The irrigation and fertilization regime of different varieties of Grevillea in Israel are based on existing knowledge for growing various varieties of the Proteaceae family for production of cut flowering branches. However, growers face problems in cultivating Grevillea “Spiderman,” such as leaf chlorosis, prolonged growth until flowering, and reduced quality of cut flowering branches. The present study aimed to examine whether these problems stem from deficiency or excess of Fe, Mn, Zn, P, and Mg, focusing on the effect of these nutrients on growth, flowering, and appearance of visual leaf symptoms and on yield, quality, and vase life longevity of cut flowering branches. The nutrient treatments significantly affected plant development and flowering. Increasing the Fe concentration from 1 to 2 or 3 mg l–1 resulted in improved leaf color, from slightly yellow to dark green. The combination of 2 mg l–1 Fe + 1.8 mg l–1 Mn resulted in early flowering, highest yield, and development of long lateral branches. Low levels of P caused in the first year of treatment leaf chlorosis, which was intensified during the third year, resulting in severe yellowing of the flowering branches. Leaf necrosis and tip burn appeared in treatments with high concentrations of Zn, Mn, and Mg. Deficiency of Fe and Mn and high concentration of P and Mg led to the development of a large number of branches without flowers. The optimal fertilization treatment that yielded the highest quality of flowering branches after harvest was 2 mg l–1 Fe. Branches of this treatment had green foliage at harvest and the longest vase life (10 days) following the recommended postharvest treatment and air transport simulation. Based on the findings of the present research, it can be concluded that the problems in the cultivation of G. “Spiderman,” such as leaf chlorosis, delayed flowering, and reduced quality of flowering branches, result from improper fertilization.