Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 assimilate cholesterol and modulate ABCA1, CD36, NPC1L1 and SCARB1 in vitro
In: Beneficial MicrobesCollaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by F.T. Lim in
Current site
Google Scholar
Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by S.M. Lim in
Current site
Google Scholar
Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by K. Ramasamy in
Current site
Google Scholar
There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively. For HT29 cells pre-treated with LAB, cholesterol lowering was accompanied by down-regulation of ATP-binding cassette family transporter-type A1 (ABCA1), cluster of differentiation 36 (CD36) and scavenger receptor class B member 1 (SCARB1). HT29 cells treated with LAB after prolonged exposure to cholesterol source, on the other hand, was associated with up-regulation of ABCA1, restoration of CD36 to basal level and down-regulation of Neimann-Pick C1-Like 1 (NPC1L1). The present findings implied the potential use of LAB4 and LAB12 as part of the strategies in prevention and management of hypercholesterolaemia.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Abumrad, N.A. and Davidson, N.O., 2012. Role of the gut in lipid homeostasis. Physiological Reviews 92: 1061-1085.
'Role of the gut in lipid homeostasis ' () 92 Physiological Reviews : 1061 -1085.
-
Anandharaj, M. and Sivasankari, B., 2014. Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property. Journal of Bioscience and Bioengineering 118: 153-159.
'Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property ' () 118 Journal of Bioscience and Bioengineering : 153 -159.
-
Bietrix, F., Yan, D., Nauze, M., Rolland, C., Bertrand-Michel, J., Comera, C., Schaak, S., Barbaras, R., Groen, A.K., Perret, B., Terce, F. and Collet, X., 2006. Accelerated lipid absorption in mice overexpressing intestinal SR-BI. Journal of Biological Chemistry 281: 7214-7219.
'Accelerated lipid absorption in mice overexpressing intestinal SR-BI ' () 281 Journal of Biological Chemistry : 7214 -7219.
-
Brunham, L.R., Kruit, J.K., Iqbal, J., Fievet, C., Timmins, J.M., Pape, T.D., Coburn, B.A., Bissada, N., Staels, B., Groen, A.K., Hussain, M.M., Parks, J.S., Kuipers, F. and Hayden, M.R., 2006. Intestinal ABCA1 directly contributes to HDL biogenesis in vivo. Journal of Clinical Investigation 116: 1052-1062.
'Intestinal ABCA1 directly contributes to HDL biogenesis in vivo ' () 116 Journal of Clinical Investigation : 1052 -1062.
-
Dai, F.J., Hsu, W.H., Huang, J.J. and Wu, S.C., 2013. Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters. Food and Chemical Toxicology 53: 384-391.
'Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters ' () 53 Food and Chemical Toxicology : 384 -391.
-
Davis Jr., H.R., Zhu, L.J., Hoos, L.M., Tetzloff, G., Maguire, M., Liu, J., Yao, X., Iyer, S.P., Lam, M.H., Lund, E.G. Detmers, P.A., Graziano, M.P. and Altmann, S.W., 2004. Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. Journal of Biological Chemistry 279: 33586-33592.
'Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis ' () 279 Journal of Biological Chemistry : 33586 -33592.
-
Duary, R.K., Rajput, Y.S., Batish, V.K. and Grover, S., 2011. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Indian Journal of Medical Research 134: 664-671.
'Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells ' () 134 Indian Journal of Medical Research : 664 -671.
-
During, A., Dawson, H.D. and Harrison, E.H., 2005. Carotenoid transport is decreased and expression of the lipid transporters SR-BI, NPC1L1, and ABCA1 is downregulated in Caco-2 cells treated with ezetimibe. Journal of Nutrition 135: 2305-2312.
'Carotenoid transport is decreased and expression of the lipid transporters SR-BI, NPC1L1, and ABCA1 is downregulated in Caco-2 cells treated with ezetimibe ' () 135 Journal of Nutrition : 2305 -2312.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: http://tinyurl.com/ou88oa2.
-
Field, F.J., Watt, K. and Mathur, S.N., 2008. Origins of intestinal ABCA1-mediated HDL-cholesterol. Journal of Lipid Research 49: 2605-2619.
'Origins of intestinal ABCA1-mediated HDL-cholesterol ' () 49 Journal of Lipid Research : 2605 -2619.
-
Gilliland, S.E., Nelson, C.R. and Maxwell, C., 1985. Assimilation of cholesterol by Lactobacillus acidophilus. Applied and Environmental Microbiology 49: 377-381.
'Assimilation of cholesterol by Lactobacillus acidophilus ' () 49 Applied and Environmental Microbiology : 377 -381.
