Obesity is highly correlated with the dysbiosis of intestinal microbiota, and bifidobacteria are one of the soft targets of this metabolic syndrome. The aim of this study is to evaluate the efficacy of Bifidobacterium sp. MKK4 and rice-based fermented foods on physical, haematological, gut microbiota and lypogenic-lypolytic marker genes in diet-induced obese mice. Adult male mice (21±0.7 g) were randomly divided into four groups (n=10) according to the type of diet: normal diet (ND), high fat diet (HFD), HFD supplemented with Bifidobacterium sp. MKK4 and HFD supplemented with MKK4 associated rice-fermented food. 8 weeks of bacterial therapy in the obese mice resulted in significant reduction of body and organ weights, improved serum levels of glucose, triglyceride and cholesterol, the histological structure of the liver (steatosis), and re-establishment of gut Lactobacillus, Bifidobacterium and Bacteroides species. The bacterial therapy led to up-regulation of lipolytic transcription factors, such as peroxisome proliferator-activated receptor (PPAR)-α, PPAR-δ, and their regulated gene products in fatty acid metabolism and glucose uptake, such as acyl-CoA oxidase, carnitine palmitoyl-transferase-1, uncoupling protein-3 and glucose transporter-4. Concomitantly, both adipocytogenesis and fatty acid synthesis were arrested as reflected by the down-regulation of sterol regulatory element binding protein-1c, acetyl-CoA carboxylase, fatty acid synthase and tumour necrosis factor alpha genes. The effectiveness of the fermented product was more profound than the single bacterium. These data provide experimental support with regard to the use of Bifidobacterium sp. MKK4 as a natural therapeutic agent to control obesity.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Alard, J., Lehrter, V., Rhimi, M., Manqin, I., Peucelle, V., Abraham, A.L., Mariadassou, M., Maquin, E., Waliqora-Dupriet, A.J., Pot, B., Wolowczuk, I. and Granqrtte, C., 2016. Beneficial metabolic effects of selected probiotics on diet-induced obesity and insulin resistance in mice are associated with improvement of dysbiotic gut microbiota. Environmental Microbiology 18: 1484-1497.
'Beneficial metabolic effects of selected probiotics on diet-induced obesity and insulin resistance in mice are associated with improvement of dysbiotic gut microbiota ' () 18 Environmental Microbiology : 1484 -1497.
Bindels, L.B., Dewulf, E.M. and Delzenne, N.M., 2013. GPR43/FFA2: physiopathological relevance and therapeutic prospects. Trends in Pharmacological Sciences 34: 226-232.
'GPR43/FFA2: physiopathological relevance and therapeutic prospects ' () 34 Trends in Pharmacological Sciences : 226 -232.
Bray, G.A. and Tartaglia, L.A., 2000. Medicinal strategies in the treatment of obesity. Nature 404: 672-677.
'Medicinal strategies in the treatment of obesity ' () 404 Nature : 672 -677.
Cani, P.D., Neyrinck, A.M., Fava, F., Knauf, C., Buecelin, R.G., Tuohy, K.M., Gibson, G.R. and Deizenne, N.M., 2007. Selective increases of bifidobacteria in gut microflora improves high-fat diet induced diabetes in mice through a mechanism associated with endotoxemia. Diabetologia 50: 374-383.
'Selective increases of bifidobacteria in gut microflora improves high-fat diet induced diabetes in mice through a mechanism associated with endotoxemia ' () 50 Diabetologia : 374 -383.
Cani, P.D., Possemiers, S., Van de Wiele, T., Guiot, Y., Everard, A., Rottier, O., Geurts, L., Naslain, D., Neyrinck, A., Lambert, D.M., Muccioli, G.G. and Delzenne, N.M., 2009. Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 58: 1091-1103.
'Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability ' () 58 Gut : 1091 -1103.
Chen, J.J., Wang, R., Li, X.F. and Wang, R.L., 2011. Bifidobacterium longum supplementation improved high-fat-fed-induced metabolic syndrome and promoted intestinal Reg I gene expression. Experimental Biology and Medicine 236: 823-831.
