Aflatoxin B1 in the egg chain: monitoring with specific indirect competitive ELISA in northern Paraná, Brazil
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An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.
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-
Abdallah, M.F., Girgin, G., Baydar, T., Krska, R. and Sulyok, M., 2017. Occurrence of multiple mycotoxins and other fungal metabolites in animal feed and maize samples from Egypt using LC-MS/MS. Journal of the Science of Food and Agriculture 97: 4419-4428.https://doi.org/10.1002/jsfa.8293
-
Agência Nacional de Vigilância Sanitária (ANVISA), 2021. Resolução RDC N° 487 de 26 de março de 2021. Available at:https://tinyurl.com/yjcewecf
-
Alshawabkeh, K., Herzallah, S.M., Alkhalaileh, N.I., Abdelqader, A. and Al-Fataftah, A.R.A., 2015. Occurrence of aflatoxin B1 in poultry feed and feed ingredients in Jordan using ELISA and HPLC. American-Eurasian Journal of Toxicological Sciences 7: 316-320.https://doi.org/10.5829/idosi.aejts.2015.7.4.10172
-
Aly, A.S. and Anwer, W., 2009. Effect of naturally contaminated feed with aflatoxins on performance of laying hens and the carryover of aflatoxin B residues in table eggs. Pakistan Journal of Nutrition 8: 181-186.https://doi.org/10.3923/pjn.2009.181.186
-
Amirkhizi, B., Arefhosseini, S.R., Ansarin, M. and Nemati, M., 2015. Aflatoxin B1 in eggs and chicken livers by dispersive liquid-liquid microextraction and HPLC. Food Additives and Contaminants Part B 8: 245-249.https://doi.org/10.1080/19393210.2015.1067649
-
Anfossi, L., Di Nardo, F., Giovannoli, C., Passini, C. and Baggiani, C., 2015. Enzyme immunoassay for monitoring aflatoxins in eggs. Food Control 57: 115-121.https://doi.org/10.1016/j.foodcont.2015.04.013
-
Anjum, M.A., Khan, S.H., Sahota, A.W. and Sardar, R., 2012. Assessment of aflatoxin B1 in commercial poultry feed and feed ingredients. Journal of Animal and Plant Science 22: 268-272.
'Assessment of aflatoxin B1 in commercial poultry feed and feed ingredients ' () 22 Journal of Animal and Plant Science : 268 -272.
-
Associação Brasileira de Proteína Animal (ABPA), 2021. Annual report of the Brazilian Association of Animal Protein. Relatório anual 2021. Available at:https://abpa-br.org/relatorios/
-
Association of Official Analytical Chemists (AOAC), 2016. Guidelines for Standard Method Performance Requirements (Appendix F). Official methods of analysis of AOAC International. AOAC International, Rockville, MD, USA. Available at:http://www.eoma.aoac.org/app_f.pdf
-
Atherstone, C., 2016. Assessing the impact of aflatoxin consumption on animal health and productivity. African Journal of Food, Agriculture, Nutrition and Development 16: 10949-10966.https://doi.org/10.18697/ajfand.75.ILRI01
-
Azri, F., Sukor, R., Selamat, J., Abu Bakar, F., Yusof, N. and Hajian, R., 2018. Electrochemical immunosensor for detection of aflatoxin B1 based on indirect competitive ELISA. Toxins 10: 196.https://doi.org/10.3390/toxins10050196
-
Calori-Domingues, M.A., Iwahashi, P.M.R., Ponce, G.H., Gloria, E.M., Dias, C.T.S., Button, D.C. and De Camargo, A.C., 2018. Aflatoxin B1 and zearalenone in soybeans: occurrence and distribution in whole and defective kernels. Food Additives and Contaminants Part B 11: 273-280.https://doi.org/10.1080/19393210.2018.1502818
-
Coppock, R.W., Christian, R.G. and Jacobsen B.J., 2018. Aflatoxins. In Gupta, R.C. (ed.) Veterinary toxicology 3. Elsevier, Amsterdam, the Netherlands, pp. 983-994.https://doi.org/10.1016/B978-0-12-811410-0.00069-6
-
Cotty, P.J. and Jaime-Garcia, R., 2007. Influences of climate on aflatoxin producing fungi and aflatoxin contamination. International Journal of Food Microbiology 119: 109-115.https://doi.org/10.1016/j.ijfoodmicro.2007.07.060
-
Deeb, A.M., Aman, I.M. and El-Hawary, I., 2016. Determination of aflatoxin M1 and B1 in Egyptian raw milk, soft cheese and table eggs using ELISA technique. International Journal of Innovative Research in Science and Engineering 4: 154-159.
'Determination of aflatoxin M1 and B1 in Egyptian raw milk, soft cheese and table eggs using ELISA technique ' () 4 International Journal of Innovative Research in Science and Engineering : 154 -159.
-
Eaton, D.L., Beima, K.M., Bammler, T.K., Riley, R.T. and Voss K.A., 2010. Hepatotoxic mycotoxins. Comprehensive Toxicology 2: 527-569.
'Hepatotoxic mycotoxins ' () 2 Comprehensive Toxicology : 527 -569.
