Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market
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Ochratoxin A (OTA) belongs among the most frequently occurring mycotoxins in coffee. In order to investigate its contamination levels in products currently available in the market, a broad set of coffee samples (103 in total) collected between 2016 and 2018 in the Czech Republic was investigated. Aqueous-methanolic extracts purified by using immunoaffinity columns were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (U-HPLC-MS/MS). The undertaken study revealed a relatively low OTA contamination of roasted coffee (in the range 0.2-2.5 μg/kg with the mean concentration of 0.6 μg/kg, and 71% of positive samples). The roasted coffee samples did not exceed the maximum limit of 5 μg/kg set by 1881/2006/EC. With regard to instant coffee samples, OTA concentrations were considerably higher. All the samples were positive, with a mean concentration of 2.9 μg/kg (ranging from 0.6 to 12.8 μg/kg, with 100% of positive samples). One of the analysed samples of instant coffee even exceeded the maximum limit of 10 μg/kg (1881/2006/EC). The study further revealed a relatively high incidence of 14-(R)-OTA, stereoisomer of OTA (14-(S)-OTA), originating as its main degradation product. Its identity was confirmed by high resolution mass spectrometry (HRMS/MS). Most of the samples positive for OTA were also positive for this diastereoisomer, with signal intensities of approx. one-third to one half of the signal of 14-(S)-OTA.
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Supplementary Materials
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Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market
In: World Mycotoxin JournalSearch for other papers by P. Jonatova in
Current site
Google Scholar
PubMed
Search for other papers by Z. Dzuman in
Current site
Google Scholar
PubMed
Search for other papers by N. Prusova in
Current site
Google Scholar
PubMed
Search for other papers by J. Hajslova in
Current site
Google Scholar
PubMed
Search for other papers by M. Stranska-Zachariasova in
Current site
Google Scholar
PubMed
Ochratoxin A (OTA) belongs among the most frequently occurring mycotoxins in coffee. In order to investigate its contamination levels in products currently available in the market, a broad set of coffee samples (103 in total) collected between 2016 and 2018 in the Czech Republic was investigated. Aqueous-methanolic extracts purified by using immunoaffinity columns were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (U-HPLC-MS/MS). The undertaken study revealed a relatively low OTA contamination of roasted coffee (in the range 0.2-2.5 μg/kg with the mean concentration of 0.6 μg/kg, and 71% of positive samples). The roasted coffee samples did not exceed the maximum limit of 5 μg/kg set by 1881/2006/EC. With regard to instant coffee samples, OTA concentrations were considerably higher. All the samples were positive, with a mean concentration of 2.9 μg/kg (ranging from 0.6 to 12.8 μg/kg, with 100% of positive samples). One of the analysed samples of instant coffee even exceeded the maximum limit of 10 μg/kg (1881/2006/EC). The study further revealed a relatively high incidence of 14-(R)-OTA, stereoisomer of OTA (14-(S)-OTA), originating as its main degradation product. Its identity was confirmed by high resolution mass spectrometry (HRMS/MS). Most of the samples positive for OTA were also positive for this diastereoisomer, with signal intensities of approx. one-third to one half of the signal of 14-(S)-OTA.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 286 | 231 | 18 |
| Full Text Views | 31 | 2 | 0 |
| PDF Views & Downloads | 23 | 3 | 0 |