'Bifidobacterium longum supplementation improved high-fat-fed-induced metabolic syndrome and promoted intestinal Reg I gene expression ' () 236 Experimental Biology and Medicine : 823 -831.
Delzenne, N.M. and Cani, P.D., 2011. Interaction between obesity and the gut microbiota: relevance in nutrition. Annual Review of Nutrition 31: 15-31.
'Interaction between obesity and the gut microbiota: relevance in nutrition ' () 31 Annual Review of Nutrition : 15 -31.
Du Toit, M., Franz, C.M., Dicks, L.M., Schillinger, U., Haberer, P., Warlies, B., Arhens, F. and Holzapfel, W.H., 1998. Characterization and selection of probiotic lactobacilli for a preliminary mini pig feeding trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content. International Journal of Food Microbiology 40: 93-104.
'Characterization and selection of probiotic lactobacilli for a preliminary mini pig feeding trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content ' () 40 International Journal of Food Microbiology : 93 -104.
Esposito, E., Iacono, A., Bianco, G., Autore, G., Cuzzocrea, S., Vajro, P., Canani, R.B., Caliqnano, A., Raso, G.M. and Meli, R., 2009. Probiotics reduce the inflammatory response induced by a high-fat diet in the liver of young rats. Journal of Nutrition 139: 905-911.
'Probiotics reduce the inflammatory response induced by a high-fat diet in the liver of young rats ' () 139 Journal of Nutrition : 905 -911.
Gueimonde, M. and Sánchez, B., 2012. Enhancing probiotic stability in industrial processes. Microbial Ecology in Health and Disease 23: 18562.
'Enhancing probiotic stability in industrial processes ' () 23 Microbial Ecology in Health and Disease : 18562.
Hamad, E.M., Sato, M., Uzu, K., Yoshida, T., Higashi, S., Kawakami, H., Kadooka, Y., Matsuyama, H., Abd El-Gawad, I.A. and Imaizumi, K., 2009. Milk fermented by Lactobacillus gasseri SBT 2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats. British Journal of Nutrition 101: 716-724.
'Milk fermented by Lactobacillus gasseri SBT 2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats ' () 101 British Journal of Nutrition : 716 -724.
Kalliomaki, M., Collado, M.C., Salminen, S. and Isolauri, E., 2008. Early differences in fecal microbiota composition in children may predict overweight. American Journal of Clinical Nutrition 87: 534-538.
'Early differences in fecal microbiota composition in children may predict overweight ' () 87 American Journal of Clinical Nutrition : 534 -538.
Kang, J.-H., Yun, S.-I., Park, M.-H., Park, J.-H., Jeong, S.-Y. and Park, H.-O., 2013. Anti-obesity effect of Lactobacillus gasseri BNR17 in high-sucrose diet-induced obese mice. PloS One 8: e54617.
'Anti-obesity effect of Lactobacillus gasseri BNR17 in high-sucrose diet-induced obese mice ' () 8 PloS One : e54617.
Kimura, I., Inoue, D., Hirano, K. and Tsujimoto, G., 2014. The SCFA receptor GPR43 and energy metabolism. Frontiers in Endocrinology 5: 85.
'The SCFA receptor GPR43 and energy metabolism ' () 5 Frontiers in Endocrinology : 85.
Kobyliak, N., Conte, C., Cammarota, G., Haley, A.P., Styriak, I., Gaspar, L., Fusek, J., Rodrigo, L. and Kruzliak, P., 2016. Probiotics in prevention and treatment of obesity: a critical view. Nutrition and Metabolism 13: 14-27.
'Probiotics in prevention and treatment of obesity: a critical view ' () 13 Nutrition and Metabolism : 14 -27.