-
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), 2004. Manual de Segurança e Qualidade para Avicultura de Postura, Projeto PAS Campo. Embrapa/sede, Brasília, Brazil. Available at:https://tinyurl.com/2p9as32v.
-
European Commission (EC), 2006. Commission Regulation No 401/2006 of 23 February 2006, laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Official Journal of the European Union L 70: 12-34.
-
Getachew, A., Chala, A., Hofgaard, I.S., Brurberg, M.B., Sulyok, M. and Tronsmo, A-M., 2017. Multimycotoxin and fungal analysis of maize grains from south and southwestern Ethiopia. Food Additives and Contaminants Part B 11: 64-74.https://doi.org/10.1080/19393210.2017.1408698
-
Gul, H., Khan, S., Shah, Z., Ahmad, S., Israr, M. and Hussain, M., 2016. Effects of local sodium bentonite as aflatoxin binder and its effects on production performance of laying hens. Kafkas Universitesi Veteriner Fakultesi Dergisi 23: 31-37.https://doi.org/10.9775/kvfd.2016.15714
-
Hajnal, E.J., Kos, J., Krulj, J., Krstović, S., Jajić, I., Pezo, L., Šarić, B. and Nedeljković, N., 2017. Aflatoxins contamination of maize in Serbia: the impact of weather conditions in 2015. Food Additives and Contaminants Part A 34: 1999-2010.https://doi.org/10.1080/19440049.2017.1331047.
-
Herzallah, S.M., 2009. Determination of aflatoxins in eggs, milk, meat and meat products using HPLC fluorescent and UV detectors. Food Chemistry 114: 1141-1146https://doi.org/10.1016/j.foodchem.2008.10.077
-
Insituto Agronômico do Paraná (IAPAR). 2021. Médias históricas para Londrina-PR. IAPAR, Londrina, Brazil. Available at:https://tinyurl.com/498kamy9.
-
Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), 2020. Orientação Sobre Validação de Métodos Analíticos. DOQCGCRE-008 Revisão 09. Ministério da Economia, Brasília, Brazil. Available at:https://tinyurl.com/zvn4uh2s
-
Kabak, B., Dobson, A.D.W. and Var, I., 2006. Strategies to prevent mycotoxin contamination of food and animal feed: a review. Critical Reviews in Food Science and Nutrition 46: 593-619.https://doi.org/10.1080/10408390500436185
-
Kawamura, O., Nagayama, S., Sato, S., Ohtani, K., Ueno, I. and Ueno, Y., 1988. A monoclonal antibody-based enzyme-linked immunosorbent assay of aflatoxin B1 in peanut products. Mycotoxin Research 4: 75-88.https://doi.org/10.1007/BF03192102
-
Kotinagu, K., Mohanamba, T. and Kumari, L.R., 2015. Assessment of aflatoxin B1 in livestock feed and feed ingredients by highperformance thin layer chromatography. Veterinary World 8: 1396-1399.https://doi.org/10.14202/vetworld.2015.1396-1399
-
Lee, M., Seo, D.J., Jeon, S.B., Ok, H.E., Jung, H., Choi, C. and Chun, H.S., 2016. Detection of foodborne pathogens and mycotoxins in eggs and chicken feeds from farms to retail markets. Korean Journal for Food Science of Animal Resources 36: 463-468.https://doi.org/10.5851/kosfa.2016.36.4.463
-
Lee, N.A., Wang, S., Allan, R.D. and Kennedy, I.R., 2004. A rapid aflatoxin B1 ELISA: development and validation with reduced matrix effects for peanuts, corn, pistachio, and soybeans. Journal of Agricultural and Food Chemistry 52: 2746-2755.https://doi.org/10.1021/jf0354038
-
Mgbeahuruike, A.C., Ejioffor, T.E., Christian, O.C., Shoyinka, V.C., Karlsson, M. and Nordkvist, E., 2018. Detoxification of aflatoxin-contaminated poultry feeds by 3 adsorbents, bentonite, activated charcoal, and Fuller’s earth. Journal of Applied Poultry Research 27: 461-471.https://doi.org/10.3382/japr/pfy054.
-
Monson, M., Coulombe, R. and Reed, K., 2015. Aflatoxicosis: lessons from toxicity and responses to aflatoxin B1 in poultry. Agriculture 5: 742-777.https://doi.org/10.3390/agriculture5030742
-
Morrison, D., Ledoux, D., Chester, L. and Samuels, C., 2017. A limited survey of aflatoxins in poultry feed and feed ingredients in Guyana. Veterinary Sciences 4: 60.https://doi.org/10.3390/vetsci4040060
-
Muga, F.C., Marenya, M.O. and Workneh, T.S., 2019. Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels. Bulgarian Journal of Agriculture Science 25: 271-277.
'Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels ' () 25 Bulgarian Journal of Agriculture Science : 271 -277.
