Ice nucleation in stems of trees and shrubs with different frost resistance

in IAWA Journal

Abstract

In this study, the ice nucleation activity (INA) and ice nucleation temperature (INT) as well as extracellular ice formation within the bark were determined for three woody species with different degrees of frost resistance, Betula nana, Betula albosinensis and Castanea sativa. Current-year stems and at least 2-year old stems of B. nana and C. sativa as well as current-year stems of B. albosinensis were compared, during summer (non-acclimated state) and winter (acclimated state), to evaluate possible ontogenetic and seasonal differences. Acclimated plant parts of the selected species revealed nearly similar results, with an INT from -7.52 to -8.43°C. The current-year stems of B. nana had a somewhat higher INT than the older stems. Microscopic analysis showed that extra-cellular ice formation occurred in the intercellular spaces within the bark of stems of B. nana, B. albosinensis and C. sativa. Size of the intercellular spaces of the bark were species-specific, and B. nana showed the largest intercellular space volume. While freezing behavior and extracellular ice formation thus followed principally the same pattern in all considered species, B. nana is obviously capable of dealing with large masses of extracellular ice which accumulate over extended periods of frost, making B. nana capable of protecting living tissue in colder regions from freezing damage.

  • Améglio T Cochard H Ewers FW. 2001. Stem diameter variations and cold hardiness in walnut trees. J. Experim. Bot. 52 (No. 364): 21352142.

    • Search Google Scholar
    • Export Citation
  • Angyalossy V Pace MR Evert RF Marcati CR Oskolski AA Terrazas T Kotina E Lens F Mazzoni-Viveiros SC Angeles G Machado SR Crivellaro A Rao KS Junikka L Nikolaeva N Baas P . 2016. IAWA List of Microscopic Bark Features. IAWA Journal 37 (4): 517615.

    • Search Google Scholar
    • Export Citation
  • Coombes AJ . 2012. Blätter und ihre Bäume: Haupt Berne.

  • de Groot WJ Thomas PA Wein RW . 1997. Betula nana L. and Betula glandulosa Michx. J. Ecol. 85: 241264.

  • Eurich L Schott R Wagner A Roth-Nebelsick A Ehlers W . 2016. Fundamentals of heat and mass transport in frost-resistant plant tissues. In: Knippers JNickel KGSpeck T (eds.) Biomimetic research for architecture and building construction. Biological design and integrative structures. Bd. 8. Springer Biologically-Inspired Systems 9: 97108. SpringerBerlin.

    • Search Google Scholar
    • Export Citation
  • Kishimoto T Sekozawa Y Yamazaki H Murakawa H Kuchitsu K Ishikawa M . 2014a. Seasonal changes in ice nucleation activity in blueberry stems and effects of cold treatments in vitro. Environm. Experim. Bot. 106: 1323.

    • Search Google Scholar
    • Export Citation
  • Kishimoto T Yamazaki H Saruwatari A Murakawa H Sekozawa Y Kuchitsu K et al. 2014b. High ice nucleation activity located in blueberry stem bark is linked to primary freeze initiation and adaptive freezing behaviour of the bark. AoB Plants 6. DOI: .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Mazur P. 1969. Freezing injury in plants. Annl. Rev. Plant Physiol. 20: 419448.

  • McCully ME Canny MJ Huang CX . 2004. The management of extracellular ice by petioles of frost-resistant herbaceous plants. Ann. Bot. 94 (5): 665674.

    • Search Google Scholar
    • Export Citation
  • Neuner G Xu B Hacker J . 2010. Velocity and pattern of ice propagation and deep super-cooling in woody stems of Castanea sativa, Morus nigra and Quercus robur measured by IDTA. Tree Physiol. 30 (8): 10371045.

    • Search Google Scholar
    • Export Citation
  • Pearce R. 2001. Plant freezing and damage. Ann. Bot. 87 (4): 417424.

  • Prillieux M (ed.). 1869. Effet de la gelée sur les plantes. Formation de glaçons dans les tissus des plantes. Bull. Soc. Bot. France 16 (4): 140152.

    • Search Google Scholar
    • Export Citation
  • Rajashekar CB Burke MJ . 1982. Plant cold hardiness and freezing stress. In: Li PH Sakai A (eds.) Mechanisms and crop implications. Vol. 1. Academic PressNew York. pp. 395416.

    • Search Google Scholar
    • Export Citation
  • Roden JS Canny MJ Huang CX Ball MC . 2009. Frist tolerance and ice formation in Pinus radiata needles: ice management by the endodermis and transfusion tissue. Funct. Plant Biol. 36: 180189.

    • Search Google Scholar
    • Export Citation
  • Vali G. 1995. Principles of ice nucleation. In: Lee RWarren GJGusta LV (eds.): Biological ice nucleation and its applications. APS Press: 128.

    • Search Google Scholar
    • Export Citation
  • Welling A. 2003. Overwintering in wood plants: involvement of ABA and dehydrins. Academic Dissertation. Institute of Biotechnology and Department of Biosciences, Division of Genetics, Faculty of Science, University of Helsinki, Finland, Helsinki.

    • Search Google Scholar
    • Export Citation

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