase in FoxP3 expression and reduction in crying time might be responses to probiotic treatment, while the increase in TLR2 and TLR4 mRNA expression might be related to age. Exploiting these new findings may lead to an unprecedented level of therapeutic control over immune tolerance using probiotics.