New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran
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Summary
During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.
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-
Álvarez-Ortega, S. & Peña-Santiago, R. (2016). Aporcella charidemiensis sp. n. (Dorylaimida: Aporcelaimidae) from the southern Iberian Peninsula, with comments on the phylogeny of the genus. Nematology 18, 811-821. DOI: 10.1163/15685411-00002995
-
Andrássy, I. (1954). Revision der Gattung Tylenchus Bastian, 1865. Acta Zoologica Hungarica 1, 5-42.
-
Andrássy, I. (1959). Freilebende Nematoden aus Rumänien. Annales Universitatis Scientiarum Budapestinensis de Rolando Eötvös nominatae. Sectio Biologica 2, 3-27.
-
Andrássy, I. (1968). Fauna Paraguayensis. 2. Nematoden aus den Galeriewäldern des Acaray Flusses. Opuscula Zoologica Budapest 8, 167-315.
-
Andrássy, I. (1980). The genera and species of the family Tylenchidae Orley, 1880 (Nematoda). The genera Aglenchus (Andrássy, 1954) Meyl, 1961, Miculenchus Andrássy, 1959, and Polenchus gen. n. Acta Zoologica Hungarica 26, 1-20.
-
Andrássy, I. (1981). The genera and species of the family Tylenchidae Orley, 1880 (Nematoda). The genus Malenchus Andrassy, 1968. Acta Zoologica Hungarica 27, 1-47.
-
Andrássy, I. (2007). Free-living nematodes of Hungary II (Nematoda errantia). In: Pedozoologica Hungarica, 4 (Series Editors: Csuzdi, C. & Mahunka, S.). Budapest, Hungary, Hungarian Natural History Museum and Systematic Research Group of the Hungarian Academy of Sciences.
-
Brzeski, M. (1996). Variability of Miculenchus salvus Andrássy, 1959 (Nematoda: Tylenchidae). Nematologica 42, 462-465. DOI: 10.1163/004525996X00064
-
Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540-552. DOI: 10.1093/oxfordjournals.molbev.a026334
-
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772. DOI: 10.1038/nmeth.2109
-
De Grisse, A.T. (1969). Redescription et modification de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen Gent 34, 351-369.
-
de Man, J.G. (1880). Die einheimischen, frei in der reinen Erde und im süssenWasser lebenden Nematoden. Vorläufiger Bericht und descriptiv-systematischer Theil. Tijdschrift de Nederlandsche Dierkundige Vereeniging 5, 1-104.
-
Ebsary, B. (1986). Ceramotylenchus tesselatus n. gen., n. sp. and Pleurotylenchus minor n. sp. (Nematoda: Tylenchida) from Alabama. Canadian Journal of Zoology 64, 238-242.
-
Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792-1797. DOI: 10.1093/nar/gkh340
-
Geraert, E. (2008). The Tylenchidae of the world: identification of the family Tylenchidae (Nematoda). Ghent, Belgium, Academia Press, pp. 401-407.
-
Geraert, E. & Raski, D.J. (1986). Three new species of Basirienchus g. n. from Southern Chile compared with Campbellenchus, Neothada and Basiria (Nemata: Tylenchidae). Nematologica 31, 266-288. DOI: 10.1163/187529285X00364
-
Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J. & Helder, J. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792-1800. DOI: 10.1093/molbev/msl044
-
Huson, D.H. & Scornavacca, C. (2012). Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks. Systematic Biology 61, 1061-1067. DOI: 10.1093/sysbio/sys062
-
Khan, S.H. (1964). Sakia typica n. gen., n. sp. (Nematoda: Neotylenchidae) from North India. Proceedings of the 51st and 52nd Sessions, Indian Science Congress Association. Part III, p. 467.
-
Maggenti, A., Luc, M., Raski, D., Fortuner, R. & Geraert, E. (1988). A reappraisal of Tylenchina (Nemata). XI: list of generic and supra-generic taxa, with their junior synonyms. Revue de Nématologie 11, 177-188.
-
Maqbool, M.A. & Shahina, F. (1985). Two new and two known species of the genus Malenchus Andrássy, 1968 (Nematoda; Tylenchidae) from Pakistan. Pakistan Journal of Nematology 3, 1-7.
-
Massey, C.L. (1969). New species of tylenchs associated with bark beetles in New Mexico and Colorado. Proceedings of the Helminthological Society of Washington 36, 43-52.
