Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay
In: World Mycotoxin JournalSearch for other papers by J.P. Meneely in
Current site
Google Scholar
Search for other papers by J.G. Quinn in
Current site
Google Scholar
Search for other papers by E.M. Flood in
Current site
Google Scholar
Search for other papers by J. Hajšlová in
Current site
Google Scholar
Search for other papers by C.T. Elliott in
Current site
Google Scholar
This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibition format employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved an equal mixture of HT-2 and DON covalently coupled onto a high capacity COOH5 sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it is feasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 μg/kg for DON and 31, 47 and 36 μg/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respectively. Both intra-assay and inter-assay precision were calculated using fortified DON and HT-2 samples. Durum wheat, wheatbased breakfast cereal and maize-based baby food were spiked at various concentration levels and the coefficients of variation calculated ranged from 1.1% to 9.9% for DON and from 1.4% to 11.3% for HT-2. A high correlation was observed between the screening assay and confirmatory mass spectrometry.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Barna-Vetró, I., Gyöngyösi, A. and Solti, L., 1994. Monoclonal antibody-based enzyme-linked immunosorbent assay of Fusarium T-2 and zearalenone toxins in cereals. Applied and Environmental Microbiology 60: 729-731.
'Monoclonal antibody-based enzyme-linked immunosorbent assay of Fusarium T-2 and zearalenone toxins in cereals ' () 60 Applied and Environmental Microbiology : 729 -731 .
-
Biancardi, A., Gasparini, M., Dall’Astra, C. and Marchelli, R., 2005. A rapid multiresidual determination of type A and type B trichothecenes in wheat flour by HPLC-ESI-MS. Food Additives and Contaminants 22: 251-258.
'A rapid multiresidual determination of type A and type B trichothecenes in wheat flour by HPLC-ESI-MS ' () 22 Food Additives and Contaminants : 251 -258 .
-
Baumgartner, S., Führer, M. and Krska, R., 2010. Comparison of monoclonal antibody performance characteristics for the detection of two representatives of A- and B-trichothecenes: T-2 toxin and deoxynivalenol. World Mycotoxin Journal 3: 233-238.
'Comparison of monoclonal antibody performance characteristics for the detection of two representatives of A- and B-trichothecenes: T-2 toxin and deoxynivalenol ' () 3 World Mycotoxin Journal : 233 -238 .
-
Council for Agricultural Science and Technology (CAST), 2003. Mycotoxins: risks in plant, animal and human systems. Task Force Report No. 139, Ames, IA, USA.
-
De Saeger, S. and Van Peteghem, C., 1996. Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat. Applied and Environmental Microbiology 62: 1880-1884.
'Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat ' () 62 Applied and Environmental Microbiology : 1880 -1884 .
-
Dorokhin, D., Haasnoot, W., Franssen, M.C.R., Zuilhof, H. and Nielen, M.W.F., 2011. Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins. Analytical and Bioanalytical Chemistry 400: 3005-3011.
'Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins ' () 400 Analytical and Bioanalytical Chemistry : 3005 -3011 .
-
European Commission (EC), 2005. Council Regulation (EC) No 856/2005 of 6 June 2005 amending Regulation (EC) No 466/2001 as regards Fusarium toxins. Official Journal of the European Union L143: 3-8.
-
European Commission (EC), 2006a. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union L364: 5-24.
-
European Commission (EC), 2006b. Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Official Journal of the European Union L70: 12-34.
-
European Commission (EC), 2007. Commission Regulation (EC) No 1126/2007 of 28 September 2007 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Official Journal of the European Union L255: 14-17.
-
Edwards, S.G., Barrier-Guillot, B., Clasen, P-E., Hietaniemi, V. and Pettersson, H., 2009. Emerging issues of HT-2 and T-2 toxins in European cereal production. World Mycotoxin Journal 2: 173-179.
'Emerging issues of HT-2 and T-2 toxins in European cereal production ' () 2 World Mycotoxin Journal : 173 -179 .
-
Gendloff, E.H., Pestka, J.J., Swanson, P. and Hart, L.P., 1984. Detection of T-2 toxin in Fusarium sporotrichioides-infected corn by enzyme-linked immunosorbent assay. Applied and Environmental Microbiology 47: 1161-1163.
'Detection of T-2 toxin in Fusarium sporotrichioides-infected corn by enzyme-linked immunosorbent assay ' () 47 Applied and Environmental Microbiology : 1161 -1163 .
-
Gutleb, A.C., Morrison, E. and Murk, A.J., 2002. Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review. Environmental Toxicology and Pharmacology 11: 309-320.
'Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review ' () 11 Environmental Toxicology and Pharmacology : 309 -320 .
-
Jiménez, M., Mateo, J.J. and Mateo, R., 2000. Determination of type A trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection. Journal of Chromatography A 870: 473-481.
