The purpose of this study was to provide data on the presence of ochratoxin A (OTA) in artisan salamis produced in the Campania and Marche regions (Italy). For this aim, 96 different salamis were purchased from farms and small salami factories. Analysis were carried out firstly on the casings of all samples, and in a second step, in the outer and inner edible parts of those samples whose casings were found positive for OTA at concentration levels above the Italian guideline value established for OTA in pork meat (1 μg/kg). The analytical method, based on a sample preparation procedure with immunoaffinity columns, together with analysis by LC-MS/MS, has guaranteed average recoveries between 79.4 and 89.0%, limits of detection (LOD) and quantification (LOQ) of 0.10 and 0.25 μg/kg, respectively. OTA was detected in 25.0% of the analysed casings (24 samples) at concentration ranging from 0.25 to 98.52 μg/kg. Ten of these samples were from the same production plant in which an additional sampling was carried out, and where a problem of environmental contamination by ochratoxigenic moulds probably exists. The edible parts were mostly uncontaminated, except in 3 samples, 2 of which showed OTA contamination levels above 1 μg/kg. The presence of OTA on the casing does not seem to be cause for alarm about the safety of this type of product but it should in any case be monitored. Before slicing the meat for consumption, it is always good practice to peel the casing even if reported as edible on salami label.
Altafini, A., Armorini, S., Zaghini, A., Sardi, L. and Roncada, P., 2017. Tissue distribution of ochratoxin A in pigs after administration of two-levels contaminated diets. World Mycotoxin Journal 10: 263-272.
'Tissue distribution of ochratoxin A in pigs after administration of two-levels contaminated diets ' () 10 World Mycotoxin Journal : 263 -272.
Altafini, A., Fedrizzi, G. and Roncada, P., 2019. Occurrence of ochratoxin A in typical salami produced in different regions of Italy. Mycotoxin Research 35: 141-148.
'Occurrence of ochratoxin A in typical salami produced in different regions of Italy ' () 35 Mycotoxin Research : 141 -148.
Armorini, S., Altafini, A., Zaghini, A. and Roncada, P., 2016. Ochratoxin A in artisan salami produced in Veneto (Italy). Food Additives and Contaminants Part B 9: 9-14.
'Ochratoxin A in artisan salami produced in Veneto (Italy) ' () 9 Food Additives and Contaminants Part B : 9 -14.
Arslan, B. and Soyer, A., 2018. Effects of chitosan as a surface fungus inhibitor on microbiological, physicochemical, oxidative and sensory characteristics of dry fermented sausages. Meat Science 145: 107-113.
'Effects of chitosan as a surface fungus inhibitor on microbiological, physicochemical, oxidative and sensory characteristics of dry fermented sausages ' () 145 Meat Science : 107 -113.
Associazione Industriali delle Carni e dei Salumi (ASSICA), 2018. Analisi del settore e dati economici 2017. L’Industria delle Carni e dei Salumi. ASSICA, Milan, Italy. Available at:https://tinyurl.com/y85nfat6
Battilani, P., Pietri, A., Giorni, P., Formenti, S., Bertuzzi, T., Toscani, T., Virgili, R. and Kozakiewicz, Z., 2007.Penicillium populations in dry-cured ham manufacturing plants. Journal of Food Protection 70: 975-980.
'Penicillium populations in dry-cured ham manufacturing plants ' () 70 Journal of Food Protection : 975 -980.
Bernáldez, V., Rodríguez, A., Delgado, J., Sánchez-Montero, L., Juan, J. and Córdoba, J.J., 2018. Gene expression analysis as a method to predict OTA accumulation in dry-cured meat products. Food Analytical Methods 11: 2463-2471.
'Gene expression analysis as a method to predict OTA accumulation in dry-cured meat products ' () 11 Food Analytical Methods : 2463 -2471.
Bertuzzi, T., Gualla, A., Morlacchini, M. and Pietri, A., 2013. Direct and indirect contamination with ochratoxin A of ripened pork products. Food Control 34: 79-83.
'Direct and indirect contamination with ochratoxin A of ripened pork products ' () 34 Food Control : 79 -83.
Castegnaro, M., Canadas, D., Vrabcheva, T., Petkova-Bocharova, T., Chernozemsky, I.N. and Pfohl-Leszkowicz, A., 2006. Balkan endemic nephropathy: role of ochratoxins A through biomarkers. Molecular Nutrition and Food Research 50: 519-529.