Kondo, S., Xiao, J.Z., Satoh, T., Satoh, T., Odamaki, T., Takahashi, S., Suqahara, H., Yaeshima, T., Iwatsuki, K., Kamei, A. and Abe, K., 2010. Antiobesity effects of Bifidobacterium breve strain B-3 supplementation in a mouse model with high-fat diet induced obesity. Bioscience, Biotechnology, and Biochemistry 74: 1656-1666.
'Antiobesity effects of Bifidobacterium breve strain B-3 supplementation in a mouse model with high-fat diet induced obesity ' () 74 Bioscience, Biotechnology, and Biochemistry : 1656 -1666.
Korner, J. and Aronne, L.J., 2004. Pharmacological approaches to weight reduction: therapeutic targets. Journal of Clinical Endocrinology and Metabolism 89: 2616-2621.
'Pharmacological approaches to weight reduction: therapeutic targets ' () 89 Journal of Clinical Endocrinology and Metabolism : 2616 -2621.
Le Poul, E., Loison, C., Struyf, S., Springael, J.Y., Lannay, V., Decobecq, M.E., Brezillon, S., Dupriez, V., Vassart, G., Van Damme, J., Paementier, M. and Detheux, M., 2003. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. Journal of Biological Chemistry 278: 25481-25489.
'Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation ' () 278 Journal of Biological Chemistry : 25481 -25489.
Lim, H.J., Kim, S.Y. and Lee, W.K., 2004. Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use. Journal of Veterinary Sciences 5: 391-395.
'Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use ' () 5 Journal of Veterinary Sciences : 391 -395.
Million, M., Maraninchi, M., Henry, M., Armouqom, F., Richet, H., Carrieri, P., Valero, R., Raccah, D., Vialettes, B. and Raoult, D., 2012. Obesity associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii. International Journal of Obesity 36: 817-825.
'Obesity associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii ' () 36 International Journal of Obesity : 817 -825.
Miyamoto, J., Hasegawa, S., Kasubuchi, M., Ichimura, A., Nakajima, A. and Kimura, I., 2016. Nutritional signalling via free fatty acid receptors. International Journal of Molecular Sciences 17: 450-462.
'Nutritional signalling via free fatty acid receptors ' () 17 International Journal of Molecular Sciences : 450 -462.
Mohan Reddy, N., Kalyana, K.C. and Jamil, K., 2012. New World Syndrome (obesity) in South India. Scientific Reports 1: 567.
'New World Syndrome (obesity) in South India ' () 1 Scientific Reports : 567.
Mondal, K.C., Ghosh, K., Mitra, B., Parua, S. and Das Mohapatra, P.K., 2016. Rice-based fermented foods and beverages: functional and nutraceutical properties. In: Ramesh, C., Ray, R.C. and Montet, D. (eds.) Fermented foods, part II: technological intervention. CRC Press, New York, NY, USA, pp. 150-176.
'Rice-based fermented foods and beverages: functional and nutraceutical properties ', () 150 -176.
National Institute of Health (NIH), 1998. Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults – the evidence report. National Hearth Lung and Blood Institute, Bethesda, MD, USA. Available at: http://tinyurl.com/y8eo47lp.
Nishida, C. and Mucavele, P., 2005. Monitoring the rapidly emerging public health problem of overweight and obesity: the WHO Global Database on Body Mass Index. SCN News 129: 5-12.
'Monitoring the rapidly emerging public health problem of overweight and obesity: the WHO Global Database on Body Mass Index ' () 129 SCN News : 5 -12.
Nova, E., De Heredia, F.P., Gómez-Martínez, S. and Marcos, A., 2016. The role of probiotics on the microbiota: effect on obesity. Nutrition in Clinical Practice 31: 387-400.
'The role of probiotics on the microbiota: effect on obesity ' () 31 Nutrition in Clinical Practice : 387 -400.
Ooi, L.G. and Liong, M.T., 2010. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. International Journal of Molecular Sciences 11: 2499-2522.
'Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings ' () 11 International Journal of Molecular Sciences : 2499 -2522.
Patel, A.K., Singhania, R.R., Pandey, A. and Chincholkar, S.B., 2010. Probiotic bile salt hydrolase: current developments and perspectives. Applied Biochemistry and Biotechnology 162: 166-180.