'Determination of type A trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection ' () 870 Journal of Chromatography A : 473 -481 .
-
Kadota, T., Takezawa, Y., Hirano, S., Tajima, O., Maragos, C.M., Nakajima, T., Tanaka, T., Kamata, Y. and Sugita-Konishi, Y., 2010. Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay. Analytica Chimica Acta 673: 173-178.
'Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay ' () 673 Analytica Chimica Acta : 173 -178 .
-
Kolosova, A.Y., Sibanda, L., Dumoulin, F., Lewis, J., Duveiller, E., Van Peteghem, C. and De Saeger, S., 2008. Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges. Analytica Chimica Acta 616: 235-244.
'Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges ' () 616 Analytica Chimica Acta : 235 -244 .
-
Lancova, K., Bowens, P., Stroka, J., Gmuender, H., Ellinger, T. and Naegeli, H., 2009. Transcriptomic-based bioassays for the detection of type A trichothecenes. World Mycotoxin Journal 2: 247-257.
'Transcriptomic-based bioassays for the detection of type A trichothecenes ' () 2 World Mycotoxin Journal : 247 -257 .
-
Lippolis, V., Pascale, M. and Visconti, A., 2006. Optimization of a fluorescence polarization immunoassay for rapid quantification of deoxynivalenol in durum wheat-based products. Journal of Food Protection 69: 2712-2719.
'Optimization of a fluorescence polarization immunoassay for rapid quantification of deoxynivalenol in durum wheat-based products ' () 69 Journal of Food Protection : 2712 -2719 .
-
Maragos, C.M. and McCormick, S.P., 2000. Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol. Food and Agricultural Immunology 12: 181-192.
'Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol ' () 12 Food and Agricultural Immunology : 181 -192 .
-
Maragos, M. and Plattner, R.D., 2002. Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat. Journal of Agricultural and Food Chemistry 50: 1827-1830.
'Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat ' () 50 Journal of Agricultural and Food Chemistry : 1827 -1830 .
-
Melchert, H.-U. and Pabel, E., 2004. Reliable identification and quantification of trichothecenes and other mycotoxins by electron impact and chemical ionization-gas chromatography-mass spectrometry, using ion-trap system in the multiple mass spectrometry mode, candidate reference method for complex matrices. Journal of Chromatography A 1056: 195-199.
'Reliable identification and quantification of trichothecenes and other mycotoxins by electron impact and chemical ionization-gas chromatography-mass spectrometry, using ion-trap system in the multiple mass spectrometry mode, candidate reference method for complex matrices ' () 1056 Journal of Chromatography A : 195 -199 .
-
Meneely, J.P., Fodey, T., Armstrong, L., Sulyok, M., Krska, R. and Elliott, C.T., 2010a. Rapid surface plasmon resonance immunoassay for the determination of deoxynivalenol in wheat, wheat products, and maize-based baby food. Journal of Agricultural and Food Chemistry 58: 8936-8941.
'Rapid surface plasmon resonance immunoassay for the determination of deoxynivalenol in wheat, wheat products, and maize-based baby food ' () 58 Journal of Agricultural and Food Chemistry : 8936 -8941 .
-
Meneely, J.P., Ricci, F., Van Egmond, H.P. and Elliott, C.T., 2011. Current methods of analysis for the determination of trichothecene mycotoxins in food. Trends in Analytical Chemistry 30: 192-203.
'Current methods of analysis for the determination of trichothecene mycotoxins in food ' () 30 Trends in Analytical Chemistry : 192 -203 .
-
Meneely, J.P., Sulyok, M., Baumgartner, S., Krska, R. and Elliott, C.T., 2010b. A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food. Talanta 81: 630-636.
'A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food ' () 81 Talanta : 630 -636 .
-
Molinelli, A., Grossalber, K., Führer, M., Baumgartner, S., Sulyok, M. and Krska, R., 2008. Development of qualitative and semiquantitative immunoassay-based rapid strip tests for the detection of T-2 toxin in wheat and oat. Journal of Agricultural and Food Chemistry 56: 2589-2594.
'Development of qualitative and semiquantitative immunoassay-based rapid strip tests for the detection of T-2 toxin in wheat and oat ' () 56 Journal of Agricultural and Food Chemistry : 2589 -2594 .
-
Pal, A., Acharya, D., Saha, D. and Dhar, T.K., 2004. Development of a membrane-based immunofiltration assay for the detection of T-2 toxin. Analytical Chemistry 76: 4237-4240.
'Development of a membrane-based immunofiltration assay for the detection of T-2 toxin ' () 76 Analytical Chemistry : 4237 -4240 .