-
Guo, C.F., Zhang, L.W., Han, X., Yi, H.X., Li, J.Y., Tuo, Y.F., Zhang, Y.C., DU, M., Shan, Y.J. and Yang, L., 2012. Screening for cholesterollowering probiotic based on deoxycholic acid removal pathway and studying its functional mechanisms in vitro. Anaerobe 18: 7.
'Screening for cholesterollowering probiotic based on deoxycholic acid removal pathway and studying its functional mechanisms in vitro ' () 18 Anaerobe : 7.
-
Huang, Y. and Zheng, Y., 2010. The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells. British Journal of Nutrition 103: 6.
'The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells ' () 103 British Journal of Nutrition : 6.
-
Hynonen, U. and Palva, A., 2013. Lactobacillus surface layer proteins: structure, function and applications. Applied Microbiology Biotechnology 97: 5225-5243.
'Lactobacillus surface layer proteins: structure, function and applications ' () 97 Applied Microbiology Biotechnology : 5225 -5243.
-
Iqbal, J. and Hussain, M.M., 2009. Intestinal lipid absorption. American Journal of Physiology: Endocrinology and Metabolism 296: E1183-1194.
'Intestinal lipid absorption ' () 296 American Journal of Physiology: Endocrinology and Metabolism : E1183 -1194.
-
Ishimwe, N., Daliri, E.B., Lee, B.H., Fang, F. and Du, G., 2015. The perspective on cholesterol-lowering mechanisms of probiotics. Molecular Nutrition and Food Research 59: 94-105.
'The perspective on cholesterol-lowering mechanisms of probiotics ' () 59 Molecular Nutrition and Food Research : 94 -105.
-
Jia, L., Betters, J.L. and Yu, L., 2011. Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport. Annual Review of Physiology 73: 239-259.
'Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport ' () 73 Annual Review of Physiology : 239 -259.
-
Kertesz, A., Bombicz, M., Priksz, D., Balla, J., Balla, G., Gesztelyi, R., Varga, B., Haines, D.D., Tosaki, A. and Juhasz, B., 2013. Adverse impact of diet-induced hypercholesterolemia on cardiovascular tissue homeostasis in a rabbit model: time-dependent changes in cardiac parameters. International Journal of Molecular Sciences 14: 19086-19108.
'Adverse impact of diet-induced hypercholesterolemia on cardiovascular tissue homeostasis in a rabbit model: time-dependent changes in cardiac parameters ' () 14 International Journal of Molecular Sciences : 19086 -19108.
-
Kumar, A., Kumar, M., Ghosh, M. and Ganguli, A., 2013. Modeling in vitro cholesterol reduction in relation to growth of probiotic Labtobacillus casei. Microbiology and Immunology 57: 12.
'Modeling in vitro cholesterol reduction in relation to growth of probiotic Labtobacillus casei ' () 57 Microbiology and Immunology : 12.
-
Liu, R., Li, J., Zhang, T., Zou, L., Chen, Y., Wang, K., Lei, Y., Yuan, K., Li, Y., Lan, J., Cheng, L., Xie, N., Xiang, R., Nice, E.C., Huang, C. and Wei, Y., 2014. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10: 1241-1255.
'Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking ' () 10 Autophagy : 1241 -1255.
-
Lye, H.S., Rahmat-Ali, G.R. and Liong, M.T., 2010a. Mechanisms of cholesterol removal by lactobacilli under conditions that mimic the human gastrointestinal tract. International Dairy Journal 20: 7.
'Mechanisms of cholesterol removal by lactobacilli under conditions that mimic the human gastrointestinal tract ' () 20 International Dairy Journal : 7.
-
Lye, H.S., Rusul, G. and Liong, M.T., 2010b. Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol. Journal of Dairy Science 93: 11.
'Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol ' () 93 Journal of Dairy Science : 11.
-
Malmendier, C.L. and Alaupovic, P., 2012. Lipoproteins and Atherosclerosis. Springer, New York, NY, USA.
'Lipoproteins and Atherosclerosis', ().
-
Mardones, P., Quinones, V., Amigo, L., Moreno, M., Miquel, J.F., Schwarz, M., Miettinen, H.E., Trigatti, B., Krieger, M., VanPatten, S., Cohen, D.E. and Rigotti, A., 2001. Hepatic cholesterol and bile acid metabolism and intestinal cholesterol absorption in scavenger receptor class B type I-deficient mice. Journal of Lipid Research 42: 170-180.
'Hepatic cholesterol and bile acid metabolism and intestinal cholesterol absorption in scavenger receptor class B type I-deficient mice ' () 42 Journal of Lipid Research : 170 -180.