'Probiotic bile salt hydrolase: current developments and perspectives ' () 162 Applied Biochemistry and Biotechnology : 166 -180.
Pradeepa, R., Anjana, R.M., Joshi, S.R., Bhansali, A., Deepa, M., Joshi, P.P., Dhandania, V.K., Madhu, S.V., Rao, P.V., Geetha, L., Subashini, R., Unnikrishnan, R., Shukla, D.K., Kaur, T., Mohan, V., Das, A.K., 2015. Prevalence of generalized and abdominal obesity in urban and rural India – the ICMR-INDIAB study (Phase-I) [ICMR-INDIAB-3]. Indian Journal of Medical Research 142: 139-150.
'Prevalence of generalized and abdominal obesity in urban and rural India – the ICMR-INDIAB study (Phase-I) [ICMR-INDIAB-3] ' () 142 Indian Journal of Medical Research : 139 -150.
Ray, M., Ghosh, K., Har, P.K., Singh, S.N. and Mondal, K.C., 2017a. Fortification of rice gruel into functional beverage and establishment as a carrier of newly isolated Bifidobacterium sp. MKK4. Research Journal of Microbiology 12: 102-117.
'Fortification of rice gruel into functional beverage and establishment as a carrier of newly isolated Bifidobacterium sp ' () 12 Research Journal of Microbiology : 102 -117.
Ray, M., Ghosh, K., Har, P.K., Singh, S.N. and Mondal, K.C., 2017b. Screening of health beneficial microbes with potential probiotic characteristics from the traditional rice based alcoholic beverage, haria. Acta Biologica Szegediensis 61: 51-58.
'Screening of health beneficial microbes with potential probiotic characteristics from the traditional rice based alcoholic beverage, haria ' () 61 Acta Biologica Szegediensis : 51 -58.
Seo, J.B., Choe, S.S., Jeong, H.W., Park, S.W., Shin, H.J., Choi, S.M., Park, J.Y., Choi, E.W., Kim, J.B., Seen, D.S., Jeong, J.Y. and Lee, T.G., 2011. Anti-obesity effects of Lysimachia foenum-graecum characterized by decreased adipogenesis and regulated lipid metabolism. Experimental and Molecular Medicine 43: 205-215.
'Anti-obesity effects of Lysimachia foenum-graecum characterized by decreased adipogenesis and regulated lipid metabolism ' () 43 Experimental and Molecular Medicine : 205 -215.
Shori, A.B., 2016. Influence of food matrix on the viability of probiotic bacteria: a review based on dairy and non-dairy beverages. Food Bioscience 13: 1-8.
'Influence of food matrix on the viability of probiotic bacteria: a review based on dairy and non-dairy beverages ' () 13 Food Bioscience : 1 -8.
Strychar, I., 2006. Diet in the management of weight loss. Canadian Medical Association Journal 3: 56-63.
'Diet in the management of weight loss ' () 3 Canadian Medical Association Journal : 56 -63.
Tan, S., Gao, B., Tao, Y., Guo, J. and Su, Z., 2014. Antiobese effects of capsaicin-chitosan microsphere (CCMS) in obese rats induced by high fat diet. Journal of Agricultural and Food Chemistry 62: 1866-1874.
'Antiobese effects of capsaicin-chitosan microsphere (CCMS) in obese rats induced by high fat diet ' () 62 Journal of Agricultural and Food Chemistry : 1866 -1874.
Tarantino, G., 2014. Gut microbiome, obesity-related comorbidities, and low-grade chronic inflammation. Journal of Clinical Endocrinology and Metabolism 99: 2343-2346.
'Gut microbiome, obesity-related comorbidities, and low-grade chronic inflammation ' () 99 Journal of Clinical Endocrinology and Metabolism : 2343 -2346.
World Health Organization (WHO), 2000. Obesity: preventing and managing the global epidemic. Report of a WHO consultation on obesity. World Health Organ Tech Report Series 894: i-xii, 1-253.
