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The Effect of Preparation Techniques on Sem-Imaging of Pit Membranes

In: IAWA Journal
Authors:
Steven Jansen
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Annelies Pletsers
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Yuzou Sano
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Online Publication Date:
01 Jan 2008
Free access

This paper examines the effects of 30 preparation techniques on SEMimages of pit membranes in vessels and tracheids from the two latest growth rings in twigs of Pinus wallichiana, Fraxinus americana and Laurus nobilis. Most variation observed is due to coating and treatment with chemical solutions, such as acetone, ethanol and hydrogen peroxide. The effects of chemicals appear to be associated with the thickness of the pit membrane, resulting in an increased density and diameter of pores in pit membranes of F. americana and L. nobilis and an almost complete dissolution of the porous margo in P. wallichiana. Although different protocols offer advantages for different species, the following methods are recommended: (1) the use of fresh material, (2) air-drying without any chemical treatment, (3) splitting of dried samples, (4) vacuum evaporation with platinum, and (5) SEM-imaging at an accelerating voltage below 5 kV.

Title:
The Effect of Preparation Techniques on Sem-Imaging of Pit Membranes
Article Type:
Research Article
DOI:
https://doi.org/10.1163/22941932-90000177
Language:
English
Pages:
161–178
Keywords:
Fraxinus americana; Laurus nobilis; Pinus wallichiana; margo; pit membrane; scanning electron microscopy
In:
IAWA Journal
In:
Volume 29: Issue 2
Publisher:
Brill
E-ISSN:
2294-1932
Print ISSN:
0928-1541
Subjects:
Botany, Biology

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