Bio-protective effect of a root-nodulating Rhizobium etli strain in common bean (Phaseolus vulgaris) against Meloidogyne incognita and Radopholus similis in an in vitro autotrophic tripartite culture system

In: Nematology
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  • 1 Pro Terra Agro, Bijlokstraat 144, B-3020 Herent, Belgium
  • 2 Livelihoods and Food Security Fund (LIFT, UNOPS), Mayangone Township, Yangon, Myanmar
  • 3 Faculty of Agriculture, University of Mauritius, Reduit 80837, Mauritius
  • 4 Department of Biological Sciences, College of Science and Mathematics, University of Southern Mindanao, 9407 Kabacan, Cotabato, Philippines
  • 5 Laboratory of Tropical Crop Improvement, Department of Biosystems, Faculty of Bioscience Engineering, University of Leuven (KULeuven), W. De Croylaan 42, B-3001 Leuven, Belgium
  • 6 International Institute of Tropical Agriculture (IITA), Arusha, Tanzania
  • 7 Soil Service of Belgium, W. De Croylaan 48, B-3001 Heverlee, Belgium
  • 8 Department of Biology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
  • 9 Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa

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Summary

The bio-protective effect of a root-nodulating strain (CNPAF 512) of the nitrogen-fixing rhizobium, Rhizobium etli, against both a sedentary (Meloidogyne incognita) and a migratory (Radopholus similis) endoparasitic nematode in common bean (Phaseolus vulgaris) was examined using an in vitro autotrophic tripartite culture system. Two in vitro assays were carried out with each of the nematode species. Each assay consisted of two treatments: the plants were either inoculated with the rhizobial strain or remained non-inoculated (control plants). To examine the effect of either pre- or simultaneous inoculation of the rhizobial strain on the reproduction of M. incognita and R. similis, one assay was carried out in which the nematodes were inoculated 3 weeks after rhizobial inoculation while another assay was carried out in which the nematodes were inoculated simultaneously with the rihizobial strain. Both pre-inoculation and simultaneous inoculation with R. etli CNPAF 512 significantly suppressed the reproduction of both M. incognita and R. similis.

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