-
McNeish, J., Aiello, R.J., Guyot, D., Turi, T., Gabel, C., Aldinger, C., Hoppe, K.L., Roach, M.L., Royer, L.J., De Wet, J., Broccardo, C., Chimini, G. and Francone, O.L., 2000. High density lipoprotein deficiency and foam cell accumulation in mice with targeted disruption of ATP-binding cassette transporter-1. Proceedings of the National Academy of Sciences 97: 4245-4250.
'High density lipoprotein deficiency and foam cell accumulation in mice with targeted disruption of ATP-binding cassette transporter-1 ' () 97 Proceedings of the National Academy of Sciences : 4245 -4250.
-
Michael, D.R., Moss, J.W.E., Lama Calvente, D., Garaiova, I., Plummer, S.F. and Ramji, D.P., 2016. Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes. Beneficial Microbes 7: 443-451.
'Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes ' () 7 Beneficial Microbes : 443 -451.
-
Miremadi, F., Ayyash, M., Sherkat, F. and Stojanovska, L., 2014. Cholesterol reduction mechanism and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria. Journal of Functional Foods 9: 295-305.
'Cholesterol reduction mechanism and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria ' () 9 Journal of Functional Foods : 295 -305.
-
Navarese, E.P., Szczesniak, A., Kolodziejczak, M., Gorny, B., Kubica, J. and Suryapranata, H., 2014. Statins and risk of new-onset diabetes mellitus: is there a rationale for individualized statin therapy? American Journal of Cardiovascular Drugs 14: 79-87.
'Statins and risk of new-onset diabetes mellitus: is there a rationale for individualized statin therapy? ' () 14 American Journal of Cardiovascular Drugs : 79 -87.
-
Ooi, L.G. and Liong, M.T., 2010. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. International Journal of Molecular Sciences 11: 24.
'Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings ' () 11 International Journal of Molecular Sciences : 24.
-
Oram, J.F. and Lawn, R.M., 2001. ABCA1: the gatekeeper for eliminating excess tissue cholesterol. Journal of Lipid Research 42: 7.
'ABCA1: the gatekeeper for eliminating excess tissue cholesterol ' () 42 Journal of Lipid Research : 7.
-
Ramasamy, K., Abdul-Rahman, N.Z., Sieo, C.C., Alitheen, N.J., Abdullah, N. and Ho, Y.W., 2012. Probiotic potential of lactic acid bacteria from fermented Malaysian food or milk products. International Journal of Food Sciences and Technologies 47: 9.
'Probiotic potential of lactic acid bacteria from fermented Malaysian food or milk products ' () 47 International Journal of Food Sciences and Technologies : 9.
-
Ramasamy, K., Abdullah, N., Wong, M.C., Karuthan, C. and Ho, Y.W., 2010. Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. Journal of the Science of Food and Agriculture 90: 65-69.
'Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens ' () 90 Journal of the Science of Food and Agriculture : 65 -69.
-
Rombeau, J.L. and Takala, J., 1996. Gut dysfunction in critical illness. Springer, New York, NY, USA.
'Gut dysfunction in critical illness', ().
-
Rudolf, M. and Curcio, C.A., 2009. Esterified cholesterol is highly localized to Bruch’s membrane, as revealed by lipid histochemistry in wholemounts of human choroid. Journal of Histochemistry and Cytochemistry 57: 731-739.
'Esterified cholesterol is highly localized to Bruch’s membrane, as revealed by lipid histochemistry in wholemounts of human choroid ' () 57 Journal of Histochemistry and Cytochemistry : 731 -739.
-
Tiwari, A., Bansal, V., Chugh, A. and Mookhtiar, K., 2006. Statins and myotoxicity: a therapeutic limitation. Expert Opinion on Drug Safety 5: 651-666.
'Statins and myotoxicity: a therapeutic limitation ' () 5 Expert Opinion on Drug Safety : 651 -666.
-
Tok, E. and Aslim, B., 2010. Cholesterol removal by some lactic acid bacteria that can be used as probiotic. Microbiology and Immunology 54: 257-264.
'Cholesterol removal by some lactic acid bacteria that can be used as probiotic ' () 54 Microbiology and Immunology : 257 -264.
-
Tomaro-Duchesneau, C., Jones, M.L., Shah, D., Jain, P., Saha, S. and Prakash, S., 2014. Cholesterol assimilation by Lactobacillus probiotic bacteria: an in vitro investigation. Biomedical Research International 2014: 380316.
'Cholesterol assimilation by Lactobacillus probiotic bacteria: an in vitro investigation ' () 2014 Biomedical Research International : 380316.