'Balkan endemic nephropathy: role of ochratoxins A through biomarkers ' () 50 Molecular Nutrition and Food Research : 519 -529.
Comi, G., Chiesa, L., Panseri, S., Orlic, S. and Iacumin, L., 2013. Evaluation of different methods to preventPenicillium nordicum growth on and ochratoxin A production in country-style sausages. World Mycotoxin Journal 6: 411-418.
'Evaluation of different methods to preventPenicillium nordicum growth on and ochratoxin A production in country-style sausages ' () 6 World Mycotoxin Journal : 411 -418.
Comi, G., Orlic, S., Redzepovic, S., Urso, R. and Iacumin, L., 2004. Moulds isolated from Istrian dried ham at the pre-ripening and ripening level. International Journal of Food Microbiology 96: 29-34.
'Moulds isolated from Istrian dried ham at the pre-ripening and ripening level ' () 96 International Journal of Food Microbiology : 29 -34.
Dall’Asta, C., Galaverna, G., Bertuzzi, T., Moseriti, A., Pietri, A., Dossena, A. and Marchelli, R., 2010. Occurrence of ochratoxin A in raw ham muscle, salami and dry-cured ham from pigs fed with contaminated diet. Food Chemistry 120: 978-983.
'Occurrence of ochratoxin A in raw ham muscle, salami and dry-cured ham from pigs fed with contaminated diet ' () 120 Food Chemistry : 978 -983.
European Commission (EC), 2002. Assessment of dietary intake of ochratoxin A by the population of EU member states. Report of the Scientific Cooperation, Task 3.2.7. Directorate-General Health and Consumer Protection. EC, Brussels, Belgium. Available at:http://tinyurl.com/mea7ar5
European Commission (EC), 2006a. Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding, 2006/576/EC. Official Journal of the European Union L 229: 7-9.
European Commission (EC), 2006b. Commission Regulation EC No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Official Journal of the European Union L 70: 12-34.
European Food Safety Authority (EFSA), 2006. Opinion of the Scientific Panel on Contaminants in the Food Chain on a request from the Commission related to ochratoxin A in food. EFSA Journal 365: 1-56.
Fongaro, L., Alamprese, C. and Casiraghi, E., 2015. Ripening of salami: assessment of colour and aspect evolution using image analysis and multivariate image analysis. Meat Science 101: 73-77.
'Ripening of salami: assessment of colour and aspect evolution using image analysis and multivariate image analysis ' () 101 Meat Science : 73 -77.
Gil-Serna, J., Patiño, B., Cortes, L., Gonzalez-Jaen, M.T. and Vazquez, C., 2015.Aspergillus steynii andAspergillus westerdijkiae as potential risk of OTA contamination in food products in warm climates. Food Microbiology 46: 168-175.
'Aspergillus steynii andAspergillus westerdijkiae as potential risk of OTA contamination in food products in warm climates ' () 46 Food Microbiology : 168 -175.
Hald, B., 1991. Porcine nephropathy in Europe. IARC Scientific Publications 115: 49-56.
'Porcine nephropathy in Europe ' () 115 IARC Scientific Publications : 49 -56.
Hassen, W., Abid, S., Achour, A., Creppy, E. and Bacha, H., 2004. Ochratoxin A and β2-microglobulinuria in healthy individuals and in chronic interstitial nephropathy patients in the Centre of Tunisia: a hot spot of ochratoxin A exposure. Toxicology 199: 185-193.
'Ochratoxin A and β2-microglobulinuria in healthy individuals and in chronic interstitial nephropathy patients in the Centre of Tunisia: a hot spot of ochratoxin A exposure ' () 199 Toxicology : 185 -193.
Iacumin, L., Chiesa, L., Boscolo, D., Manzano, M., Cantoni, C., Orlic, S. and Comi, G., 2009. Moulds and ochratoxin A on surfaces of artisanal and industrial dry sausages. Food Microbiology 26: 65-70.
'Moulds and ochratoxin A on surfaces of artisanal and industrial dry sausages ' () 26 Food Microbiology : 65 -70.
Iacumin, L., Manzano, M. and Comi, G., 2012. Prevention ofAspergillus ochraceus growth on and ochratoxin A contamination of sausages using ozonated air. Food Microbiology 29: 229-232.
'Prevention ofAspergillus ochraceus growth on and ochratoxin A contamination of sausages using ozonated air ' () 29 Food Microbiology : 229 -232.