-
Nomura, M., Aoyama, K. and Ishibashi, T., 2018. Sterigmatocystin and aflatoxin B1 contamination of corn, soybean meal, and formula feed in Japan. Mycotoxin Research 34: 21-27.https://doi.org/10.1007/s12550-017-0295-9
-
Oliveira, C.A.F., Kobashigawa, E., Reis, T.A., Mestieri, L., Albuquerque, R. and Correa, L.M.B., 2000. Aflatoxin B1 residues in eggs of laying hens fed a diet containing different levels of the mycotoxin. Food Additives and Contaminants 17: 459-462.https://doi.org/10.1080/02652030050034037
-
Oliveira, C.A.F., Rosmaninho, J.F., Castro, A.L., Butkeraitis, P., Reis, T.A. and Corrêa, B., 2003. Aflatoxin residues in eggs of laying Japanese quail after long-term administration of rations containing low levels of aflatoxin B1. Food Additives and Contaminants 20: 648-653.https://doi.org/10.1080/0265203031000119025
-
Oplatowska-Stachowiak, M., Sajic, N., Xu, Y., Haughey, S.A., Mooney, M.H., Gong, Y.Y. and Elliott, C.T., 2016. Fast and sensitive aflatoxin B1 and total aflatoxins ELISAs for analysis of peanuts, maize and feed ingredients. Food Control 63: 239-245.https://doi.org/10.1016/j.foodcont.2015.11.041
-
Rawal, S., Kim, J.E. and Coulombe, R., 2010. Aflatoxin B1 in poultry: toxicology, metabolism and prevention. Research in Veterinary Science 89: 325-331.https://doi.org/10.1016/j.rvsc.2010.04.011
-
Rossi, C.N., Takabayashi, C.R., Ono, M.A., Saito, G.H., Itano, E.N., Kawamura, O. and Ono, E.Y.S., 2012. Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed. Food Chemistry 132(4): 2211-2216.https://doi.org/10.1016/j.foodchem.2011.12.067
-
Saath, K.C.O. and Fachinello, A.L., 2018. Crescimento da demanda mundial de alimentos e restrições do fator terra no Brasil. Revista de Economia e Sociologia Rural 56: 195-212.https://doi.org/10.1590/1234-56781806-94790560201
-
Salhab, A.S. and Edwards, G.S., 1977. Comparative in vitro metabolism of aflatoxicol by liver preparations from Animals and Humans. Cancer Research 37: 1016-1021.
'Comparative in vitro metabolism of aflatoxicol by liver preparations from Animals and Humans ' () 37 Cancer Research : 1016 -1021.
-
Sireesha, Y., Keerthana, J.V., Sarathchandra, G. and Velazhahan, R., 2017. Analysis of aflatoxin B1 and aflatoxigenic mold in commercial poultry feeds in Tamil Nadu, India. Journal of Applied and Natural Science 9: 456-460.https://doi.org/10.31018/jans.v9i1.1213
-
Smith, J.W. and Groopman, J.D., 2019. Aflatoxins. In: Boffetta, P. and Hainaut, P. (eds.) Encyclopedia of Cancer (3rd ed.). Academic Press, Oxford, UK, pp. 30-43.
'Aflatoxins ', () 30 -43.
-
Trucksess, M.W., Stoloff, L., Young, K., Wyatt, R.D. and Miller, B.L., 1983. Aflatoxicol and aflatoxins B1 and M1 in eggs and tissues of laying hens consuming aflatoxin-contaminated feed. Poultry Science 62: 2176-2182.
'Aflatoxicol and aflatoxins B1 and M1 in eggs and tissues of laying hens consuming aflatoxin-contaminated feed ' () 62 Poultry Science : 2176 -2182.
-
Tulayakul, P., Mingkhwan, R., Hananantachai, H., Netvichian, R., Khaodhiar, S. and Songserm, T., 2018. Heavy metal (Cd and Pb) and aflatoxin contamination in tissues and eggs from free grazing ducks and their environment in central Thailand. Biological Trace Element Research 186: 514-520.https://doi.org/10.1007/s12011-018-1321-2
-
U.S. Food and Drug Administration (US FDA), 2011. Guidance for industry: action levels for poisonous or deleterious substances in human food and animal feed. US FDA, Silver Spring, MD, USA. Available at:https://tinyurl.com/yve7hakh.
-
Wacoo, A.P., Wendiro, D., Vuzi, P.C. and Hawumba, J.F., 2014. Methods for detection of aflatoxins in agricultural food crops. Journal of Applied Chemistry 1: 1-15.https://doi.org/10.1155/2014/706291
-
Zheng, Z., Humphrey, C.W., King, R.S. and Richard, J.L., 2005. Validation of an ELISA test kit for the detection of total aflatoxins in grain and grain products by comparison with HPLC. Mycopathologia 159: 255-263.https://doi.org/10.1007/s11046-004-8666-0
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Aflatoxin B1 in the egg chain: monitoring with specific indirect competitive ELISA in northern Paraná, Brazil
In: World Mycotoxin JournalSearch for other papers by F.G. Silva in
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Search for other papers by L.M.M. Zanin in
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An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.
| All Time | Past 365 days | Past 30 Days | |
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| Abstract Views | 228 | 184 | 27 |
| Full Text Views | 19 | 0 | 0 |
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