-
Tsai, C.C., Lin, P.P., Hsieh, Y.M., Zhang, Z.Y., Wu, H.C. and Huang, C.C., 2014. Cholesterol-lowering potentials of lactic acid bacteria based on bile-salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo. Scientific World Journal 2014: 690752.
'Cholesterol-lowering potentials of lactic acid bacteria based on bile-salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo ' () 2014 Scientific World Journal : 690752.
-
Wang, N., Silver, D.L., Thiele, C. and Tall, A.R., 2001. ATP-binding cassette transporter A1 (ABCA1) functions as a cholesterol efflux regulatory protein. Journal of Biological Chemistry 276: 23742-23747.
'ATP-binding cassette transporter A1 (ABCA1) functions as a cholesterol efflux regulatory protein ' () 276 Journal of Biological Chemistry : 23742 -23747.
-
Werder, M., Han, C.H., Wehrli, E., Bimmler, D., Schulthess, G. and Hauser, H., 2001. Role of scavenger receptors SR-BI and CD36 in selective sterol uptake in the small intestine. Biochemistry 40: 11643-11650.
'Role of scavenger receptors SR-BI and CD36 in selective sterol uptake in the small intestine ' () 40 Biochemistry : 11643 -11650.
-
Wu, C.C., Weng, W.L., Lai, W.L., Tsai, H.P., Liu, W.H., Lee, M.H. and Tsai, Y.C., 2015. Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice. Evidence-based Complementary and Alternative Medicine 2015: 391767.
'Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice ' () 2015 Evidence-based Complementary and Alternative Medicine : 391767.
-
Yoon, H.S., Ju, J.H., Kim, H., Lee, J., Park, H.J., Ji, Y., Shin, H.K., Do, M.S., Lee, J. and Holzapfel, W., 2011. Lactobacillus rhamnosus BFE 5264 and Lactobacillus plantarum NR74 promote cholesterol excretion through the up-regulation of ABCG5/8 in Caco-2 cells. Probiotics and Antimicrobial Proteins 3: 10.
'Lactobacillus rhamnosus BFE 5264 and Lactobacillus plantarum NR74 promote cholesterol excretion through the up-regulation of ABCG5/8 in Caco-2 cells ' () 3 Probiotics and Antimicrobial Proteins : 10.
-
Yoon, H.S., Ju, J.H., Kim, H.N., Park, H.J., Ji, Y., Lee, J.E., Shin, H.K., Do, M.S. and Holzapfel, W., 2013a. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus plantarum NR74 from fermented foods. International Journal of Food Sciences and Nutrition 64: 9.
'Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus plantarum NR74 from fermented foods. ' () 64 International Journal of Food Sciences and Nutrition : 9.
-
Yoon, H.S., Ju, J.H., Lee, J.E., Park, H.J., Lee, J.M., Shin, H.K., Holzapfel, W., Park, K.Y. and Do, M.S., 2013b. The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 cells. Journal of the Science of Food and Agriculture 93: 781-787.
'The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 cells. ' () 93 Journal of the Science of Food and Agriculture : 781 -787.
-
Yue, P., Chen, Z., Nassir, F., Bernal-Mizrachi, C., Finck, B., Azhar, S. and Abumrad, N.A., 2010. Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice. PLoS One 5: e9906.
'Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice ' () 5 PLoS One : e9906.
Supplementary Materials
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 155 | 148 | 19 |
| Full Text Views | 8 | 0 | 0 |
| PDF Views & Downloads | 7 | 1 | 0 |
Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 assimilate cholesterol and modulate ABCA1, CD36, NPC1L1 and SCARB1 in vitro
In: Beneficial MicrobesCollaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by F.T. Lim in
Current site
Google Scholar
PubMed
Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by S.M. Lim in
Current site
Google Scholar
PubMed
Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.
Search for other papers by K. Ramasamy in
Current site
Google Scholar
PubMed
There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively. For HT29 cells pre-treated with LAB, cholesterol lowering was accompanied by down-regulation of ATP-binding cassette family transporter-type A1 (ABCA1), cluster of differentiation 36 (CD36) and scavenger receptor class B member 1 (SCARB1). HT29 cells treated with LAB after prolonged exposure to cholesterol source, on the other hand, was associated with up-regulation of ABCA1, restoration of CD36 to basal level and down-regulation of Neimann-Pick C1-Like 1 (NPC1L1). The present findings implied the potential use of LAB4 and LAB12 as part of the strategies in prevention and management of hypercholesterolaemia.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 155 | 148 | 19 |
| Full Text Views | 8 | 0 | 0 |
| PDF Views & Downloads | 7 | 1 | 0 |