Iacumin, L., Manzano, M., Andyanto, D. and Comi, G., 2017. Biocontrol of ochratoxigenic mouldsAspergillus ochraceus andPenicillium nordicum byDebaryomyces hansenii andSaccharomycopsis fibuligera during speck production. Food Microbiology 62: 188-195.
'Biocontrol of ochratoxigenic mouldsAspergillus ochraceus andPenicillium nordicum byDebaryomyces hansenii andSaccharomycopsis fibuligera during speck production ' () 62 Food Microbiology : 188 -195.
Iacumin, L., Milesi, S., Pirani, S., Comi, G. and Chiesa, L.M., 2011. Ochratoxigenic mold and ochratoxin A in fermented sausages from different areas in Northern Italy: occurrence, reduction or prevention with ozonated air. Journal of Food Safety 31: 538-545.
'Ochratoxigenic mold and ochratoxin A in fermented sausages from different areas in Northern Italy: occurrence, reduction or prevention with ozonated air ' () 31 Journal of Food Safety : 538 -545.
Ingredients Insight, 2018. Growing concern over additives in food. Ingredients Insight, Nottingham, UK Available at:https://tinyurl.com/y8a5dcfy
International Agency for Research on Cancer (IARC), 1993. Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC Monographs on the evaluation of carcinogenic risks to humans. Vol. 56. IARC, Lyon, France.
Joint FAO/WHO Expert Committee on Food Additives (JECFA), 1996. Ochratoxin A. Toxicological evaluation of certain food additives and contaminants. Forty-four meeting of the Joint FAO/WHO Expert Committee on Food Additives. WHO Food Additives Series 35, World Health Organization, Geneva, Switzerland.
Jørgensen, K., 2005. Occurrence of ochratoxin A in commodities and processed food – a review of EU occurrence data. Food Additives and Contaminants, Suppl. 1: 26-30.
'Occurrence of ochratoxin A in commodities and processed food – a review of EU occurrence data ' () 1 Food Additives and Contaminants : 26 -30.
Markov, K., Pleadin, J., Bevardi, M., Vahčić, N., Sokolić-Mihalak, D. and Frece, J., 2013. Natural occurrence of aflatoxin B1, ochratoxin A and citrinin in Croatian fermented meat products. Food Control 34: 312-317.
'Natural occurrence of aflatoxin B1, ochratoxin A and citrinin in Croatian fermented meat products ' () 34 Food Control : 312 -317.
Martín-Sánchez, A.M., Chaves-López, C., Sendra, E., Sayas, E., Fenández-López, J. and Pérez-Álvarez, J.Á., 2011. Lipolysis, proteolysis and sensory characteristics of a Spanish fermented dry-cured meat product salchichón with oregano essential oil used as surface mold inhibitor. Meat Science 89: 35-44.
'Lipolysis, proteolysis and sensory characteristics of a Spanish fermented dry-cured meat product salchichón with oregano essential oil used as surface mold inhibitor ' () 89 Meat Science : 35 -44.
Matos, T.J.S., Jensen, B.B., Bernardo, F.M.A., Barreto, A.H.S. and Hojberg, Ø., 2007. Mycoflora of two types of Portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate. Journal of Food Protection 70: 1468-1474.
'Mycoflora of two types of Portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate ' () 70 Journal of Food Protection : 1468 -1474.
Meftah, S., Abid, S., Dias, T. and Rodrigues, P., 2018. Effect of dry-sausage starter culture and endogenous yeasts onAspergillus westerdijkiae andPenicillium nordicum growth and OTA production. LWT – Food Science and Technology 87: 250-258.
'Effect of dry-sausage starter culture and endogenous yeasts onAspergillus westerdijkiae andPenicillium nordicum growth and OTA production ' () 87 LWT – Food Science and Technology : 250 -258.
Merla, C., Andreoli, G., Garino, C., Vicari, N., Tosi, G., Guglielminetti, M.L., Moretti, A., Biancardi, A., Arlorio, M. and Fabbi, M., 2018. Monitoring of ochratoxin A and ochratoxin-producing fungi in traditional salami manufactured in Northern Italy. Mycotoxin Research 34: 107-116.
'Monitoring of ochratoxin A and ochratoxin-producing fungi in traditional salami manufactured in Northern Italy ' () 34 Mycotoxin Research : 107 -116.
