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Molecular characterisation and phylogenetic position of Hemicycliophora labiata Colbran, 1960, from turfgrass in Korea, with comments on its morphology

In: Nematology
Authors:
Abraham Okki Mwamula Department of Ecological Science, Kyungpook National University, Sangju, 37224, South Korea
Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, Kampala, 7062, Uganda

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Yeong Ho Kim School of Ecological Environment and Tourism, Kyungpook National University, Sangju, 37224, South Korea

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Heebeen Na Department of Ecological Science, Kyungpook National University, Sangju, 37224, South Korea

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Hyeon Jeong An Department of Ecological Science, Kyungpook National University, Sangju, 37224, South Korea

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Young Ho Kim School of Ecological Environment and Tourism, Kyungpook National University, Sangju, 37224, South Korea

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Dong Woon Lee Department of Ecological Science, Kyungpook National University, Sangju, 37224, South Korea

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Summary

Hemicycliophora labiata was reported from the rhizosphere of Poa pratensis and for the first time in Korea. Females and juveniles are molecularly characterised and morphological and morphometric descriptions supplied. Species identification was made using an integrative approach considering morphological characteristics and the results of the analyses of the D2-D3 expansion segment of 28S rRNA, ITS1-5.8S-ITS2 of rRNA and the partial COI gene sequences. The phylogenetic position of H. labiata is resolved after recent misidentifications due to the existence of a wide morphological plasticity within the genus. Females and juveniles from Korea conform to the original description and also to subsequent species descriptions from Australia, South Africa, Sri Lanka, and India. Despite the close morphological similarities with H. typica de Man, 1921, the two species can be adequately differentiated based on molecular data. This is the first molecular characterisation of H. labiata. The use of molecular markers in species delimitation continues to explore the existence of species complexes, thus providing a better understanding of the unresolved species biodiversity within this speciose genus.

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