'Obesity: preventing and managing the global epidemic ' () 894 World Health Organ Tech Report Series : 1 -253.
Wronska, A. and Kmiec, Z., 2012. Structural and biochemical characteristics of various white adipose tissue depots. Acta Physiologica 205: 194-208.
'Structural and biochemical characteristics of various white adipose tissue depots ' () 205 Acta Physiologica : 194 -208.
Xiao, J.Z., Kondo, S., Takahashi, N., Miyaji, K., Oshida, K., Hiramatsu, A., Iwatsuki, K., Kokubo, S. and Hosono, A., 2003. Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. Journal of Dairy Science 86: 2452-2461.
'Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers ' () 86 Journal of Dairy Science : 2452 -2461.
Yadav, H., Lee, J.H., Lloyd, J., Walter, P. and Rane, S.G., 2013. Beneficial metabolic effects of a probiotic via butyrate induced GLP-1 secretion. Journal of Biological Chemistry 288: 25088-25097.
'Beneficial metabolic effects of a probiotic via butyrate induced GLP-1 secretion ' () 288 Journal of Biological Chemistry : 25088 -25097.
Yin, Y.N., Yu, Q.F., Fu, N., Liu, X.W. and Lu, F.G., 2010. Effects of four bifidobacteria on obesity in high-fat diet induced rats. World Journal of Gastroenterology 16: 3394-3401.
'Effects of four bifidobacteria on obesity in high-fat diet induced rats ' () 16 World Journal of Gastroenterology : 3394 -3401.
Zeng, H., Liu, J., Jackson, M.I., Zhao, F.Q., Yan, L. and Combs Jr., G.F., 2013. Fatty liver accompanies an increase in Lactobacillus species in the hind gut of C57BL/6 mice fed a high-fat diet. Journal of Nutrition 143: 627-631.
'Fatty liver accompanies an increase in Lactobacillus species in the hind gut of C57BL/6 mice fed a high-fat diet ' () 143 Journal of Nutrition : 627 -631.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 185 | 172 | 25 |
Full Text Views | 12 | 6 | 0 |
PDF Views & Downloads | 10 | 9 | 0 |
Obesity is highly correlated with the dysbiosis of intestinal microbiota, and bifidobacteria are one of the soft targets of this metabolic syndrome. The aim of this study is to evaluate the efficacy of Bifidobacterium sp. MKK4 and rice-based fermented foods on physical, haematological, gut microbiota and lypogenic-lypolytic marker genes in diet-induced obese mice. Adult male mice (21±0.7 g) were randomly divided into four groups (n=10) according to the type of diet: normal diet (ND), high fat diet (HFD), HFD supplemented with Bifidobacterium sp. MKK4 and HFD supplemented with MKK4 associated rice-fermented food. 8 weeks of bacterial therapy in the obese mice resulted in significant reduction of body and organ weights, improved serum levels of glucose, triglyceride and cholesterol, the histological structure of the liver (steatosis), and re-establishment of gut Lactobacillus, Bifidobacterium and Bacteroides species. The bacterial therapy led to up-regulation of lipolytic transcription factors, such as peroxisome proliferator-activated receptor (PPAR)-α, PPAR-δ, and their regulated gene products in fatty acid metabolism and glucose uptake, such as acyl-CoA oxidase, carnitine palmitoyl-transferase-1, uncoupling protein-3 and glucose transporter-4. Concomitantly, both adipocytogenesis and fatty acid synthesis were arrested as reflected by the down-regulation of sterol regulatory element binding protein-1c, acetyl-CoA carboxylase, fatty acid synthase and tumour necrosis factor alpha genes. The effectiveness of the fermented product was more profound than the single bacterium. These data provide experimental support with regard to the use of Bifidobacterium sp. MKK4 as a natural therapeutic agent to control obesity.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 185 | 172 | 25 |
Full Text Views | 12 | 6 | 0 |
PDF Views & Downloads | 10 | 9 | 0 |