-
Pascale, M., Haidukowski, M. and Visconti, A., 2003. Determination of T-2 toxin in cereal grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and derivatization with 1-anthroynitrile. Journal of Chromatography A 989: 257-264.
'Determination of T-2 toxin in cereal grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and derivatization with 1-anthroynitrile ' () 989 Journal of Chromatography A : 257 -264 .
-
Peters, J., Bienenmann-Ploum, M., De Rijk, T. and Haasnoot, W., 2011. Development of a multiplex cytometric microsphere immunoassay for mycotoxins and evaluation of its application in feed. Mycotoxin Research 27: 63-72.
'Development of a multiplex cytometric microsphere immunoassay for mycotoxins and evaluation of its application in feed ' () 27 Mycotoxin Research : 63 -72 .
-
Piermarini, S., Volpe, G., Ricci, F., Micheli, L., Moscone, D., Palleschi, G., Führer, M., Krska, R. and Baumgartner, S., 2007. Rapid screening electrochemical methods for aflatoxin B1 and type-A trichothecenes: a preliminary study. Analytical Letters 40: 1333-1346.
'Rapid screening electrochemical methods for aflatoxin B1 and type-A trichothecenes: a preliminary study ' () 40 Analytical Letters : 1333 -1346 .
-
Pussemier, L., Piérard, J.-Y., Anselme, M., Tangni, E. K., Motte, J.-C. and Larondelle, Y., 2006. Development and application of analytical methods for the determination of mycotoxins in organic and conventional wheat. Food Additives and Contaminants 23: 1208-1218.
'Development and application of analytical methods for the determination of mycotoxins in organic and conventional wheat ' () 23 Food Additives and Contaminants : 1208 -1218 .
-
Ricci, F., Abagnale, M., Messia, M.C., Marconi, E., Volpe, G., Moscone, D. and Palleschi, G., 2009. Direct electrochemical detection of trichothecenes in wheat samples using a 96-well electrochemical plate coupled with microwave hydrolysis. World Mycotoxin Journal 2: 239-245.
'Direct electrochemical detection of trichothecenes in wheat samples using a 96-well electrochemical plate coupled with microwave hydrolysis ' () 2 World Mycotoxin Journal : 239 -245 .
-
Romanazzo, D., Ricci, F., Volpe, G., Elliott, C.T., Vesco, S., Kroeger, K., Moscone, D., Stroka, J., Van Egmond, H., Vehniäinen, M. and Palleschi, G., 2010, Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples. Biosensors and Bioelectronics 25: 2615-2621.
'Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples ' () 25 Biosensors and Bioelectronics : 2615 -2621 .
-
Schneider, L., Pichler, H. and Krska, R., 2000, An enzyme linked immunoassay for the determination of deoxynivalenol in wheat based on chicken egg yolk antibodies. Fresenius Journal of Analytical Chemistry 367: 98-100.
'An enzyme linked immunoassay for the determination of deoxynivalenol in wheat based on chicken egg yolk antibodies ' () 367 Fresenius Journal of Analytical Chemistry : 98 -100 .
-
Schnerr, H., Vogel, R.F. and Niessen, L., 2002. A biosensor-based immunoassay for rapid screening of deoxynivalenol contamination in wheat. Food and Agricultural Immunology 14: 313-321.
'A biosensor-based immunoassay for rapid screening of deoxynivalenol contamination in wheat ' () 14 Food and Agricultural Immunology : 313 -321 .
-
Schothorst, R.C. and Jekel, A.A., 2001. Determination of trichothecenes in wheat by capillary gas chromatography with flame ionisation detection. Food Chemistry 73: 111-117.
'Determination of trichothecenes in wheat by capillary gas chromatography with flame ionisation detection ' () 73 Food Chemistry : 111 -117 .
-
Sokolović, M. and Şimpraga, B., 2006. Survey of trichothecene mycotoxins in grains and animal feed in Croatia by thin layer chromatography. Food Control 17: 733-740.
'Survey of trichothecene mycotoxins in grains and animal feed in Croatia by thin layer chromatography ' () 17 Food Control : 733 -740 .
-
Sulyok, M., Berthiller, F., Krska, R. and Schuhmacher, R., 2006. Development and validation of a liquid chromatography/tandem mass spectrometric method for the determination of 39 mycotoxins in wheat and maize. Rapid Communications in Mass Spectrometry 20: 2649-2659.
'Development and validation of a liquid chromatography/tandem mass spectrometric method for the determination of 39 mycotoxins in wheat and maize ' () 20 Rapid Communications in Mass Spectrometry : 2649 -2659 .
-
Tüdös, A.J., Lucas-Van Den Bos, E.R. and Stigter, E.C.A., 2003. Rapid surface plasmon resonance-based inhibition assay of deoxynivalenol. Journal of Agricultural and Food Chemistry 51: 5843-5848.
