Description of Acrobeles aenigmaticus sp. n. (Rhabditida: Cephalobidae), an unusual species with a poorly developed vulva, from the Kalahari Desert (Ngamiland, Botswana)

In: Nematology

Summary

Acrobeles aenigmaticus sp. n. is described from the North-West District (Ngamiland) of Botswana, which forms part of the Kalahari Desert. This new species is characterised by its body length, ‘double’ cuticle bearing numerous interannular punctations, lateral fields with two longitudinal incisures, lips triangular bearing triangular tines with variation in their morphology, labial probolae bifurcated with prongs bearing triangular tines, pharyngeal corpus 2.8-3.8 times the isthmus length, spermatheca 0.8-1.8 times the body diam., post-vulval sac 1.3-2.2 times the body diam., vagina bent to the left side, vulva located left subventral and very reduced, female tail conoid-elongate, male tail conoid, spicules 24-29 μm and gubernaculum 13-17 μm in length. Molecular characterisation of 18S and 28S rRNA shows the relationship of this species with other species of Acrobeles that also present a ‘double’ cuticle, as well as with other cephalobids (e.g., Cervidellus alutus) with the same characteristic in terms of its cuticle.

  • AbolafiaJ. (2015). A low-cost technique to manufacture a container to process meiofauna for scanning electron microscopy. Microscopy Research and Technique 78771-776. DOI: 10.1002/jemt.22538

    • Search Google Scholar
    • Export Citation
  • AbolafiaJ. & Peña-SantiagoR. (2017). On the identity of Chiloplacus magnus Rashid & Heyns, 1990 and C. insularis Orselli & Vinciguerra, 2002 (Rhabditida: Cephalobidae), two confusable species. Nematology 191017-1034. DOI: 10.1163/15685411-00003104

    • Search Google Scholar
    • Export Citation
  • AndrássyI. (1968). Fauna Paraguayensis. 2. Nematoden aus den Galeriewäldern des Acaray-Flusses. Opuscula Zoologica Budapest 8167-312.

    • Search Google Scholar
    • Export Citation
  • BaermannG. (1917). Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. Geneeskunding Tijdschrift voor Nederlandsch-Indië 57131-137.

    • Search Google Scholar
    • Export Citation
  • BlaxterM.L.De LeyP.GareyJ.R.LiuL.X.ScheldemanP.VierstraeteA.VanfleterenJ.R.MacheyL.Y.DorrisM.FrisseL.M. et al. (1998). A molecular evolutionary framework for the phylum Nematoda. Nature 39271-75. DOI: 10.1038/32160

    • Search Google Scholar
    • Export Citation
  • BoströmS. (1985). Acrobeles oasiensis sp. n. (Nematoda: Cephalobidae) from Tunisia. Nematologia Mediterranea 13123-126.

  • CastresanaJ. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17540-552. DOI: 10.1093/oxfordjournals.molbev.a026334

    • Search Google Scholar
    • Export Citation
  • DarribaD.TaboadaG.L.DoalloR. & PosadaD. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9772. DOI: 10.1038/nmeth.2109

    • Search Google Scholar
    • Export Citation
  • De BruinS. & HeynsJ. (1993). Cephalobidae (Nematoda: Rhabditida) from Botswana. Phytophylactica 251-10.

  • De GrisseA. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen van de Rijksfaculteit Landbouwetenschappen Gent 34351-369.

    • Search Google Scholar
    • Export Citation
  • De LeyP.van de VeldeM.C.MounportD.BaujardP. & CoomansA. (1995). Ultrastructure of the stoma in Cephalobidae, Panagrolaimidae and Rhabditidae, with a proposal for a revised stoma terminology in Rhabditida (Nematoda). Nematologica 41153-182. DOI: 10.1163/003925995X00143

    • Search Google Scholar
    • Export Citation
  • De LeyP.FélixM.-A.FrisseL.M.NadlerS.A.SternbergP.W. & ThomasW.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1591-612. DOI: 10.1163/156854199508559

    • Search Google Scholar
    • Export Citation
  • HallT.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 4195-98.

    • Search Google Scholar
    • Export Citation
  • HeynsJ. (1969). South African species of the genus Acrobeles von Linstow (Nematoda: Cephalobidae). Phytophylactica 157-66.

  • HeynsJ. (1995). Acrobeles farzanae spec. nov. and Seleborca complexa (Thorne) from the West Coast National Park and Rocher Pan Nature Reserve (Nematoda: Cephalobidae). Koedoe 38105-113.