-
Nunn, G.B. (1992). Nematode molecular evolution. Ph.D. Dissertation, University of Nottingham, Nottingham, UK.
-
Örley, L. (1880). Az anguillulidák magánrajza. Természetrajzi Füzetek 4, 1-165.
-
Panahandeh, Y., Pourjam, E., Roshan-Bakhsk, A. & Pedram, M. (2018a). First record of the genus Sakia Khan, 1964 (Nematoda: Tylenchidae) from Iran, with proposal of Sakia arboris n. sp. Nematology 20, 449-460. DOI: 10.1163/15685411-00003150
-
Panahandeh, Y., Abolafia, J., Pourjam, E., Jahanshahi-Afshar, F., Giblin-Davis, R.M. & Pedram, M. (2018b). Morphological and molecular characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran. Journal of Nematology 50, 343-354.
-
Pedram, M. (2017). Cryptaphelenchus varicaudatus n. sp. (Rhabditida: Ektaphelenchinae) from Tehran Province, Iran. Journal of Nematology 49, 223-230.
-
Qing, X., Sánchez-Monge, A., Janssen, T., Couvreur, M. & Bert, W. (2016). Description of Malenchus sexlineatus n. sp., new records of three known species of Malenchus Andrássy, 1968 (Nematoda: Tylenchidae) and notes on amphidial aperture development. Nematology 18, 155-174. DOI: 10.1163/15685411-00002951
-
Qing, X., Pereira, T.J., Slos, D., Couvreur, M. & Bert, W. (2018). A new species of Malenchus (Nematoda: Tylenchomorpha) with an updated phylogeny of the Tylenchidae. Nematology 20, 815-836. DOI: 10.1163/15685411-00003177
-
Rambaut, A. & Drummond, A.J. (2009). Tracer version 1.5 (computer program), available online at http://beast.bio.ed.ac.uk/.
-
Raski, D.L. & Geraert, E. (1984). A new species of Miculechus Andrássy, 1959 and further notes on M. salvus (Nematoda: Tylenchidae). Nematologica 30, 419-428. DOI: 10.1163/187529284X00310
-
Ronquist, F. & Huelsenbeck, J.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180
-
Siddiqi, M.R. (1979). Seven new species in a new nematode subfamily Duosulciinae (Tylenchidae), with proposals for Duosulcius gen. n., Zanenchus gen. n. and Neomalenchus gen. n. Nematologica 25, 215-236. DOI: 10.1163/187529279X00244
-
Siddiqi, M.R. (1986). Tylenchida parasites of plants and insects. Farnham Royal, UK, Commonwealth Agricultural Bureaux.
-
Siddiqi, M.R. (2000). Tylenchida parasites of plants and insects, 2nd edition. Wallingford, UK, CAB International.
-
Silvestro, D. & Michalak, I. (2012). RAxML GUI: a graphical frontend for RAxML. Organisms Diversity and Evolution 12, 335-337.
-
Soleymanzadeh, M., Pedram, M., Pourjam, E. & Álvarez-Ortega, S. (2016). Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family. Nematology 18, 987-998. DOI: 10.1163/15685411-00003010
-
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729. DOI: 10.1093/molbev/mst197
-
Thorne, G. & Malek, R.B. (1968). Nematodes of the northern Great Plains. Part I. Tylenchida (Nemata: Secernentea). South Dakota Agricultural Experiment Station Technical Bulletin 31, 1-111.
-
Wanless, F. & David, R. (1997). Miculenchus salvus (= Zanenchus salmae n. syn.) from Loch Ness, Scotland (Nematoda: Tylenchidae). Nematologica 43, 275-281. DOI: 10.1163/005025997X00030
-
Whitehead, A.G. & Hemming, J.R. (1965). A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 55, 25-38.
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New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran
In: NematologySearch for other papers by Yousef Panahandeh in
Current site
Google Scholar
PubMed
Search for other papers by Ebrahim Pourjam in
Current site
Google Scholar
PubMed
Search for other papers by Sergio Álvarez-Ortega in
Current site
Google Scholar
PubMed
Search for other papers by Farahnaz Jahanshahi Afshar in
Current site
Google Scholar
PubMed
Search for other papers by Majid Pedram in
Current site
Google Scholar
PubMed
Summary
During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 530 | 174 | 10 |
| Full Text Views | 108 | 2 | 0 |
| PDF Views & Downloads | 51 | 4 | 0 |
