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A validated approach for collecting fine-wire electromyographic recordings in four canine shoulder muscles during highly dynamic tasks

In: Comparative Exercise Physiology
Authors:
K.L. Cullen Biophysics Interdepartmental Group, University of Guelph, Guelph, ON N1G 2W1, Canada
Institute for Work & Health, 481 University Ave, Toronto, ON M5G 2E9, Canada

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J.P. Dickey Biophysics Interdepartmental Group, University of Guelph, Guelph, ON N1G 2W1, Canada
School of Kinesiology, Faculty of Health Sciences, Western University, London, ON N6A 5B9, Canada

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S.H.M. Brown Biophysics Interdepartmental Group, University of Guelph, Guelph, ON N1G 2W1, Canada
Department of Human Health and Nutritional Sciences, College of Biological Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada

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S.G. Nykamp Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada

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L.R. Bent Department of Human Health and Nutritional Sciences, College of Biological Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada

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J.J. Thomason Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada

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N.M.M. Moens Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada

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This study investigated the feasibility of obtaining ultrasound-guided intramuscular fine-wire electromyographic (fEMG) recordings from four canine shoulder muscles during highly dynamic activities. Four cadaveric canines were utilised to confirm the appropriate anatomical landmarks and the use of real time ultrasound guidance for electrode placement for four shoulder muscles: Biceps Brachii (BB), Supraspinatus (SP), Infraspinatus (IF), and Triceps Brachii – Long Head (TBLH). Electromyographic activity of the left BB, S P, IF, and TBLH was then recorded in two research dogs while walking and trotting to refine the data collection procedures. Finally, the full experimental protocol was piloted with two client-owned, specially-trained agility dogs, confirming the feasibility of collecting fEMG recordings while performing dynamic, highly-specific agility-related tasks and verifying our EMG amplitude normalisation protocol to enable comparisons across muscles and performance tasks. We present specific guidelines regarding the placement of fEMG electrodes and data collection/normalisation procedures to enable investigations of muscle activation during dynamic activities.

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