    • Search Google Scholar
    • Export Citation
  • HeynsJ. & HogewindW. (1969). Three new species of Acrobeles von Linstow, 1877 (Nematoda) from southern Africa. Phytophylactica 119-22.

    • Search Google Scholar
    • Export Citation
  • HolovachovO.BoströmS.De LeyI.T.NadlerS.A. & De LeyP. (2009). Description of Penjatinema novaezeelandiae sp. n. (Rhabditida: Cephalobidae) from New Zealand – a second species of a rare genus. Journal of Nematode Morphology and Systematics 127-18.

    • Search Google Scholar
    • Export Citation
  • HolovachovO.BoströmS.De LeyI.T.RobinsonC.Mundo-OcampoM. & NadlerS.A. (2013). Morphology, molecular characterisation and systematic position of the genus Cynura Cobb, 1920 (Nematoda: Plectida). Nematology 15611-627. DOI: 10.1163/15685411-00002706

    • Search Google Scholar
    • Export Citation
  • HuelsenbeckJ.P. & RonquistF. (2001). MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17754-755. DOI: 10.1093/bioinformatics/17.8.754

    • Search Google Scholar
    • Export Citation
  • KoohkanM.ShokoohiE. & MullinP. (2015). Phylogenetic relationships of three families of the suborder Mononchina Kirjanova & Krall, 1969 inferred from 18S rDNA. Nematology 171113-1125. DOI: 10.1163/15685411-00002928

    • Search Google Scholar
    • Export Citation
  • KumarS.StecherG. & TamuraK. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Molecular Biology and Evolution 331870-1874. DOI: 10.1093/molbev/msw054

    • Search Google Scholar
    • Export Citation
  • NadlerS.A.De LeyP.Mundo-OcampoM.SmytheA.B.StockS.P.BumbargerD.AdamsB.J.Tandingan De LeyI.HolovachovO. & BaldwinJ.G. (2006). Phylogeny of Cephalobina (Nematoda): molecular evidence for recurrent evolution of probolae and incongruence with traditional classifications. Molecular Phylogenetics and Evolution 40696-711. DOI: 10.1016/j.ympev.2006.04.005

    • Search Google Scholar
    • Export Citation
  • Rambaut A. (2014). Figtree a graphical viewer of phylogenetic trees. Available from: http://tree.bio.ed.ac.uk/software/figtree.

  • RashidF.HeynsJ. & CoomansA. (1990). Species of Seleborca Andrássy, 1985 from South West Africa/Namibia (Nematoda: Cephalobidae). Phytophylactica 2251-62.

    • Search Google Scholar
    • Export Citation
  • RonquistF. & HuelsenbeckJ. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 191572-1574. DOI: 10.1093/bioinformatics/btg180

    • Search Google Scholar
    • Export Citation
  • ShahinaF. & De LeyP. (1997). Two new species of Cephalobidae from Valle de la Luna, Argentina, and observations on the genera Acrobeles and Nothacrobeles (Nematoda: Rhabditida). Fundamental and Applied Nematology 20329-347.

    • Search Google Scholar
    • Export Citation
  • ShokoohiE.MaraisM.FourieH. & PanahiH. (2018). Morphological and molecular characterization of three Helicotylenchus species from Iran. Zootaxa 4388207-220. DOI: 10.11646/zootaxa.4388.2.4

    • Search Google Scholar
    • Export Citation
  • SiddiqiM.R. (1993). Nematodes of tropical rainforests: 2. Five new genera and eight new species of cephalobs. Afro-Asian Journal of Nematology 3212-225.

    • Search Google Scholar
    • Export Citation
  • SmytheA.B. & NadlerS.A. (2006). Molecular phylogeny of Acrobeloides and Cephalobus (Nematoda: Cephalobidae) reveals paraphyletic taxa and recurrent evolution of simple labial morphology. Nematology 8819-836. DOI: 10.1163/156854106779799178

    • Search Google Scholar
    • Export Citation
  • ThorneG. (1925). The genus Acrobeles von Linstow, 1887. Transactions of the American Microscopical Society 44171-210.

  • van MegenH.van den ElsenS.HoltermanM.KarssenG.MooymanP.BongersT.HolovachovO.BakkerJ. & HelderJ. (2009). A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences. Nematology 11927-950. DOI: 10.1163/156854109X456862

    • Search Google Scholar
    • Export Citation

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