Ministero della Sanità, 1999. CIRCOLARE 9 giugno 1999, n.10. Direttive in materia di controllo ufficiale sui prodotti alimentari: valori massimi ammissibili di micotossine nelle derrate alimentari di origine nazionale, comunitaria e Paesi terzi. Gazzetta Ufficiale 135. Available at:https://tinyurl.com/y8q4uqzg (in Italian)
Mitchell, N.J., Chen, C., Palumbo, J.D., Bianchini, A., Cappozzo, J., Stratton, J., Ryu, D. and Wu, F., 2017. A risk assessment of dietary ochratoxin A in the United States. Food and Chemical Toxicology 100: 265-273.
'A risk assessment of dietary ochratoxin A in the United States ' () 100 Food and Chemical Toxicology : 265 -273.
Monaci, L., Palmisano, F., Matrella, R. and Tantillo, G., 2005. Determination of ochratoxin A at part-per-trillion level in Italian salami by immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. Journal of Chromatography A 1090: 184-187.
'Determination of ochratoxin A at part-per-trillion level in Italian salami by immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection ' () 1090 Journal of Chromatography A : 184 -187.
Pavlović, N.M., 2013. Balkan endemic nephropathy – current status and future perspectives. Clinical Kidney Journal 6: 257-265.
'Balkan endemic nephropathy – current status and future perspectives ' () 6 Clinical Kidney Journal : 257 -265.
Perši, N., Pleadin, J., Kovačević, D., Scortichini, G. and Milone, S., 2014. Ochratoxin A in raw materials and cooked meat products made from OTA-treated pigs. Meat Science 96: 203-210.
'Ochratoxin A in raw materials and cooked meat products made from OTA-treated pigs ' () 96 Meat Science : 203 -210.
Pfohl-Leszkowicz, A., Tozlovanu, M., Manderville, R., Peraica, M., Castegnaro, M. and Stefanovic, V., 2007. New molecular and field evidences for the implication of mycotoxins but not aristolochic acid in human nephropathy and urinary tract tumor. Molecular Nutrition and Food Research 51: 1131-1146.
'New molecular and field evidences for the implication of mycotoxins but not aristolochic acid in human nephropathy and urinary tract tumor ' () 51 Molecular Nutrition and Food Research : 1131 -1146.
Pietri, A., Bertuzzi, T., Gualla, A. and Piva, G., 2006. Occurrence of ochratoxin A in raw ham muscles and in pork products from northern Italy. Italian Journal of Food Science 1: 1-8.
'Occurrence of ochratoxin A in raw ham muscles and in pork products from northern Italy ' () 1 Italian Journal of Food Science : 1 -8.
Pipek, P., Rohlík, B.A., Lojková, A. and Staruch, L., 2010. Suppression of mould growth on dry sausages. Czech Journal of Food Sciences 28: 258-263.
'Suppression of mould growth on dry sausages ' () 28 Czech Journal of Food Sciences : 258 -263.
Pleadin, J., Kudumija, N., Kovačević, D., Scortichini, G., Milone, S. and Kmetič, I., 2016. Comparison of ochratoxin A levels in edible pig tissues and in biological fluids after exposure to a contaminated diet. Mycotoxin Research 32: 145-151.
'Comparison of ochratoxin A levels in edible pig tissues and in biological fluids after exposure to a contaminated diet ' () 32 Mycotoxin Research : 145 -151.
Pleadin, J., Staver, M.M., Vahčić, N., Kovačević, D., Milone, S., Saftić, L. and Scortichini, G., 2015. Survey of aflatoxin B1 and ochratoxin A occurrence in traditional meat products coming from Croatian households and markets. Food Control 52: 71-77.
'Survey of aflatoxin B1 and ochratoxin A occurrence in traditional meat products coming from Croatian households and markets ' () 52 Food Control : 71 -77.
Ribes, S., Fuentes, A., Talens, P. and Barat, J.M., 2018. Prevention of fungal spoilage in food products using natural compounds: a review. Critical Reviews in Food Science and Nutrition 58: 2002-2016.
'Prevention of fungal spoilage in food products using natural compounds: a review ' () 58 Critical Reviews in Food Science and Nutrition : 2002 -2016.
Rizzo, A., Eskola, M. and Atroshi, F., 2002. Ochratoxin A in cereals, foodstuffs and human plasma. European Journal of Plant Pathology 108: 631-637.
'Ochratoxin A in cereals, foodstuffs and human plasma ' () 108 European Journal of Plant Pathology : 631 -637.
Rodríguez, A., Capela, D., Medina, Á., Córdoba, J.J. and Magana, N., 2015. Relationship between ecophysiological factors, growth and ochratoxin A contamination of dry-cured sausage based matrices. International Journal of Food Microbiology 194: 71-77.