'Rapid surface plasmon resonance-based inhibition assay of deoxynivalenol ' () 51 Journal of Agricultural and Food Chemistry : 5843 -5848 .
-
Van der Gaag, B., Spath, S., Dietrich, H., Stigter, E., Boonzaaijer, G., Van Osenbruggen, T. and Koopal, K., 2003. Biosensors and multiple mycotoxins analysis. Food Control 14: 251-254.
'Biosensors and multiple mycotoxins analysis ' () 14 Food Control : 251 -254 .
-
Vendl, O., Berthiller, F., Crews, C. and Krska, R., 2009. Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC-MS-MS. Analytical and Bioanalytical Chemistry 395: 1347-1354.
'Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC-MS-MS ' () 395 Analytical and Bioanalytical Chemistry : 1347 -1354 .
-
Vishwanath, V., Sulyok, M., Labuda, R., Bicker, W. and Krska, R., 2009. Simultaneous determination of 186 fungal and bacterial metabolites in indoor matrices by liquid chromatography/tandem mass spectrometry. Analytical and Bioanalytical Chemistry 395: 1355-1372.
'Simultaneous determination of 186 fungal and bacterial metabolites in indoor matrices by liquid chromatography/tandem mass spectrometry ' () 395 Analytical and Bioanalytical Chemistry : 1355 -1372 .
-
Xiao, H., Clarke, J.R., Marquardt, R.R. and Frohlich, A.A., 1995. Improved methods for conjugating selected mycotoxins to carrier proteins and dextran for immunoassays. Journal of Agricultural and Food Chemistry 43: 2092-2097.
'Improved methods for conjugating selected mycotoxins to carrier proteins and dextran for immunoassays ' () 43 Journal of Agricultural and Food Chemistry : 2092 -2097 .
-
Yagen, B. and Joffe, Z., 1976. Screening of toxic isolates of Fusarium poae and Fusarium sporotrichioides involved in causing alimentary toxic aleukia. Applied and Environmental Microbiology 32: 423-427.
'Screening of toxic isolates of Fusarium poae and Fusarium sporotrichioides involved in causing alimentary toxic aleukia ' () 32 Applied and Environmental Microbiology : 423 -427 .
-
Yoshizawa, T., Kohno, H., Ikeda, K., Shinoda, T., Yokohama, H., Morita, K., Kusada, O. and Kobayashi, Y., 2004. A practical method for measuring deoxynivalenol, nivalenol and T-2 + HT-2 toxin in foods by an enzyme-linked immunosorbent assay using monoclonal antibodies. Bioscience, Biotechnology and Biochemistry 68: 2076-2085.
'A practical method for measuring deoxynivalenol, nivalenol and T-2 + HT-2 toxin in foods by an enzyme-linked immunosorbent assay using monoclonal antibodies ' () 68 Bioscience, Biotechnology and Biochemistry : 2076 -2085 .
-
Zachariasova, M., Lacina, O., Malachova, A., Kostelanska, M., Poustka, J., Godula, M. and Hajšlova, J., 2010. Novel approaches in the analysis of Fusarium mycotoxins in cereals employing ultra performance liquid chromatography coupled with high resolution mass spectrometry. Analytica Chimica Acta 662: 51-61.
'Novel approaches in the analysis of Fusarium mycotoxins in cereals employing ultra performance liquid chromatography coupled with high resolution mass spectrometry ' () 662 Analytica Chimica Acta : 51 -61 .
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 130 | 117 | 9 |
| Full Text Views | 19 | 2 | 0 |
| PDF Views & Downloads | 14 | 5 | 0 |
Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay
In: World Mycotoxin JournalSearch for other papers by J.P. Meneely in
Current site
Google Scholar
PubMed
Search for other papers by J.G. Quinn in
Current site
Google Scholar
PubMed
Search for other papers by E.M. Flood in
Current site
Google Scholar
PubMed
Search for other papers by J. Hajšlová in
Current site
Google Scholar
PubMed
Search for other papers by C.T. Elliott in
Current site
Google Scholar
PubMed
This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibition format employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved an equal mixture of HT-2 and DON covalently coupled onto a high capacity COOH5 sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it is feasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 μg/kg for DON and 31, 47 and 36 μg/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respectively. Both intra-assay and inter-assay precision were calculated using fortified DON and HT-2 samples. Durum wheat, wheatbased breakfast cereal and maize-based baby food were spiked at various concentration levels and the coefficients of variation calculated ranged from 1.1% to 9.9% for DON and from 1.4% to 11.3% for HT-2. A high correlation was observed between the screening assay and confirmatory mass spectrometry.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 130 | 117 | 9 |
| Full Text Views | 19 | 2 | 0 |
| PDF Views & Downloads | 14 | 5 | 0 |
