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Morphological and molecular characterisation of three species of Paratylenchus Micoletzky, 1922 (Tylenchida: Paratylenchidae) from China, with a first description of the male P. rostrocaudatus

In: Nematology
Authors:
Kan Zhuo Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, P.R. China

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Xingtong Liu Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, P.R. China

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Ye Tao Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, P.R. China

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Honghong Wang Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, P.R. China

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Borong Lin Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, P.R. China

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Jinling Liao Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, P.R. China
Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, P.R. China

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Summary

In a recent survey of plant-parasitic nematodes associated with forest in China, three species of Paratylenchus, including P. aculentus, P. leptos and P. rostrocaudatus, were found. Paratylenchus leptos and P. rostrocaudatus are new records for China and the male of P. rostrocaudatus is described for the first time. Morphological descriptions, morphometrics and light microscopic photos are provided for these three species. Molecular characterisation of these species using ribosomal RNA (rRNA) D2-D3 expansion domains of large subunit (LSU D2-D3), internal transcribed spacer (ITS) and small subunit (SSU) sequences are also given. Except for the ITS sequence of P. aculentus, other sequences were amplified for the first time. Phylogenetic relationships within the genus Paratylenchus are discussed as inferred from the analyses of LSU D2-D3, ITS and SSU.

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