'Relationship between ecophysiological factors, growth and ochratoxin A contamination of dry-cured sausage based matrices ' () 194 International Journal of Food Microbiology : 71 -77.
Rodríguez, A., Rodríguez, M., Martín, A., Delgado, J. and Córdoba, J.J., 2012. Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage. Meat Science 90: 728-734.
'Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage ' () 90 Meat Science : 728 -734.
Saggiorato, A.G., Gaio, I., Treichel, H., De Oliveira, D., Cichoski, A.J. and Cansian, R.L., 2012. Antifungal activity of basil essential oil (Ocimum basilicum L.): evaluationin vitro and on an Italian-type sausage surface. Food Bioprocess Technology 5: 378-384.
'Antifungal activity of basil essential oil (Ocimum basilicum L.): evaluationin vitro and on an Italian-type sausage surface ' () 5 Food Bioprocess Technology : 378 -384.
Schiavone, A., Cavallero, C., Girotto, L., Pozzo, L., Antoniazzi, S. and Cavallarin, L., 2008. A survey on the occurrence of ochratoxin A in feeds and sera collected in conventional and organic poultry farms in Northern Italy. Italian Journal of Animal Science 7: 495-503.
'A survey on the occurrence of ochratoxin A in feeds and sera collected in conventional and organic poultry farms in Northern Italy ' () 7 Italian Journal of Animal Science : 495 -503.
Soncu, E.D., Arslan, B., Ertürk, D., Küçükkaya, S., Özdemir, N. and Soyer, A., 2018. Microbiological, physicochemical and sensory characteristics of Turkish fermented sausages sucuk coated with chitosan-essential oils. LWT – Food Science and Technology 97: 198-204.
'Microbiological, physicochemical and sensory characteristics of Turkish fermented sausages sucuk coated with chitosan-essential oils ' () 97 LWT – Food Science and Technology : 198 -204.
Sonjak, S., Ličen, M., Frisvad, J.C. and Gunde-Cimerman, N., 2011. Salting of dry-cured meat – a potential cause of contamination with the ochratoxin A-producing speciesPenicillium nordicum. Food Microbiology 28: 1111-1116.
'Salting of dry-cured meat – a potential cause of contamination with the ochratoxin A-producing speciesPenicillium nordicum ' () 28 Food Microbiology : 1111 -1116.
Spotti, E., Cacchioli, C., Colla, F., Beatrisotti, M. and Zanardi, S., 1999. Growth ofPenicillium verrucosum in model systems based on raw ripened meat products. Ochratoxin A determination and control. Industrie Conserve 74: 113-123.
'Growth ofPenicillium verrucosum in model systems based on raw ripened meat products ' () 74 Industrie Conserve : 113 -123.
Stiborová, M., Arlt, V.M. and Schmeiser, H.H., 2016. Balkan endemic nephropathy: an update on its aetiology. Archives of Toxicology 90: 2595-2615.
'Balkan endemic nephropathy: an update on its aetiology ' () 90 Archives of Toxicology : 2595 -2615.
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The purpose of this study was to provide data on the presence of ochratoxin A (OTA) in artisan salamis produced in the Campania and Marche regions (Italy). For this aim, 96 different salamis were purchased from farms and small salami factories. Analysis were carried out firstly on the casings of all samples, and in a second step, in the outer and inner edible parts of those samples whose casings were found positive for OTA at concentration levels above the Italian guideline value established for OTA in pork meat (1 μg/kg). The analytical method, based on a sample preparation procedure with immunoaffinity columns, together with analysis by LC-MS/MS, has guaranteed average recoveries between 79.4 and 89.0%, limits of detection (LOD) and quantification (LOQ) of 0.10 and 0.25 μg/kg, respectively. OTA was detected in 25.0% of the analysed casings (24 samples) at concentration ranging from 0.25 to 98.52 μg/kg. Ten of these samples were from the same production plant in which an additional sampling was carried out, and where a problem of environmental contamination by ochratoxigenic moulds probably exists. The edible parts were mostly uncontaminated, except in 3 samples, 2 of which showed OTA contamination levels above 1 μg/kg. The presence of OTA on the casing does not seem to be cause for alarm about the safety of this type of product but it should in any case be monitored. Before slicing the meat for consumption, it is always good practice to peel the casing even if reported as edible on salami label.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 171 | 114 | 10 |
| PDF Views & Downloads | 78 | 40 | 0 |